Alkaloids from Coptis chinensis root promote glucose uptake in C2C12 myotubes

Yang, Te-Chun; Chao, Hsin-Fan; Shi, Li-Shian; Chang, Tsu‐Chung; Lin, Hang-Ching; Chang, Wen-Liang

doi:10.1016/j.fitote.2014.01.008

Cited by 41 publications

(41 citation statements)

References 25 publications

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“…Berberine, an isoquinoline alkaloid isolated from several traditional Chinese herbal medicines, exhibits strong antimicrobial activity and has demonstrated therapeutic effects against diarrhea and utility as a heat‐clearing and detox agent in traditional Chinese herbal medicine applications. Modern research on the pharmacological effects of berberine have demonstrated that berberine also possesses activity that impacts the efficacy of antioxidants and exerts hepatoprotective, blood glucose‐lowering, lipid‐lowering, and antineoplastic and antiarrhythmic effects.…”

Section: Introductionmentioning

confidence: 99%

Berberine impairs embryonic development in vitro and in vivo through oxidative stress‐mediated apoptotic processes

Huang

Ho³

et al. 2017

Environmental Toxicology

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Berberine, an isoquinoline alkaloid isolated from several traditional Chinese herbal medicines, has been shown to suppress growth and induce apoptosis in some tumor cell lines. However, berberine has also been reported to attenuate H O -induced oxidative injury and apoptosis. The basis for these ambiguous effects of berberine-triggering or preventing apoptosis-has not been well characterized to date. In the current investigation, we examined whether berberine exerts cytotoxic effects on mouse embryos at the blastocyst stage and affects subsequent embryonic development in vitro and in vivo. Treatment of blastocysts with berberine (2.5-10 μM) induced a significant increase in apoptosis and a corresponding decrease in trophectoderm cell number. Moreover, the implantation success rate of blastocysts pretreated with berberine was lower than that of their control counterparts. Pretreatment with berberine was also associated with increased resorption of postimplantation embryos and decreased fetal weight. In an animal model, intravenous injection of berberine (2, 4, or 6 mg/kg body weight/d) for 4 days resulted in apoptosis of blastocyst cells and early embryonic developmental injury. Berberine-induced injury of mouse blastocysts appeared to be attributable to oxidative stress-triggered intrinsic apoptotic signaling processes that impaired preimplantation and postimplantation embryonic development. Taken together, our results clearly demonstrate that berberine induces apoptosis and retards early preimplantation and postimplantation development of mouse embryos, both in vitro and in vivo.

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Section: Introductionmentioning

confidence: 99%

Berberine impairs embryonic development in vitro and in vivo through oxidative stress‐mediated apoptotic processes

Huang

Ho³

et al. 2017

Environmental Toxicology

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“…Type 2 diabetes is characterized by insulin resistance and a progressive decline in b-cell function (Laakso 2001). It has been reported that insulin resistance is an important factor in the pathogenesis of type 2 diabetes (Meier & Bonadonna 2013), which is characterized not only by decreased responsiveness of the peripheral target tissues (such as liver, adipose tissue, and skeletal muscle) to insulin but also by remarkable decrease in glucose uptake and utilization (Yang et al 2014). Thus, increasing glucose uptake of the above three key tissues is one of the effective therapeutic approaches for treating diabetes.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic activity of Helicteres angustifolia root

