Alkaline extraction and characterisation of heteroxylans from maize bran

Chanliaud, Elisabeth; Saulnier, Luc; Thibault, J.-F.

doi:10.1016/0733-5210(95)90035-7

Cited by 125 publications

(77 citation statements)

References 19 publications

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“…Chanliaud and associates 23 ob-Ç 30% of the pectins were removed after acidic served similar results with maize bran, although (B-ac) or alkaline (B-alk) extraction, and 60% the yields of extraction were much higher. with alkaline peroxide treatment (B-ox).…”

Section: Cellulose-enriched Sugar-beet Pulp Cellulose-enriched Wheat supporting

confidence: 79%

Preliminary study of formation of films from cellulose‐enriched agricultural by‐products

Chauvelon¹,

Renard²,

Saulnier³

et al. 1998

J of Applied Polymer Sci

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ABSTRACT:Two agricultural by-products, sugar-beet pulp and wheat bran, have been examined for their suitability to be transformed into bioplastics by esterification by lauroyl chloride. Influence of cellulose content was studied on eleven samples enriched in cellulose after chemical or enzymatic removal of pectins from sugar-beet pulp and heteroxylans from wheat bran. After a pretreatment by immersion in 0.5 mol/L sulfuric acid, esterification was carried out with lauroyl chloride. Neither the amount of cellulose nor the extraction treatment had a marked influence on the formation of plastic. A film could be obtained from all the wheat-bran samples, including samples with low cellulose content, but only from one sugar-beet pulp sample. The crystallinity of the cellulose in sugar-beet pulp and wheat bran were different. The nature of cellulose could be responsible for the failure of sugar-beet pulp residues to form plastic.

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Section: Cellulose-enriched Sugar-beet Pulp Cellulose-enriched Wheat supporting

confidence: 79%

Preliminary study of formation of films from cellulose‐enriched agricultural by‐products

Chauvelon¹,

Renard²,

Saulnier³

et al. 1998

J of Applied Polymer Sci

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“…In the present assay, the totality of FA from WB was released by the bifunctional enzyme treatment, and less than 8% was obtained with CB. Although ferulic acid content in corn bran is higher than that found in the WB, CB xylan is more often substituted by xylose, arabinose, and galactose residues (7,15). Thus, the difference in ferulic acid release could be explained by the number of substitutions on the heteroxylan backbone in corn, the presence of highly branched xylose in the side chain, and the presence of a linkage between arabinose and xylose at the proximity of the FA group, which seriously restrict enzyme accessibility.…”

Section: Discussionmentioning

confidence: 93%

Construction of Engineered Bifunctional Enzymes and Their Overproduction in Aspergillus niger for Improved Enzymatic Tools To Degrade Agricultural By-Products

Levasseur¹,

Navarro²,

Punt

et al. 2005

Appl Environ Microbiol

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Two chimeric enzymes, FLX and FLXLC, were designed and successfully overproduced in Aspergillus niger. FLX construct is composed of the sequences encoding the feruloyl esterase A (FAEA) fused to the endoxylanase B (XYNB) of A. niger. A C-terminal carbohydrate-binding module (CBM family 1) was grafted to FLX, generating the second hybrid enzyme, FLXLC. Between each partner, a hyperglycosylated linker was included to stabilize the constructs. Hybrid proteins were purified to homogeneity, and molecular masses were estimated to be 72 and 97 kDa for FLX and FLXLC, respectively. Integrity of hybrid enzymes was checked by immunodetection that showed a single form by using antibodies raised against FAEA and polyhistidine tag. Physicochemical properties of each catalytic module of the bifunctional enzymes corresponded to those of the free enzymes. In addition, we verified that FLXLC exhibited an affinity for microcrystalline cellulose (Avicel) with binding parameters corresponding to a K d of 9.9 ؋ 10 ؊8 M for the dissociation constant and 0.98 mol/g Avicel for the binding capacity. Both bifunctional enzymes were investigated for their capacity to release ferulic acid from natural substrates: corn and wheat brans. Compared to free enzymes FAEA and XYNB, a higher synergistic effect was obtained by using FLX and FLXLC for both substrates. Moreover, the release of ferulic acid from corn bran was increased by using FLXLC rather than FLX. This result confirms a positive role of the CBM. In conclusion, these results demonstrated that the fusion of naturally free cell wall hydrolases and an A. niger-derived CBM onto bifunctional enzymes enables the increase of the synergistic effect on the degradation of complex substrates.

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“…During the last years, much attention has been paid to develop a convenient isolation process of the corn bran heteroxylan [10][11][12]109) . Mainly, destrached bran was used as the starting material and aqueous solutions of NaOH, KOH, Ca(OH) 2 at temperatures up to 90 8C (Tab.…”

Section: Some Comments On Isolation Of Xylans From Natural Resourcesmentioning

confidence: 99%

“…Up to now, only few products are commercialized. The highly branched heteroxylan from corn hulls, a by-product of starch production, which was close to enter the market as a new food gum many years ago 3) , is nowadays under reinvestigation [10][11][12] . Sulfated xylans are used as a biologically active component in drugs 9) .…”

Section: Introductionmentioning

confidence: 99%

Xylan and xylan derivatives - biopolymers with valuable properties, 1. Naturally occurring xylans structures, isolation procedures and properties

Ebringerová¹,

Heinze

2000

Macromol. Rapid Commun.

782

373

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Alkaline extraction and characterisation of heteroxylans from maize bran

Cited by 125 publications

References 19 publications

Preliminary study of formation of films from cellulose‐enriched agricultural by‐products

Preliminary study of formation of films from cellulose‐enriched agricultural by‐products

Construction of Engineered Bifunctional Enzymes and Their Overproduction in Aspergillus niger for Improved Enzymatic Tools To Degrade Agricultural By-Products

Xylan and xylan derivatives - biopolymers with valuable properties, 1. Naturally occurring xylans structures, isolation procedures and properties

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