Alkali-stable GH11 endo-β-1,4 xylanase (XynB) from <i>Bacillus subtilis</i> strain CAM 21: application in hydrolysis of agro-industrial wastes, fruit/vegetable peels and weeds

Monica, P; Kapoor, M.

doi:10.1080/10826068.2020.1830416

Cited by 8 publications

(1 citation statement)

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“…Hemicellulose, the second most abundant lignocellulose component, can be hydrolyzed to monosaccharides by a variety of enzymatic systems (Tang et al 2021 ). The catalytic site of endo-1,4-β-xylanase from the GH11 family is the β-1,4-xylosidic linkages of xylan, producing short xylooligosaccharides after xylan cleavage (Monica and Kapoor 2021 ). In the field of pulp bleaching, xylanases from the GH11 family are favored over xylanases from the GH10 family.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic and transcriptome analysis of Bacillus velezensis CL-4 fermented corn germ meal

Chen

et al. 2023

AMB Expr

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Bacillus, an excellent organic-degrading agent, can degrade lignocellulose. Notably, some B. velezensis strains encode lignocellulases. However, their ability to degrade lignocellulose in fermented feed is not much appreciated. This study performed a comparative genomic analysis of twenty-three B. velezensis strains to find common carbohydrate-active enzymes (CAZymes) encoding genes and evaluated their potential to degrade lignocellulose. The comparative genomic and CAZyme database-based analyses identified several potential CAZymes genes that degrade cellulose (GH1, GH4, GH5, GH13, GH16, GH32, PL1, and PL9), hemicellulose (GH11, GH26, GH43, GH51, and CE3) and lignin (AA4, AA6, AA7, and AA10). Furthermore, Illumina RNA-seq transcriptome analysis revealed the expression of more than 1794 genes in B. velezensis CL-4 fermented corn germ meal at 48 h (FCGM 48 h). Gene ontology analysis of expressed genes revealed their enrichment in hydrolase activity (breaking the glycosyl bonds during carbohydrate metabolism), indicating the upregulation of CAZymes. In total, 58 differentially upregulated CAZymes-encoding genes were identified in FCGM 48 h compared to FCGM 0 h. The upregulated CAZymes-encoding genes were related to cellulose (6-phospho-β-galactosidase and 6-phospho-α-glucosidase), starch (α-glucosidase and α-amylase), pectin (pectin lyase), and hemicellulose (arabinan endo-1,5-α-L-arabinosidase, xylan 1,4-beta-xylosidase, α-N-arabinofuranosidase, and acetyl xylan esterase). Importantly, arabinoxylan degradation mainly occurred in FCGM 48 h, followed by partial degradation of cellulose, pectin, and starch. This study can support the development of enzymatic cocktails for the solid-state fermented feed (SFF).

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Section: Discussionmentioning

confidence: 99%