Cheng

Zhang

2016

Pharmaceutical Biology

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ContextThe root of Helicteres angustifolia L. (Sterculiaceae) has been used as folk herbal drug to treat cancer, bacterial infections, inflammatory, and flu in China. However, there is no report on its antidiabetic activity. Objective This study evaluates the antidiabetic activity of ethanol extract from H. angustifolia root. Materials and methods The promoting effect of H. angustifolia root ethanol extract (25, 50, and 100 mg/mL) on glucose uptake was evaluated using HepG2 cell, differentiated C2C12 myotubes, and differentiated 3T3-L1 adipocytes. The antidiabetic activity of the extract was assessed in vivo using STZ-induced diabetic rats by orally administration of the extract (200 and 400 mg/kg b.w.) once per day for 28 d. Blood glucose, TG, TC, TP, HDL-C, UA, BUN, AST, ALT, insulin, and HOMA-IR were analyzed. Results The results showed that the extract increased glucose uptake in C2C12 myotubes and 3T3-L1 adipocytes with an IC 50 value of 79.95 and 135.96 mg/mL, respectively. And about 12%, 19%, and 10% (p 5 0.05) in HepG2 cells when compared with the control at the concentration of 25, 50, and 100 mg/mL, respectively. After 28 days' treatment with the extract, significant reduction was observed in blood glucose, HOMA-IR, TC, TG, UA, BUN, AST, and ALT levels, while the levels of TP and HDL cholesterol increased. Discussion and conclusion These results suggest that H. angustifolia root ethanol extract possess potent antidiabetic activity, which is the first report on antidiabetic activity of this plant.

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“…Those two proteins able to activate each other, AMPK activate SIRT‐1 by elevation the concentration of nicotinamide phosphoribosyltransferase and SIRT‐1 stimulates AMPK through deacetylation of serine‐threonine kinase LKB1 (Hardie, ; Sun et al, ). Also, BER has the capacity to recover insulin resistance syndrome through other mechanisms, where it protects β‐cell of islets of Langerhans from damage, allows glucose uptake by skeletal muscle, improves hepatic gluconeogenesis and decreases the concentration of lipids in the blood (Pirillo & Catapano, ; Yang et al, ). As a result of SIRT‐1 and AMPK pathway activation, adiponectin concentration was restored after BER‐chloride therapy in our study which similar to previous results (Wu et al, ).…”

Section: Discussionmentioning

confidence: 99%

Berberine chloride ameliorated PI3K/Akt‐p/SIRT‐1/PTEN signaling pathway in insulin resistance syndrome induced in rats

et al. 2019

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The liver is the main organ involved in lipid metabolism process and it helps in drug detoxification. Insulin resistance is considered one of risk reasons which lead to several metabolic diseases. Currently, berberine (BER) occupies a huge challenge against multiple diseases with no toxic effect. The present work was aimed to identify, does BER-chloride has a poisonous influence on the liver? and investigating the outcome of BER-chloride on PI3K/Akt-p/SIRT-1/PTEN pathway during insulin resistance syndrome. The insulin resistance model was achieved in experimental female rats via high-fat diet (HFD). Glucose, insulin, lipid profiles, and hepatic oxidative stress parameters were assessed. PI3K, AKt-p, SIRT-1, and PTEN levels in hepatic tissue were determined at genome and protein levels. Further adiponectin concentration was performed in serum, hepatic, and white adipose tissues. Molecular study of fold alteration in insulin, insulin receptor, and retinol binding protein-4 (RBP4) in liver was done. Practical applicationsObesity syndrome causes multiple obstacles in modern years. The current results revealed elevation the body weight of rats, plasma glucose, homeostatic model assessment, glycated hemoglobin, insulin, and lipid profiles concentrations in a group of rats, which nourished HFD for 8 weeks and this rise, was diminished after 2 weeks from BER-chloride administration. Further, BER-chloride improved transaminases enzymes, pro-oxidant, and antioxidant defense system, PI3K, AKt-p, SIRT-1, and PTEN in the liver, with downregulation of hepatic RBP4. Hence, these data provide a crucial message that BER-chloride enhanced both hepatic function and insulin signaling pathways that might be of therapeutic importance to insulin resistance with no harmful effect on the liver. BER-chloride is predicted to be a drug of choice for obesity complications cure. K E Y W O R D Sberberine chloride, hepatotoxicity, insulin receptor, liver

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Alkaloids from Coptis chinensis root promote glucose uptake in C2C12 myotubes

Cited by 41 publications

References 25 publications

Berberine impairs embryonic development in vitro and in vivo through oxidative stress‐mediated apoptotic processes

Berberine impairs embryonic development in vitro and in vivo through oxidative stress‐mediated apoptotic processes

Antidiabetic activity of Helicteres angustifolia root

Berberine chloride ameliorated PI3K/Akt‐p/SIRT‐1/PTEN signaling pathway in insulin resistance syndrome induced in rats

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