Allelic relationships at the pvr1 locus in Capsicum
              annuum

Lee, Je Min; Jahn, Molly M.; Yeam, Inhwa

doi:10.1007/s10681-013-0967-2

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…A similar case has been observed in tomato (Mazier et al ., ). Genetic approaches using natural resistance alleles (Lee et al ., ) or transgenesis (Kang et al ., ; Cavatorta et al ., ) suggest that resistance alleles act in a dominant manner over susceptibility alleles, so the mechanism is probably more complex than initially thought.…”

Section: Discussionmentioning

confidence: 98%

“…However, many recent studies have shown that eIF4E-based resistance may behave in a dominant manner. Notably, Ca-eIF4E1-pvr2 resistant alleles act in a dominant manner in F 1 populations of pepper (Lee et al, 2013). Likewise, transgenic over-expression of eIF4E1 resistant alleles in tomato or potato (Solanum tuberosum) generates dominant resistance in a susceptible plant background Cavatorta et al, 2011).…”

Section: Combination Of Knockout Mutations Affecting Eif4e1 and Eif4ementioning

confidence: 99%

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A TILLING approach to generate broad‐spectrum resistance to potyviruses in tomato is hampered by eIF4E gene redundancy

et al. 2016

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SUMMARYGenetic resistance to pathogens is important for sustainable maintenance of crop yields. Recent biotechnologies offer alternative approaches to generate resistant plants by compensating for the lack of natural resistance. Tomato (Solanum lycopersicum) and related species offer a model in which natural and TILLINGinduced potyvirus resistance alleles may be compared. For resistance based on translation initiation factor eIF4E1, we confirm that the natural allele Sh-eIF4E1 PI24 -pot1, isolated from the wild tomato species Solanum habrochaites, is associated with a wide spectrum of resistance to both potato virus Y and tobacco etch virus isolates. In contrast, a null allele of the same gene, isolated through a TILLING strategy in cultivated tomato S. lycopersicum, is associated with a much narrower resistance spectrum. Introgressing the null allele into S. habrochaites did not extend its resistance spectrum, indicating that the genetic background is not responsible for the broad resistance. Instead, the different types of eIF4E1 mutations affect the levels of eIF4E2 differently, suggesting that eIF4E2 is also involved in potyvirus resistance. Indeed, combining two null mutations affecting eIF4E1 and eIF4E2 re-establishes a wide resistance spectrum in cultivated tomato, but to the detriment of plant development. These results highlight redundancy effects within the eIF4E gene family, where regulation of expression alters susceptibility or resistance to potyviruses. For crop improvement, using loss-of-function alleles to generate resistance may be counter-productive if they narrow the resistance spectrum and limit growth. It may be more effective to use alleles encoding functional variants similar to those found in natural diversity.

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Section: Discussionmentioning

confidence: 98%

Section: Combination Of Knockout Mutations Affecting Eif4e1 and Eif4ementioning

confidence: 99%

A TILLING approach to generate broad‐spectrum resistance to potyviruses in tomato is hampered by eIF4E gene redundancy

et al. 2016

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“…The dual effect of the PVY K 472 E mutation in resistance breakdown may be coincidental, with the same NIb domain being involved in physical interactions with two different plant ligands involved in either a recessive or in a dominant form of resistance. Alternatively, a resistance gene or different members of the same gene family may behave as dominant or recessive depending on their allelic forms or the alleles present on the other paralogue genes, as has been shown in the case of the eIF4E family [ 22 , 23 ]. A third hypothesis could be based on a gene-for-gene resistance mechanism as described by the guard model [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of Potato virus Y Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in Capsicum annuum

Moury

Michon

Simon

et al. 2023

Viruses

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The dominant Pvr4 gene in pepper (Capsicum annuum) confers resistance to members of six potyvirus species, all of which belong to the Potato virus Y (PVY) phylogenetic group. The corresponding avirulence factor in the PVY genome is the NIb cistron (i.e., RNA-dependent RNA polymerase). Here, we describe a new source of potyvirus resistance in the Guatemalan accession C. annuum cv. PM949. PM949 is resistant to members of at least three potyvirus species, a subset of those controlled by Pvr4. The F1 progeny between PM949 and the susceptible cultivar Yolo Wonder was susceptible to PVY, indicating that the resistance is recessive. The segregation ratio between resistant and susceptible plants observed in the F2 progeny matched preferably with resistance being determined by two unlinked recessive genes independently conferring resistance to PVY. Inoculations by grafting resulted in the selection of PVY mutants breaking PM949 resistance and, less efficiently, Pvr4–mediated resistance. The codon substitution E472K in the NIb cistron of PVY, which was shown previously to be sufficient to break Pvr4 resistance, was also sufficient to break PM949 resistance, a rare example of cross-pathogenicity effect. In contrast, the other selected NIb mutants showed specific infectivity in PM949 or Pvr4 plants. Comparison of Pvr4 and PM949 resistance, which share the same target in PVY, provides interesting insights into the determinants of resistance durability.

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“…Although both the Ty-1 and Ty-2 loci clearly show dominantly inherited resistance [ 17 , 31 ], it is possible that subtle differences may exist between homozygous and heterozygous resistance. Such a possibility has been suggested for potyviral resistance in peppers [ 44 ]. It is also possible that other host factors, in addition to the known major TYLCV resistance genes in these genotypes, affect viral resistance.…”

Section: Discussionmentioning

confidence: 99%

Development and Application of Gene-Specific Markers for Tomato Yellow Leaf Curl Virus Resistance in Both Field and Artificial Infections

Lee

Chung

Lee

et al. 2020

Plants

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Tomato yellow leaf curl virus (TYLCV) is a disease that is damaging to tomato production worldwide. Resistance to TYLCV has been intensively investigated, and single resistance genes such as Ty-1 have been widely deployed in breeding programs. However, resistance-breaking incidences are frequently reported, and achieving durable resistance against TYLCV in the field is important. In this study, gene-specific markers for Ty-2 and ty-5, and closely-linked markers for Ty-4 were developed and applied to distinguish TYLCV resistance in various tomato genotypes. Quantitative infectivity assays using both natural infection in the field and artificial inoculation utilizing infectious TYLCV clones in a growth chamber were optimized and performed to investigate the individual and cumulative levels of resistance. We confirmed that Ty-2 could also be an effective source of resistance for TYLCV control, together with Ty-1. Improvement of resistance as a result of gene-pyramiding was speculated, and breeding lines including both Ty-1 and Ty-2 showed the strongest resistance in both field and artificial infections.

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Allelic relationships at the pvr1 locus in Capsicum annuum

Cited by 6 publications

References 30 publications

A TILLING approach to generate broad‐spectrum resistance to potyviruses in tomato is hampered by eIF4E gene redundancy

A TILLING approach to generate broad‐spectrum resistance to potyviruses in tomato is hampered by eIF4E gene redundancy

A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of Potato virus Y Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in Capsicum annuum

Development and Application of Gene-Specific Markers for Tomato Yellow Leaf Curl Virus Resistance in Both Field and Artificial Infections

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