Allele frequency estimation of BLAD (Bovine Leukocyte Adhesion Deficiency) in dairy cattle in Enrekang regency South Sulawesi Indonesia

Factor XI Deficiency (FXID) is a genetic defect due to an autosomal recessive allele in dairy cows, thus cause a large economic and productivity loss in livestock. The purpose of this study was to identify the distribution of recessive alleles of FXID in dairy cows in Enrekang regency using the PCR method. A total of 80 DNA samples were isolated from the FH dairy cow blood sample collected from the Enrekang dairy farms (50 samples from Cendana district and 30 samples from Anggeraja District). All DNA samples were amplified by the PCR technique. The identification of the FXID carrier allele was calculated based on the genotype and allele frequencies. Results showed that 1 of 50 samples from Cendana district was FXID heterozygous while none of 30 samples from Anggeraja district showed FXID alleles. This study concluded that there was a normal heterozygous (Ff) FXID carrier in dairy cow population in Enrekang Regency with the allele frequency was 1.25%.

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Identification of Bovine Citrullinaemia (BC) disease-carrying alleles in Dairy cattle from Enrekang regency

Kamaruddin

Dagong

Sonjaya

2020

IOP Conf. Ser.: Earth Environ. Sci.

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Bovine Citrullinaemia (BC) disease is a genetic disorder that causes increased ammonia in the blood circulation and lethal. In order not to spread the disease, it is advisable to avoid the spread of Bovine Citrullinaemia (BC) disease-carrying alleles in the dairy cow population. This study aims to identify the distribution of allele carriers of Bovine Citrullinaemia (BC) in dairy cows in the Enrekang regency using PCR-RFLP method. A total of 80 DNA samples originating from the dairy development center in Enrekang (50 heads from Cendana and 30 heads from Angeraja district). DNA samples were amplified by PCR, PCR products were then cut using AvaII restriction enzymes. Identification of BC alleles carriers were calculated based on genotype and allele frequencies. These research found that about 0.6% of Bovine Citrullinaemia recessive allele frequencies in Enrekang and still relatively very low. Although the frequency of BC alleles identified is quite low (0.6), the spread of these alleles needs to be anticipated because they can influence the development of dairy cattle populations in the future. It is necessary to identify local cows in South Sulawesi to prevent the spread of BC genetic disorders in the population thereby reducing losses to farmers.

show abstract

Allele frequency estimation of BLAD (Bovine Leukocyte Adhesion Deficiency) in dairy cattle in Enrekang regency South Sulawesi Indonesia

Cited by 3 publications

References 10 publications

Identification of factor XI deficiency, complex vertebral malformation, and Bovine Leukocyte adhesion deficiency carriers in Indonesian Pasundan bull

Identification of factor XI deficiency, complex vertebral malformation, and Bovine Leukocyte adhesion deficiency carriers in Indonesian Pasundan bull

Factor XI Deficiency (FXID) alleles distribution in dairy cow population in Enrekang regency, South Sulawesi

Identification of Bovine Citrullinaemia (BC) disease-carrying alleles in Dairy cattle from Enrekang regency

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