Alkaloid patterns in Leucojum aestivum shoot culture cultivated at temporary immersion conditions

Ivanov, Ivan; Georgiev, Vasil; Berkov, Strahil; Pavlov, Atanas

doi:10.1016/j.jplph.2011.09.010

Cited by 34 publications

(29 citation statements)

References 26 publications

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“…Somatic embryogenesis can greatly improve the multiplication rate of in vitro bulbous plants. In the future, plants could be regenerated from somatic embryos and propagated in bioreactors, as previously shown for shoot cultures [14,15]. Therefore, the capacity of Leucojum aestivum embryogenic callus and the impact of both phenolic compounds and Amaryllidaceae alkaloids on the biosynthetic pathway were estimated for the first time under the influence of various auxins (2,4-D, picloram, and dicamba).…”

Section: Introductionmentioning

confidence: 96%

“…The low Amaryllidaceae alkaloid accumulation commonly observed in in vitro cultures of Leucojum aestivum [6,[9][10][11][12][13] was probably due to the fact that the level of the alkaloid biosynthesis is strongly influenced by cellular differentiation [10,[14][15][16]. In particular, enhanced synthesis of galanthamine accompanied development of somatic embryos [17].…”

Section: Introductionmentioning

confidence: 99%

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Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

et al. 2013

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In vitro cultures of Leucojum aestivum are considered as an alternative for the production of galanthamine, which is used for the symptomatic treatment of Alzheimer’s disease. We studied the effects of auxins 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,6-dichloro-o-anisic acid (dicamba) at concentrations of 25 and 50 µM on the induction of embryogenic callus and its capacity to induce somatic embryogenesis and alkaloid accumulation. The embryogenic response of the explants was from 30% for 25 µM of dicamba to 100% for picloram (for both 25 and 50 µM). 2,4-D (50 µM) stimulated greater callus proliferation and somatic embryo induction as compared to the other auxins. Polyethylene glycol (PEG) stimulated somatic embryo maturation. Callus grown on media containing 50 µM of auxins produced fewer phenolic compounds as compared with callus grown on media containing 25 µM of auxins. GC-MS analyses showed seven alkaloids in the in vivo bulbs and two to four in callus culture. Galanthamine was detected in callus cultivated with 2,4-D (25, 50 µM), picloram (25 µM), and dicamba (50 µM). Other alkaloids, trisphaeridine, tazettine, and 11-hydroxyvittatine were accumulated only in callus growing on medium with picloram (50 µM).

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

et al. 2013

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“…It is well known that in vitro culture in temporary immersion bioreactors per se with the optimum time and frequency of immersion is a good method to increase the production of matter and secondary metabolites of plants with high commercial value (Ivanov et al 2012;Georgiev et al 2014). In this sense, it was logical that we observed an increase of secondary metabolites (PhGs and Aucubin) associated to the increase of matter and culture time in plants grown in the control medium (complete N supply).…”

Section: Discussionmentioning

confidence: 89%

MORPHOGENESIS AND SECONDARY METABOLITES PRODUCTION IN THE MEDICINAL PLANT Castilleja tenuiflora Benth. UNDER NITROGEN DEFICIENCY AND STARVATION STRESS IN A TEMPORARY IMMERSION SYSTEM

Cortes¹

2017

Rev.Mex.Ing.Quim.

