Alkaline phosphatase activity in gingival crevicular fluid during human orthodontic tooth movement

Perinetti, Giuseppe; Paolantonio, Michele; D’Attilio, Michele; D'Archivio, Domenico; Tripodi, Domenico; Femminella, Beatrice; Festa, Felice; Spoto, Giuseppe

doi:10.1067/mod.2002.126154

Cited by 93 publications

(125 citation statements)

References 46 publications

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“…This may be due to differing experimental conditions. During orthodontic tooth movement, ALP activity is greater in the tension site than the compression site (21). Considering that new bone formation is observed on the tension side during orthodontic tooth movement, experimental conditions that produce increased ALP activity are more likely similar to the clinical observation.…”

Section: Genes Related To New Bone Formationmentioning

confidence: 96%

Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Ku¹,

Chang²,

Chae³

et al. 2009

BMB Reports

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Orthodontic tooth movement results from the combinational process of both bone resorption and formation in the compressive and tension sides, respectively. However, the genes responsible for new bone formation in tension sides have not been determined. In this study, we used DNA microarray and real-time RT-PCR to identify genes in human periodontal ligament (PDL) cells that undergo significant changes in expression in response to static tensional forces (2 or 12 hours). The genes found were alkaline phospatase (ALP), matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and several collagen genes. Furthermore, an ELISA evaluating the expression of VEGF, type IV collagen and MMP-2 found levels significantly increased after 24 and 72 hours (P ＜ 0.05). ALP activity was also increased after 24 hours (P ＜ 0.05). Collectively, we found the genes up-regulated in our study by the static tensional force are related to osteogenic processes such as matrix synthesis and angiogenesis. [BMB reports 2009; 42(7): 427-432]

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Section: Genes Related To New Bone Formationmentioning

confidence: 96%

Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Ku¹,

Chang²,

Chae³

et al. 2009

BMB Reports

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“…12 Alkaline phosphatase can be obtained from clear fluid excreted from gingival crevicular fluid (GCF). This gingival crevicular fluid can describe body's biological response to periodontal healing process in patients with chronic periodontitis 13 or can stimulate orthodontic mechanism 6,13,14 and can be obtained in various ways, such as micro pipetted or paper strips. 15 Gingival crevicular fluid, moreover, is exudate liquid composed of substances derived from various sources, including microbial dental plaque, inflammatory host cells, and host tissue and serum.…”

Section: Introductionmentioning

confidence: 99%

“…5 Bone remodeling that occurs during orthodontic tooth movement is a biological process that involves an acute inflammatory response in periodontal tissue. 6 In the initial phase of orthodontic tooth movement, an acute inflammatory response is involved together with periodontal vasodilatation and leukocyte migration out of the blood vessels of periodontal ligament. 7 Thus, bone remodeling can be considered as complex process, including bone resorption and bone formation requiring the coordination of osteoclasts, osteocytes and osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

Alkaline phosphatase expression during relapse after orthodontic tooth movement

Pudyani

Asmara

Ana

et al. 2014

Dent. J. (Majalah Kedokteran Gigi)

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“…The reactions were then stopped by adding 5 µl 3M NaOH, and the rates of increase in absorbance were read with a spectrophotometer at 405 nm. 10,15 The relevant control for each analysis consisted of the reagent and the Tris buffer without the sample. Using 18.45 as the p-nitrophenol mM absorptivity, the absorbance was converted into enzyme activity units (1 unit = 1 mmol of p-nitrophenol released per minute at 37 °C) and expressed as total activity in mU/sample.…”

Section: Enzymatic Activity Determinationmentioning

confidence: 99%

Gingival crevicular fluid alkaline phosphatase activity in relation to pubertal growth spurt and dental maturation: A multiple regression study

Perinetti¹,

Contardo²

2016

SEJODR

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Introduction:The identification of the onset of the pubertal growth spurt has major clinical implications when dealing with orthodontic treatment in growing subjects.Aim: Through multivariate methods, this study evaluated possible relationships between the gingival crevicular fluid (GCF) alkaline phosphatase (ALP) activity and pubertal growth spurt and dentition phase. Materials and methods:One hundred healthy growing subjects (62 females, 38 males; mean age, 11.5±2.4 years) were enrolled into this doubleblind, prospective, cross-sectional-design study. Phases of skeletal maturation (pre -pubertal, pubertal, post -pubertal) was assessed using the cervical vertebral maturation method. Samples of GCF for the ALP activity determination were collected at the mesial and distal sites of the mandibular central incisors. The phases of the dentition were recorded as intermediate mixed, late mixed, or permanent. A multinomial multiple logistic regression model was used to assess relationships of the enzymatic activity to growth phases and dentition phases.Results: The GCF ALP activity was greater in the pubertal growth phase as compared to the pre -pubertal and post -pubertal growth phases. Significant adjusted odds ratios for the GCF ALP activity for the pre -pubertal and post -pubertal subjects, in relation to the pubertal group, were 0.76 and 0.84, respectively. No significant correlations were seen for the dentition phase. Conclusions:The GCF ALP activity is a valid candidate as a non -invasive biomarker for the identification of the pubertal growth spurt irrespective of the dentition phase.

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Alkaline phosphatase activity in gingival crevicular fluid during human orthodontic tooth movement

Cited by 93 publications

References 46 publications

Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Alkaline phosphatase expression during relapse after orthodontic tooth movement

Gingival crevicular fluid alkaline phosphatase activity in relation to pubertal growth spurt and dental maturation: A multiple regression study

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