Alkaline proteases: A review

Anwar, Adil; Saleemuddin, M.

doi:10.1016/s0960-8524(97)00182-x

Cited by 230 publications

(137 citation statements)

References 61 publications

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“…The major applications of protease enzymes in detergent formulation are based on the activity and stability at high pH, temperature and the enzyme compatability along with detergents. Detergents available in the international market such as Dynamo ® , Eraplus ® (Procter & Gamble), Tide ® (Colgate Palmolive), contain proteolytic enzymes, the majority of which are produced by members of the genus Bacillus [8].…”

Section: Introductionmentioning

confidence: 99%

Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant

Basavaraju

Kathera

Jasti

2017

J Microbiol Biotech Res

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Section: Introductionmentioning

confidence: 99%

Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant

Basavaraju

Kathera

Jasti

2017

J Microbiol Biotech Res

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“…Detergent proteases account for at least a quarter of all protease sales throughout the world (Anwar and Saleemuddin 1998;Gupta et al 2002;Kumar and Takagi 1999). They are primarily used as detergent additives since they are biodegradable and increase performance/cost ratios (Gupta et al 2002;Kumar and Takagi 1999).…”

Section: Introductionmentioning

confidence: 99%

Zebra blenny (Salaria basilisca) viscera as a source of solvent-stable proteases: characteristics, potential application in the deproteinization of shrimp wastes and evaluation in liquid laundry commercial detergents

et al. 2012

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The present study describes the characterization of crude protease extract from zebra blenny (Salaria basilisca) and its evaluation in liquid detergent and shrimp waste deproteinization. At least five caseinolytic proteases clear bands were observed in zymogram. The crude alkaline protease showed optimum activity at pH 8.0 and 60°C, and it was highly stable over a wide range of pH from 6.0 to 11.0. Proteolytic enzymes showed extreme stability towards non-ionic surfactants (5 % Tween 80 and 5 % Triton X-100) and oxidizing agents (1 % sodium perborate), and relative stability towards anionic surfactant (1 % Sodium dodecyl sulfate (SDS)). They also showed high stability and compatibility with various laundry liquid detergents from Tunisian market. Furthermore, the crude enzyme was stable towards several organic solvents and retained more than 50 % of its original activity after 30 days of incubation at 30°C in the presence of 50 % (v/v) dimethylsulfoxide (DMSO). Further, proteases from zebra blenny viscera were found to be effective in the deproteinization of shrimp wastes. The protein removal after 3 h at 40°C with an enzyme/substrate ratio (E/S) of 5 U/mg protein was about 77 %.

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“…Our results suggest that the E. anchoita viscera and heads could be a potential source of alkaline proteases for certain industrial applications as one of the most important variables in the selection of proteases for detergent industry is their optimum pH (Banerje et al, 1999). The alkaline proteases are known to improve the effectiveness of laundry detergents due to their ability to aid in the removal of protein stains (Anwar and Saleemudin, 1998;Gupta et al, 2002).…”

Section: Temperature and Ph Optimummentioning

confidence: 77%

Alkaline trypsin from the viscera and heads of Engraulis anchoita : partial purification and characterization

Lamas¹,

Yeannes²,

Massa³

2017

bta

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Marine by-products contain valuable protein fractions. To ensure a profitable utilization of wastes from the fish industry, the knowledge about their quality and composition is necessary. Fish digestive proteolytic enzymes from cold-adapted ectothermic organisms have found applications in several industries because their temperature requirements and other characteristics differ from those of homologous proteases from warm-blooded animals. Herein, we describe detection, partial purification, and characterization of proteolytic enzymes from the viscera and heads of Engraulis anchoita. Enzymatic activities of the by-products were assayed using azocaseín as a substrate. To characterize the alkali protein fraction, the activity against inhibitors and their molecular weights were studied. The crude protein extract exhibited maximal activity at pH 8.0 and 60EC. Results relative to the substrate-specific Nabenzoyl-DL-arginine-p-nitroanilide and the 25 kDa molecular weight indicated that the recovered protease was trypsin. The activity showed an increment in presence of SDS and a slight decrease when it was incubated with EDTA. Increasing the concentration of NaCl up to 5% did not significantly decrease the protein's activity. The results obtained suggest that by-products of anchovy industry could be used in the detergents industry.

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Alkaline proteases: A review

Cited by 230 publications

References 61 publications

Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant

Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant

Zebra blenny (Salaria basilisca) viscera as a source of solvent-stable proteases: characteristics, potential application in the deproteinization of shrimp wastes and evaluation in liquid laundry commercial detergents

Alkaline trypsin from the viscera and heads of Engraulis anchoita : partial purification and characterization

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