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G. Inei-Shizukawa, H. A. Velasco-Bedrán, G. F. Gutiérrez-López and H. Hernández-Sánchez Abstract Castilleja tenuiflora Benth, is a medicinal plant that synthesizes secondary metabolites such as phenylethanoid glycosides (PhGs), flavonoids, lignans, and iridoid glycosides with pharmacological properties. Nutrient deficiency stress in in vitro culturing of plants has been shown to be a good biotechnological strategy to stimulate secondary metabolism. Our aim was to evaluate morphogenesis (growth and development) and the production of secondary metabolites in C. tenuiflora shoots in response to stress by nitrogen (N) deficiency and starvation in temporary immersion bioreactors. Shoots were grown in B5 liquid medium with 25.74 mM (control), 1.32, 0.66 (deficiency) and 0 mM (starvation) N concentration. In all treatments, matter and shoot multiplication rate (SMR) decreased over 40%, chlorophylls content decreased, roots length increased, and leaves showed chlorosis respect to control. Under 0.66 mM N deficiency, the enzyme phenylalanine ammonia lyase (PAL) was more active (twice more than control) and total phenolic compounds were enhanced. At day 21: maximum concentration of PhGs (verbascoside and isoverbascoside) and iridoids (aucubin) was observed in 0.66, 1.32 and 0 mM N treatments (283 ± 0.5, 90 ± 0.3 and 12.5 ± 0.1 mg g −1 DM, respectively); these concentrations were 175, 143 and 34% greater than respective controls on the same day. Additionally, presence of anthocyanins was evident in stems of all plants developed under N deficiency and starvation. Despite the fact that, the matter, SMR and chlorophylls decreased because N stress deficiency, the production of secondary metabolites was enhanced, demonstrating that in vitro culture in temporary immersion bioreactors under suitable nutrients deficit such as N is an appropriate strategy to stimulate secondary metabolism in C. tenuiflora. Keywords: abiotic stress, Aucubin, nitrogen starvation, phenylethanoid glycosides, RITA® bioreactor. ResumenCastilleja tenuiflora Benth., es una planta medicinal que sintetiza metabolitos secundarios como feniletanoides glicosilados (PhGs), flavonoides, lignanos, e iridoides; que presentan actividad farmacológica. El estrés por deficiencia de nutrientes en el cultivo de plantas in vitro ha mostrado ser una estrategia biotecnológica apropiada para estimular su metabolismo secundario. En este trabajo se evaluaron la morfogénesis (crecimiento y desarrollo) y la producción de metabolitos secundarios en brotes de C. tenuiflora en biorreactores de inmersión temporal expuestos a estrés por deficiencia y ausencia de nitrógeno (N). El estrés se aplicó a un cultivo de los brotes cultivados en medio líquido B5 con 25.74 mM (control), 1.32, 0.66 (deficiencia) y 0 mM (ausencia) de N. En comparación con el control, todos los tratamientos mostraron una disminución de más del 40% en la biomasa y elíndice de multiplicación de brotes, el contenido de clorofila disminuyó, la longitud de las raíces aumentó y las hojas mostraron clorosis. En defi...

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“…Particularly, there is no need to harvest plants from their natural habitats for the production of biologically active compounds, because tissue culture procedures could supply raw material for such purposes. Some important examples are Galanthus elwesii, Leucojum aestivum, and Narcissus pseudonarcissus [7,8].…”

Section: Digital Signaturementioning

confidence: 99%

Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species

et al. 2017

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The aim of this study was (i) to produce tissue cultures capable of efficient plant regeneration from European naturally occurring protected and/or pharmacologically important Amaryllidaceae species and (ii) to test them for antioxidant activities in order to select tissue cultures that scavenge efficiently oxygen radicals. Bulb explants were collected from Galanthus woronowii, two Leucojum species, and Sternbergia lutea. Leucojum species were Hungarian isolates. Mostly α-naphthalene acetic acid (NAA) and benzyladenine (BA) were used as growth regulator combinations for the induction and maintenance of tissue cultures and further antioxidant activity studies. Galanthus woronowii and L. vernum cultures produced shoots or whole plants via micropropagation (callus stage was observed only sporadically and callus tissue did not contribute to regeneration), whereas L. aestivum and S. lutea produced efficiently whole plants or multiple shoots via embryogenic calli. Total phenolic content, % inhibition of ABTS radical (ABTS*) cation, and peroxidase activities on native polyacrylamide gels were studied and showed differences between cultures. No relationship could be detected between polyphenol content / radical scavenging capacities and H 2 O 2 reducing enzyme activities. For G. woronowii, S. lutea, and a culture line of L. vernum, polyphenol content and ABTS* cation scavenging activities were high and for G. woronowii, comparable to organs of the native plants used as explant sources. Bulbs of native plants showed low radical scavenging activities in general. For L. vernum and L. aestivum tissue cultures grown in the presence of NAA as the sole growth regulator, ABTS* cation scavenging showed low values. Enzymatic antioxidant (pyrogallol peroxidase) activities were low for all cultures and organs of native plants. This study shows the species conservation value of these cultures and highlights the high antioxidant capacity of G. woronowii and S. lutea, attributed to the presence of non-enzymatic scavengers.

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Alkaloid patterns in Leucojum aestivum shoot culture cultivated at temporary immersion conditions

Cited by 34 publications

References 26 publications

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

MORPHOGENESIS AND SECONDARY METABOLITES PRODUCTION IN THE MEDICINAL PLANT Castilleja tenuiflora Benth. UNDER NITROGEN DEFICIENCY AND STARVATION STRESS IN A TEMPORARY IMMERSION SYSTEM

Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species

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