Alienimonas californiensis gen. nov. sp. nov., a novel Planctomycete isolated from the kelp forest in Monterey Bay

Abstract

Planctomycetes are environmentally and biotechnologically important bacteria and are often found in association with nutrient-rich (marine) surfaces. To allow a more comprehensive understanding of planctomycetal lifestyle and physiology we aimed at expanding the collection of axenic cultures with new isolates. Here, we describe the isolation and genomic and physiological characterisation of strain CA12 T obtained from giant bladder kelp (Macrocystis pyrifera) in Monterey Bay, California, USA. 16S rRNA gene sequence and whole genome-based phylogenetic analysis showed that strain CA12 T clusters within the family Planctomycetaceae and that it has a high 16S rRNA sequence similarity (82.3%) to Planctomicrobium piriforme DSM 26348 T . The genome of strain CA12 T has a length of 5,475,215 bp and a G?C content of 70.1%. The highest growth rates were observed at 27 °C and pH 7.5. Using different microscopic methods, we could show that CA12 T is able to divide by consecutive polar budding, without completing a characteristic planctomycetal lifestyle switch. Based on our data, we suggest that the isolated strain represents a novel species within a novel genus. We thus propose the name Alienimonas gen. nov. with Alienimonas californiensis sp. nov. as type species of the novel genus and CA12 T as type strain of the novel species.

Description of Polystyrenella longa gen. nov., sp. nov., isolated from polystyrene particles incubated in the Baltic Sea

“…Field emission scanning electron microscopy was performed as described (Boersma et al 2019). Briefly, bacteria were fixed in formaldehyde, washed, and placed on cover slips coated with poly-L-lysine solution.…”

“…16S rRNA gene-based phylogeny was computed for strain Pla110 T , the type strains of all described planctomycetal species (assessed in January 2020), all isolates recently described (Boersma et al 2019;Kallscheuer et al 2019aKallscheuer et al , b, c, d, 2020Kohn et al 2019;Peeters et al 2019;Rensink et al 2020;) and with an outgroup of strains from outside the Planctomycetes, but part of the PVC superphylum. The alignment of 16S rRNA genes was made with SINA (Pruesse et al 2012).…”

Lignipirellula cremea gen. nov., sp. nov., a planctomycete isolated from wood particles in a brackish river estuary

“…Field emission scanning electron microscopy was performed as described previously (Boersma et al 2019). The bacteria were fixed in formaldehyde, washed and placed on poly-L-lysine-coated cover slips.…”

“…16S rRNA gene-based phylogeny was computed for strain Pla85_3_4 T , the type strains of all described planctomycetal species (assessed in January 2020), all isolates recently published (Boersma et al 2019;Kallscheuer et al 2019aKallscheuer et al , b, c, d, 2020Kohn et al 2019;Peeters et al 2019;Rensink et al 2020) and with an outgroup of strains from outside the phylum Planctomycetes but part of the PVC superphylum. The alignment of 16S rRNA genes was made with SINA (Pruesse et al 2012).…”

Aureliella helgolandensis gen. nov., sp. nov., a novel Planctomycete isolated from a jellyfish at the shore of the island Helgoland

“…Species belonging to the class Planctomycetia have been isolated from various marine biotic and abiotic surfaces (Boersma et al 2019;Bondoso et al 2014Bondoso et al , 2017Kallscheuer et al 2020;Peeters et al 2020;Vollmers et al 2017), on which they can be highly abundant (Bengtsson and Øvreås 2010). Due to the oligotrophic nature of marine environments, such species are suggested to digest complex carbon substrates, e.g.…”