All-optical automatic pollen identification: Towards an operational system

Crouzy, Benoît; Stella, Michelle; Konzelmann, Thomas; Calpini, B.; Clot, Bernard

doi:10.1016/j.atmosenv.2016.05.062

Cited by 131 publications

(136 citation statements)

References 29 publications

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“…This trend was also noted for individual species, such as grass pollen (r = 0.83), amongst others. These observations are of great interest given that grass pollen is generally considered to be the greatest contributor to hay fever [138]. Recently, an extended version of the PA-300 has been developed, the PA-1000.…”

Section: Pa-300mentioning

confidence: 99%

“…Only one peer-reviewed publication is currently available on the use of the PA-300 [138], which sought to analyze the performance of the PA-300 with regard to pollen identification and airborne particle concentrations. The spectral measurements were made in parallel to a manual reference measurement instrument (Hirst-type volumetric sampler/optical microscopy) during a Swiss pollen season (14th February-31st August, 2016).…”

Section: Pa-300mentioning

confidence: 99%

See 1 more Smart Citation

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Fennelly

Sewell²,

Prentice

et al. 2017

Atmosphere

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Primary biological aerosol particles (PBAP) encompass many particle types that are derived from several biological kingdoms. These aerosol particles can be composed of both whole living units such as pollen, bacteria, and fungi, as well as from mechanically formed particles, such as plant debris. They constitute a significant proportion of the overall atmospheric particle load and have been linked with adverse health issues and climatic effects on the environment. Traditional methods for their analysis have focused on the direct capture of PBAP before subsequent laboratory analysis. These analysis types have generally relied on direct optical microscopy or incubation on agar plates, followed by time-consuming microbiological investigation. In an effort to address some of these deficits, real-time fluorescence monitors have come to prominence in the analysis of PBAP. These instruments offer significant advantages over traditional methods, including the measurement of concentrations, as well as the potential to simultaneously identify individual analyte particles in real-time. Due to the automated nature of these measurements, large data sets can be collected and analyzed with relative ease. This review seeks to highlight and discuss the extensive literature pertaining to the most commonly used commercially available real-time fluorescence monitors (WIBS, UV-APS and BioScout). It discusses the instruments operating principles, their limitations and advantages, and the various environments in which they have been deployed. The review provides a detailed examination of the ambient fluorescent aerosol particle concentration profiles that are obtained by these studies, along with the various strategies adopted by researchers to analyze the substantial data sets the instruments generate. Finally, a brief reflection is presented on the role that future instrumentation may provide in revolutionizing this area of atmospheric research.

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Section: Pa-300mentioning

confidence: 99%

Section: Pa-300mentioning

confidence: 99%

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Fennelly

Sewell²,

Prentice

et al. 2017

Atmosphere

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show abstract

“…On the one hand, a proper visualization of the raw aerobiological data may be a great help before analysing the data (plot_normsummary and plot_summary functions). In addition, the user may carry (Wickham, 2016) and interactive plots (Chang, Cheng, Allaire, Xie, & McPherson, 2018;Sievert, 2018) for improving the interpretation of the results.…”

Section: Visualization Of the Results: Static And Interactive Plotsmentioning

confidence: 99%

“…New aerobiological methods with automatic sampling devices have been developed over the last years and new devices are under improvement (Crouzy, Stella, Konzelmann, Calpini, & Clot, ; Kawashima et al, ; Oteros et al, ). Such novel techniques will require new methods for analysing the huge amount of real‐time aerobiological data provided (Ghitarrini et al, ).…”

Section: Introductionmentioning

confidence: 99%

AeRobiology: The computational tool for biological data in the air

2019

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Aerobiological databases are constantly increasing. Many of them contain long and extensive time series of data which are very difficult and tedious to manage. The development of new real‐time automatic sampling devices also requires new tools to reduce time of calculations and data management. In this sense, the AeRobiology r package has been implemented to accelerate and facilitate these tasks. This package was structured in three sections based on (a) the checking of the database, (b) calculation of the main aerobiological indexes and (c) visualization of the results. The AeRobiology package contains numerous functions which, in conjunction, solve the main general tasks that scientists must assume for the analysis of the biological data. The package is freely distributed under GNU General Public License and can directly be installed from CRAN ( http://cran.r-project.org/). The reference manual is available at https://cran.r-project.org/web/packages/AeRobiology. Contact: aerobiology.package@gmail.com.

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“…There are currently a number of systems being developed with the aim of producing real-time (or almost real-time) aerobiological data (e.g. Kawashima et al (2007), Oteros et al (2015), Perring et al (2015), Crouzy et al (2016) and Kawashima et al (2017)) but there is still more scope to work in this area.…”

Section: Knowledge Gaps and Research Needs/prioritiesmentioning

confidence: 99%

Aerobiology in the International Journal of Biometeorology, 1957–2017

2017

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Aerobiology and biometeorology are related fields. Here we provide a broad review of aerobiology articles published in the International Journal of Biometeorology (IJB) over the past 60 years. We consider how the quantity of such work has varied over this period as well as which regions and countries have been the focus of such work, and where there is a relative paucity. We then focus on a number of highlights and themes in this research, including aerobiology and climate change and aerobiological modelling and forecasting. While much of the article focusses on airborne pollen research, we also discuss the extent to which other airborne organic particles such as fungal spores and bacteria have been the focus of research published in IJB. Also considered are knowledge gaps and research needs and priorities with respect to the field of aerobiology. While the IJB has been one of the main platforms for presenting aerobiological research over recent decades, the article highlights the need for the field of aerobiology to embrace new sampling technologies such as spectral analysis and next-generation sequencing to identify and quantify airborne biological particles.

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All-optical automatic pollen identification: Towards an operational system

Cited by 131 publications

References 29 publications

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

AeRobiology: The computational tool for biological data in the air

Aerobiology in the International Journal of Biometeorology, 1957–2017

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