Alkaline phosphatase activity of a phosphate solubilizing Alcaligenes faecalis, isolated from Mangrove soil

Behera, Bikash Chandra; Yadav, H.; Singh, Santosh Kumar; Sethi, Bijay Kumar; Mishra, Rashmi; Kumari, Sony; Thatoi, Hrudayanath

doi:10.1016/j.biori.2017.01.003

Cited by 60 publications

(35 citation statements)

References 35 publications

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“…Ali-ul-Qader et al (2009) observed an increase in phosphatase concentration at high pNPP concentrations up to 20 mM. This is in agreement with Behera et al (2017) who reported that different pNPP concentrations (0.5À2.5 mg/ml) resulted in an increase in alkaline phosphatase activity with an increase in substrate concentration up to 2 mg/ml. In the present study, we observed an optimum phosphatase production at incubation time of 96 h for all the microorganisms.…”

Section: Discussionsupporting

confidence: 71%

“…In the present study, we observed an optimum phosphatase production at incubation time of 96 h for all the microorganisms. According to Behera et al (2017), the time generally for optimum phosphatase production by bacteria or fungi was after 48 h. Mahesh et al (2015) also reported the optimum phosphatase activity to be 0.688 U/ml after 24 h and noted that phosphatase yield increased with time. These studies are in agreement with our study as all of the tested microorganisms showed increased phosphatase activity with increased incubation time and substrate concentration.…”

Section: Discussionmentioning

confidence: 99%

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Production of phosphatase by microorganisms isolated from discolored painted walls in a typical tropical environment: a Non-Parametric analysis

Obidi

Awe²,

Igwo-Ezikpe

et al. 2018

Arab Journal of Basic and Applied Sciences

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The enzymatic activities occurring on painted walls in a tropical environment have become critical given the rate of aesthetic biodeterioration of such walls. Pigmented heterotrophic microorganisms isolated from discolored painted walls in Lagos, Nigeria were screened for their phosphatase activities. The strains were characterized by morphological methods and 16S rDNA analysis as belonging to the genera Aspergillus, Meyerozyma, Candida, Fusarium, Cerrena and Pseudomonas. The sequences were submitted to NCBI GenBank. Crude extracellular phosphatase from cell-free culture supernatant were reacted in p-nitrophenolphosphate. Subsequently, their alkaline phosphatase activities were compared. All tested strains produced phosphatase at different pH, temperature, incubation time and substrate concentration which were used as biomarkers for microbial metabolic activity. The order of optimal phosphatase production was observed as: Cerrena sp > Aspergillus sp >M. guilliermondii > A. aculeatus > F. proliferatum > P. aeruginosa > C. tropicalis > M. caribbica. Morphological studies and microbial enzymatic activity confirm the hypothesis that heavy pigmentation and phosphatase production are major indices of discoloration on biodeteriorating painted walls in a typical tropical environment like Nigeria. Kruskal-Wallis non-parametric test revealed that there was no statistically significant difference in phosphatase production at the different environmental conditions examined except at different time intervals.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Production of phosphatase by microorganisms isolated from discolored painted walls in a typical tropical environment: a Non-Parametric analysis

Obidi

Awe²,

Igwo-Ezikpe

et al. 2018

Arab Journal of Basic and Applied Sciences

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“…Since the bacterium Pseudomonas is among the main microorganisms catalyzing the solubilization of soil phosphates, the production of this enzyme by this bacterium occurs at an ideal pH between 7 and 8.5. This corresponds to the ideal pH range for the growth of most bacteria and for alkaline phosphatase secretion (BEHERA et al, 2017). The main sources of alkaline phosphatase activity are bacteria, whereas the activity of acid phosphatase is in bacteria, fungi and plants (NARENDRULA-KOTHA; NKONGOLO, 2017).…”

Section: Resultsmentioning

confidence: 99%

Microbial Biomass and Enzymatic Activities in Sandy Soil Cultivated With Lettuce Inoculated With Plant Growth Promoters

et al. 2018

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Plant growth promoter microorganisms have been studied as important tools for increasing crop production. Lettuce is the most consumed hardwood crop in the world. Numerous microorganisms are capable of acting in a beneficial way in the growth of this culture. The objective of the present study was to evaluate the efficacy of Trichoderma and Pseudomonas on the microbial biomass, enzymatic activities in sandy soil and lettuce production. The experimental design was completely randomized with ten replicates and treatments: CONT (absolute control); CM (control with cattle manure fertilization); CMB (with fertilization and Pseudomonas sp.); CMF (with fertilization and T. aureoviride) and CMBF (with fertilization and the two microorganisms combined). The fertilizer used was organic with cattle manure in a dose recommended for the culture. This study evaluated the production of lettuce, microbial biomass and the enzymatic activity of acid phosphatase, alkaline phosphatase and urease. The combined application of CMBF was efficient in increasing lettuce production, because it increased 85% of the cv. Veronica cultivated on sandy soil. The combined use of plant growth promoting microorganisms resulted to an increase in microbial biomass. In lettuce crops, it is recommended to use T. aureoviride URM 5158 and Pseudomonas sp. UAGF 14 in lettuce crops, because improved lettuce production, improves the biochemical quality of soils measured by absolute and specific enzymatic activities per unit of microbial biomass.

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“…Earlier, alkaline phosphatases from two bacterial strains (Escherichia coli and Alcaligenes faecalis) was reported to show activity at 80 and 45 C respectively [37,38]. Similarly, Shah et al [39] reported maximum activity of alkaline phosphatase at 37 C followed by decrease at higher temperatures.…”

Section: Discussionmentioning

confidence: 94%

Isolation and characterization of organophosphorus phosphatases from Bacillus thuringiensis MB497 capable of degrading Chlorpyrifos, Triazophos and Dimethoate

Ambreen

Aziz

2020

Heliyon

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In the current investigation, bacterial strain Bacillus thuringiensis MB497 was examined for production of intracellular and extracellular organophosphorus phosphatase (OPP) enzymes. This strain produced significant amount of extracellular acidic and alkaline phosphatases. Production of neutral phosphatase was negligible. Production of OPP was generally highest at pH 11 and at 45-50 C. However, activity and stability of OPP was highest at 37 C and reduced at higher temperatures. OPP production was decreased after 48 h of incubation. Largely, OPP activity was inhibited by SDS and EDTA and significantly enhanced by metals (Zn þþ , Cu þþ and Cd þþ ). Both acidic and alkaline OPPs were capable of bio-precipitation of selected metals (Ni, Mn, Cr and Cd) up to 86-100%. When used against 50 mg/l of three OP pesticides (Chlorpyrifos, Triazophos, and Dimethoate), 81-94.6% degradation of pesticides was observed by alkaline OPP, while acidic OPP showed less degradation (61-70.5%) within 30 min of incubation.

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Alkaline phosphatase activity of a phosphate solubilizing Alcaligenes faecalis, isolated from Mangrove soil

Cited by 60 publications

References 35 publications

Production of phosphatase by microorganisms isolated from discolored painted walls in a typical tropical environment: a Non-Parametric analysis

Production of phosphatase by microorganisms isolated from discolored painted walls in a typical tropical environment: a Non-Parametric analysis

Microbial Biomass and Enzymatic Activities in Sandy Soil Cultivated With Lettuce Inoculated With Plant Growth Promoters

Isolation and characterization of organophosphorus phosphatases from Bacillus thuringiensis MB497 capable of degrading Chlorpyrifos, Triazophos and Dimethoate

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