All-Trans Retinoic Acid Promotes Osteogenic Differentiation and Bone Consolidation in a Rat Distraction Osteogenesis Model

Weng, Zhenjun; Wang, Chunyang; Zhang, Cheng; Xu, Jia; Chai, Yimin; Jia, Yuechen; Han, Pei; Wen, Gen

doi:10.1007/s00223-018-0501-6

Cited by 27 publications

(13 citation statements)

References 41 publications

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“…Long bone reconstruction uses a distraction osteogenesis model—a surgical model that creates a gap in a long bone (usually tibia), using continuous traction on the osteotomy ends. This model was used with various rodent species (e.g., mouse [184,185,186,187], rat [188,189,190,191], and rabbit [192,193,194]) and even larger mammalians (e.g., sheep [195,196] or goats [197]). Endo-osseous implants are used for dental reconstruction or oromaxilofacial reconstruction after tumor resection [198], but their biocompatibility also needs testing [199].…”

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

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Recently, a large spectrum of biomaterials emerged, with emphasis on various pure, blended, or doped calcium phosphates (CaPs). Although basic cytocompatibility testing protocols are referred by International Organization for Standardization (ISO) 10993 (parts 1–22), rigorous in vitro testing using cutting-edge technologies should be carried out in order to fully understand the behavior of various biomaterials (whether in bulk or low-dimensional object form) and to better gauge their outcome when implanted. In this review, current molecular techniques are assessed for the in-depth characterization of angiogenic potential, osteogenic capability, and the modulation of oxidative stress and inflammation properties of CaPs and their cation- and/or anion-substituted derivatives. Using such techniques, mechanisms of action of these compounds can be deciphered, highlighting the signaling pathway activation, cross-talk, and modulation by microRNA expression, which in turn can safely pave the road toward a better filtering of the truly functional, application-ready innovative therapeutic bioceramic-based solutions.

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Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

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“…Despite being effective, the DO procedure necessitates a prolonged consolidation phase that can result in high complication rates [ 13 ], restricting its widespread clinical implementation. A range of factors has been explored as potential adjuvant therapies capable of improving DO-related bone regeneration, including growth factors [ 14 ], laser therapy [ 15 ], hormone [ 16 , 17 ], bisphosphonates [ 18 ], and stem cell therapy [ 4 ]. While many of these approaches have been found to accelerate bone regeneration to varying extents, there are concerns regarding their safety or feasibility, which have limited their clinical use [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Panax notoginseng Saponin Promotes Bone Regeneration in Distraction Osteogenesis via the TGF-β1 Signaling Pathway

Liu

Zhao

Jiang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Distraction osteogenesis (DO) is an efficient strategy that is employed for the treatment of large bone defects in craniomaxillofacial surgery. Despite its utility, however, DO is associated with a prolonged consolidation phase and a high complication rate that hinder its more widespread utilization. Panax notoginseng saponin (PNS) is a traditional Chinese medicine that is frequently administered for the treatment of a range of conditions. Herein, we explored the ability of PNS treatment to influence osteogenic differentiation using both rabbit bone marrow mesenchymal cells (BMSCs) and a model of mandibular DO. BMSC proliferation was assessed via CCK-8 assay, while osteogenic differentiation was monitored through ALP and alizarin red S staining. A PCR approach was used to evaluate the expression of genes associated with osteogenesis (ALP, Runx2, and OCN) and genes linked to the TGF pathway (TβR-II, SMAD2, SMAD3, and PPM1A). For in vivo experiments, treated BMSCs were locally injected into the DO gap, with PNS being injected into treated rabbits every other day throughout the experimental period. The quality of the regenerative process was assessed via scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray imaging, and hematoxylin and eosin (H&E) staining. These analyses revealed that PNS was able to promote BMSC osteogenesis and mandibular generation, driving the upregulation of osteogenesis-related genes at the mRNA levels through the modulation of the TGF-β1/Smad pathway. Consistently, the overexpression or silencing of TβR-II in PNS-treated BMSCs was sufficient to modulate their osteogenic potential. Analyses of in vivo mandibular DO outcomes revealed significantly augmented new bone growth in the PNS-treated group relative to control animals, with maximal osteogenesis in the group overexpressing rabbit TβR-II. Together, these results highlight the PNS as a promising and cost-effective therapeutic tool with the potential to enhance bone regeneration in clinical contexts through the modulation of the TGF-β1/Smad pathway.

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“…Vitamin A is a fat-soluble vitamin obtained from the diet either as preformed vitamin A (mainly retinol and retinyl esters) in foods of animal origin or as provitamin A carotenoids in plant-derived foods [190,191]. Inside target cells, retinol is oxidized to all-trans-retinoic acid (ATRA) (the bioactive metabolite of vitamin A), which binds cellular retinoic acid-binding proteins (CRABP) and specific nuclear receptors (i.e., retinoic acid receptor/retinoid X receptor (RARs/RXRs)) that, once ligand activated, induce transcription of specific genes [192] crucial for the modulation of differentiation, proliferation, inflammation and apoptosis processes [192][193][194][195]. Several studies have demonstrated the key role of ATRA in the regulation of bone cell function and physiological bone remodeling.…”

Section: Vitamin a Effects Of Retinoids On Osteoblast Culturesmentioning

confidence: 99%

Effects of Extracellular Osteoanabolic Agents on the Endogenous Response of Osteoblastic Cells

Alloisio

Ciaccio

Fasciglione

et al. 2021

Cells

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The complex multidimensional skeletal organization can adapt its structure in accordance with external contexts, demonstrating excellent self-renewal capacity. Thus, optimal extracellular environmental properties are critical for bone regeneration and inextricably linked to the mechanical and biological states of bone. It is interesting to note that the microstructure of bone depends not only on genetic determinants (which control the bone remodeling loop through autocrine and paracrine signals) but also, more importantly, on the continuous response of cells to external mechanical cues. In particular, bone cells sense mechanical signals such as shear, tensile, loading and vibration, and once activated, they react by regulating bone anabolism. Although several specific surrounding conditions needed for osteoblast cells to specifically augment bone formation have been empirically discovered, most of the underlying biomechanical cellular processes underneath remain largely unknown. Nevertheless, exogenous stimuli of endogenous osteogenesis can be applied to promote the mineral apposition rate, bone formation, bone mass and bone strength, as well as expediting fracture repair and bone regeneration. The following review summarizes the latest studies related to the proliferation and differentiation of osteoblastic cells, enhanced by mechanical forces or supplemental signaling factors (such as trace metals, nutraceuticals, vitamins and exosomes), providing a thorough overview of the exogenous osteogenic agents which can be exploited to modulate and influence the mechanically induced anabolism of bone. Furthermore, this review aims to discuss the emerging role of extracellular stimuli in skeletal metabolism as well as their potential roles and provide new perspectives for the treatment of bone disorders.

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All-Trans Retinoic Acid Promotes Osteogenic Differentiation and Bone Consolidation in a Rat Distraction Osteogenesis Model

Cited by 27 publications

References 41 publications

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

Panax notoginseng Saponin Promotes Bone Regeneration in Distraction Osteogenesis via the TGF-β1 Signaling Pathway

Effects of Extracellular Osteoanabolic Agents on the Endogenous Response of Osteoblastic Cells

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