Alkaline phosphatase activities and regulation in three harmful Prorocentrum species from the coastal waters of the East China Sea

Ou, Linjian; Qin, Xianling; Shi, Xiaoyong; Feng, Qingliang; Zhang, Shuwen; Lü, Songhui; Qi, Yuzao

doi:10.1007/s00248-019-01399-3

Cited by 29 publications

(13 citation statements)

References 60 publications

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“…The stronger photosynthetic capacity is probably due to the enhanced activity of alkaline phosphatase of P. donghaiense under P deficiency. Ou et al reported that P. donghaiense suffered P stress and expressed abundant alkaline phosphatase, which might help it efficiently utilize organic phosphorus substrates and outcompete other concurrent species to outburst in the P-deficient East China Sea ( 51 ). We also found that alkaline phosphatase activities markedly increased after algal cells were cultured in P deficiency for 4 days and peaked at day 8, which was 2 times that of the control.…”

Section: Resultsmentioning

confidence: 99%

Nutrient Deficiency and an Algicidal Bacterium Improved the Lipid Profiles of a Novel Promising Oleaginous Dinoflagellate, Prorocentrum donghaiense, for Biodiesel Production

Gui

Chen

Luo

et al. 2021

Appl Environ Microbiol

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The lipid production potential of 8 microalgae species was investigated. Among these eight species, the best strain was a dominant bloom-causing dinoflagellate, Prorocentrum donghaiense ; this species had a lipid content of 49.32±1.99% and exhibited a lipid productivity of 95.47±0.99 mg L −1 d −1 , which was 2-fold higher than the corresponding values obtained for the oleaginous microalgae Nannochloropsis gaditana and Phaeodactylum tricornutum . P. donghaiense, which is enriched in C16:0 and C22:6, is appropriate for commercial DHA production. Nitrogen or phosphorus stress markedly induced lipid accumulation to levels surpassing 75% of the dry weight, increased the C18:0 and C17:1 contents, and decreased the C18:5 and C22:6 contents, and these effects resulted in decreases in the unsaturated fatty-acid levels and changes in the lipid properties of P. donghaiense such that the species met the biodiesel specification standards. Compared with the results obtained under N-deficient conditions, the enhancement in the activity of alkaline phosphatase of P. donghaiense observed under P-deficient conditions could partly alleviate the adverse effects on the photosynthetic system exerted by P deficiency to induce the production of more carbohydrates for lipogenesis. The supernatant of the algicidal bacterium Paracoccus sp. Y42 culture lysed P. donghaiense without decreasing its lipid content, which resulted in facilitation of the downstream oil extraction process and energy savings through the lysis of algal cells. The Y42 supernatant treatment improved the lipid profiles of algal cells by increasing their C16:0, C18:0 and C18:1 contents and decreasing their C18:5 and C22:6 contents, which is favourable for biodiesel production. IMPORTANCE This study demonstrates the high potential of P. donghaiense , a dominant bloom-causing dinoflagellate, for lipid production. Compared with previously studied oleaginous microalgae, P. donghaiense exhibit greater potential for practical application due to its higher biomass and lipid contents. Nutrient deficiency and the algicidal bacterium Paracoccus sp. Y42 could improve the suitability of the lipid profile of P. donghaiense for biodiesel production. Furthermore, Paracoccus sp. Y42 effectively lyse algal cells, which facilitates the downstream oil extraction process for biodiesel production and results in energy savings through the lysing of algal cells. This study provides a more promising candidate for the production of DHA for human nutritional products and of microalgal biofuel, as well as a more cost-effective method for breaking algal cells. The high lipid productivity of P. donghaiense and algal cell lysis by algicidal bacteria contribute to reductions in the production cost of microalgal oil.

