High-level production of xylose from agricultural wastes using GH11 endo-xylanase and GH43 β-xylosidase from Bacillus sp.

Wang, Fenghua; Yao, Zhiming; Zhang, Xue; Han, Zhuoxuan; Chu, Xiuxiu; Ge, Xiuqi; Lu, Fuping; Liu, Yihan

doi:10.1007/s00449-022-02778-w

Cited by 3 publications

(3 citation statements)

References 58 publications

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“…The synergistic catalysis mixtures comprising 200 U/g XynG1-1 and 200 U/g rXYLO in 50 mM sodium phosphate buffer (pH 8.0) with 5 g/L substrate were incubated at 50 °C and 180 rpm. The reaction was terminated by boiling for 10 min, and the xylose content was determined by high-performance liquid chromatography (Agilent Technologies, Germany) using a differential refractive index detector (RID) and Burroughs Aminex HPX-87H chromatographic column (300 × 7.8 mm, Bio-Rad, Richmond, CA) . All experiments were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

“…The reaction was terminated by boiling for 10 min, and the xylose content was determined by high-performance liquid chromatography (Agilent Technologies, Germany) using a differential refractive index detector (RID) and Burroughs Aminex HPX-87H chromatographic column (300 × 7.8 mm, Bio-Rad, Richmond, CA). 40 All experiments were performed in triplicate.…”

Section: Biocatalytic Hydrolysis Of Corn Stover Xylan For the Product...mentioning

confidence: 99%

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Computation-Aided Phylogeny-Oriented Engineering of β-Xylosidase: Modification of “Blades” to Enhance Stability and Activity for the Bioconversion of Hemicellulose to Produce Xylose

Zhang,

Gao,

Song

et al. 2024

J. Agric. Food Chem.

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Hemicellulose is a highly abundant, ubiquitous, and renewable natural polysaccharide, widely present in agricultural and forestry residues. The enzymatic hydrolysis of hemicellulose has generally been accomplished using β-xylosidases, but concomitantly increasing the stability and activity of these enzymes remains challenging. Here, we rationally engineered a βxylosidase from Bacillus clausii to enhance its stability by computation-aided design combining ancestral sequence reconstruction and structural analysis. The resulting combinatorial mutant rXYLO M25I/S51L/S79E exhibited highly improved robustness, with a 6.9-fold increase of the half-life at 60 °C, while also exhibiting improved pH stability, catalytic efficiency, and hydrolytic activity. Structural analysis demonstrated that additional interactions among the propeller blades in the catalytic module resulted in a much more compact protein structure and induced the rearrangement of the opposing catalytic pocket to mediate the observed improvement of activity. Our work provides a robust biocatalyst for the hydrolysis of agricultural waste to produce various high-value-added chemicals and biofuels.

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Section: Methodsmentioning

confidence: 99%

Section: Biocatalytic Hydrolysis Of Corn Stover Xylan For the Product...mentioning

confidence: 99%

Computation-Aided Phylogeny-Oriented Engineering of β-Xylosidase: Modification of “Blades” to Enhance Stability and Activity for the Bioconversion of Hemicellulose to Produce Xylose

Zhang,

Gao,

Song

et al. 2024

J. Agric. Food Chem.

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“…Small molecules like mono, di, and tri-saccharides of D-xylopyranosyl may be produced at a later stage of hydrolysis after the synthesis of D-xylopyranosyl oligomers. After the xylan has been sufficiently broken down into short oligomers, xylosidases are activated [ 109 ]. The aggregation of such oligomers may hinder the action of endoxylanase, and therefore as a result, β-xylosidase hydrolyzes these oligomers into xylose monomer, minimizing the inhibitory effects of oligomers and increasing xylan hydrolysis efficiency [ 82 ].…”

Section: Enzymatic Hydrolysis Of Xylan For Xylooligosaccharides Produ...mentioning

confidence: 99%

Bioprocess development for the production of xylooligosaccharide prebiotics from agro-industrial lignocellulosic waste

Dong

Tsai

Nargotra

et al. 2023

Heliyon

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Diversity and saline-alkali resistance of Coleoptera endosymbiont bacteria in arid and semi-arid climate

Yue,

Ma,

Sun

et al. 2024

Microbiol Spectr

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Soil salinization usually occurs in arid and semi-arid climate areas from 37 to 50 degrees north latitude and 73 to 123 degrees east longitude. These regions are inhabited by a large number of Coleopteran insects, which play an important role in the ecological cycle. However, little is known about the endosymbiotic microbial taxa and their biological characteristics in these insects. A study of endosymbiotic microorganisms of Coleoptera from Xinjiang, a typical arid and inland saline area, revealed that endosymbiont bacteria with salinity tolerance are common among the endosymbionts of Coleoptera. Functional prediction of the microbiota analysis indicated a higher abundance of inorganic ion transporters and metabolism in these endosymbiont strains. Screening was conducted on the tolerable 11% NaCl levels of Brevibacterium casei G20 ( PRJNA754761 ), and differential metabolite and proteins were performed. The differential metabolites of the strain during the exponential and plateau phases were found to include benzene compounds, organic acids, and their derivatives. These results suggest that the endosymbiotic microorganisms of Coleoptera in this environment have adaptive evolution to extreme environments, and this group of microorganisms is also one of the important resources for mining saline and alkaline-tolerant chassis microorganisms and high-robustness enzymes. IMPORTANCE Coleoptera insects, as the first largest order of insect class, have the characteristics of a wide variety and wide distribution. The arid and semi-arid climate makes it more adaptable. By studying the endosymbiont bacteria of Coleoptera insects, we can systematically understand the adaptability of endosymbiont bacteria to host and special environment. Through the analysis of endosymbiont bacteria of Coleoptera insects in different saline-alkali areas in arid and semi-arid regions of Xinjiang, it was found that bacteria in different host samples were resistant to saline-alkali stress. These results suggest that bacteria and their hosts co-evolved in response to this climate. Therefore, this study is of great significance for understanding the endosymbiont bacteria of Coleoptera insects and obtaining extremophile resources (Saline-alkali-resistant chassis strains with modification potential for the production of bulk chemicals and highly robust industrial enzymes).

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High-level production of xylose from agricultural wastes using GH11 endo-xylanase and GH43 β-xylosidase from Bacillus sp.

Cited by 3 publications

References 58 publications

Computation-Aided Phylogeny-Oriented Engineering of β-Xylosidase: Modification of “Blades” to Enhance Stability and Activity for the Bioconversion of Hemicellulose to Produce Xylose

Computation-Aided Phylogeny-Oriented Engineering of β-Xylosidase: Modification of “Blades” to Enhance Stability and Activity for the Bioconversion of Hemicellulose to Produce Xylose

Bioprocess development for the production of xylooligosaccharide prebiotics from agro-industrial lignocellulosic waste

Diversity and saline-alkali resistance of Coleoptera endosymbiont bacteria in arid and semi-arid climate

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