High-throughput detection of knockdown resistance in Myzus persicae using allelic discriminating quantitative PCR

Inheritance of L1014F and M918T sodium channel mutations associated with pyrethroid resistance in Myzus persicae

“…Clone 2169G and other M. persicae clones heterozygous for L1014F and M918T have been shown to exhibit higher resistance to pyrethroids than the ones homozygous for F1014 alone (Anstead et al 2004;Eleftherianos et al 2008). All double heterozygote (FT/LM) aphids tested in this study also showed strong pyrethroid resistance, despite knock-down resistance mutations usually being considered to be recessive in their expression (Soderlund & Knipple 2003).…”

“…Resistance conferred by L1014F is termed knock-down resistance or kdr. An additional mutation, a methionine-to-threonine replacement (M918T) in the short cytoplasmic domain between segments IIS4 and IIS5 of the channel protein, has also been detected together with the L1014F mutation in some M. persicae clones (Anstead et al 2004;Eleftherianos et al 2008). This confers an enhanced form of pyrethroid resistance termed super-kdr.…”

Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae)

“…PCR-based screening assays have already been developed to allow field detection of target-site mutations conferring resistance to pyrethroid, cyclodiene and Bacillus thuringiensis insecticides (Anstead et al, 2004;Bass et al, 2004;Brun-Barale et al, 2005;Tabashnik et al, 2006;Gahan et al, 2007;Rinkevich et al, 2007). DNA screening has several advantages compared with bioassays.…”

Spatial and temporal dynamics of Myzus persicae clones in fields and suction traps

“…MACE activity was tested by kinetic enzyme assay in the presence or absence of a diagnostic concentration of insecticides ( Moores et al , 1994 ). Finally, kdr and skdr were determined by highthroughput real-time PCR techniques ( Anstead et al , 2004 ).…”

“…DNA from clone F samples was tested for insecticide resistance status and the samples were all found to be R 2/3 esterase and homozygous for kdr (kdr/kdr) ( Foster et al , 2000;Anstead et al , 2004 ). Clone G, an insecticide sensitive clone, was found once in 1995 and once in 2003.…”