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Section: Resultsmentioning

confidence: 99%

Nutrient Deficiency and an Algicidal Bacterium Improved the Lipid Profiles of a Novel Promising Oleaginous Dinoflagellate, Prorocentrum donghaiense, for Biodiesel Production

Gui

Chen

Luo

et al. 2021

Appl Environ Microbiol

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“…Both APase and 5′-nucleotidase can hydrolyze PMEs, and the difference is that APase is nonspecific for a wide range of PMEs, whereas 5′-nucleotidase acts specifically on 5′-nucleotides [ 24 ]. To date, APase is probably the most well-studied phosphatase in both field studies and laboratory species studies [ 26 , 38 , 44 ]. Compared with APase, PDEase is not as well studied but is also likely important in the cycling of PDEs (DNA, RNA, cyclic nucleotides, and lipids) [ 39 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Bioavailability of Organic Phosphorus Compounds to the Harmful Dinoflagellate Karenia mikimotoi

et al. 2021

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Karenia mikimotoi is one of the most well-known harmful bloom species in temperate coastal waters. The present study investigated the characteristics of alkaline phosphatase (APase) and phosphodiesterase (PDEase) activities in hydrolysis of two phosphomonoesters (adenosine triphosphate (ATP) and ribulose 5-phosphate (R5P)) and a phosphodiester (cyclic adenosine monophosphate (cAMP)) in K. mikimotoi and compared its growth and physiological responses to the different forms of phosphorus substrates. K. mikimotoi produced comparable quantities of APase and PDEase to hydrolyze the organic phosphorus substrates, although hydrolysis of the phosphomonoesters was much faster than that of the phosphodiester. The growth of K. mikimotoi on organic phosphorus substrates was comparable to or better than that on inorganic phosphate. The difference in particulate organic nutrients (carbon, nitrogen, and phosphorus) and hemolytic activity supported different rates of hydrolysis-assimilation of the various organic phosphorus substrates by K. mikimotoi. The hemolytic activities of K. mikimotoi in the presence of organic phosphorus substrates were several times those in the presence of inorganic phosphate during the exponential phase. This suggested the potential important role of organic phosphorus in K. mikimotoi blooms.

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“…The species capable of using DOP can not only better sustain their populations in interspecific competition but can also outgrow others to form harmful algal blooms. DOP has been regarded as one of the most important nutrient sources responsible for the large blooms of Prorocentrum donghaiense in the East China Sea (Ou et al ., ). Therefore, understanding DOP utilization efficiency and regulation mechanisms is an important research topic in phytoplankton ecology (Wang et al ., ; Li et al ., ; Diaz et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization

Zhang

Lin

Chen

2020

Environmental Microbiology

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The capacity of phytoplankton to utilize dissolved organic phosphorus (DOP) plays an important role in their competition for resources when the availability of dissolved inorganic phosphorus (DIP) is low in the aquatic systems. Here, we explored the physiological and molecular responses of a globally distributed marine diatom, Skeletonema costatum, in utilizing adenosine-5 0 -triphosphate (ATP) based on incubation experiments under ATP, DIP-replete, and DIPdepleted conditions. The results show that ATP supports the growth of S. costatum as efficiently as DIP. The pathway of S. costatum involved in utilizing ATP is not related to alkaline phosphatase (AP), an important DOP hydrolase, although extracellular hydrolysis is involved. The transcriptome analysis revealed several transcripts related to the hydrolase activity (e.g. NAD+ diphosphatase), which were significantly upregulated in the ATP culture group, indicating their possible involvement in ATP hydrolysis. Meanwhile, ATP-grown S. costatum exhibited downregulation of the genes related to a series of metabolic activities (e.g. purine metabolism), apparently to adapt to ATP condition.

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Alkaline phosphatase activities and regulation in three harmful Prorocentrum species from the coastal waters of the East China Sea

Cited by 29 publications

References 60 publications

Nutrient Deficiency and an Algicidal Bacterium Improved the Lipid Profiles of a Novel Promising Oleaginous Dinoflagellate, Prorocentrum donghaiense, for Biodiesel Production

Nutrient Deficiency and an Algicidal Bacterium Improved the Lipid Profiles of a Novel Promising Oleaginous Dinoflagellate, Prorocentrum donghaiense, for Biodiesel Production

Bioavailability of Organic Phosphorus Compounds to the Harmful Dinoflagellate Karenia mikimotoi

Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization

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