Allelic Discrimination of Factor V Leiden Using the GeneAmp® 5700 Sequence Detection System

Monk, Sarah; Duckworth, A. W.; Farrugia, Joanna; Copplestone, J. A.; Rule, Simon

doi:10.1055/s-0037-1613360

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“…They require only a small fraction of a single aphid homogenate allowing tests to be run in conjunction with other assays. Allelic discrimination using quantitative real-time PCR has been successfully used in a number of systems (Monk et al, 2002;McGuigan and Ralston, 2002;Sevall, 2001;Glaab and Skopek, 1999;Abbaszadegan et al, 1997). The methods previously used for determining kdr and super-kdr genotypes in M. persicae, PCR amplification of specific alleles (PASA) (Guillemaud et al, 2003), single-strand conformation polymorphism (SSCP) and sequencing, are not high throughput and require postamplification manipulations which increase the time and labour required.…”

Section: Discussionmentioning

confidence: 99%