Algorithm for the determination of a linear GPC calibration curve using a polydisperse standard

Malawer, E. G.; Montana, Anthony J.

doi:10.1002/pol.1980.180181116

Cited by 10 publications

(5 citation statements)

References 8 publications

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“…(Polymerization with constant helium purging of an 8%T in water resulted in a solution too viscous to handle in the dilution step.) In order to characterize this polymer, a molecular weight standard with M w = 5.5 × 10 6 Da was used to calibrate the SEC column with the Hamilec approach . Unfortunately, although the HPLC column provided the highest exclusion limit available, an accurate calibration of the column could not be achieved.…”

Section: Resultsmentioning

confidence: 99%

Rapid DNA Sequencing of More Than 1000 Bases per Run by Capillary Electrophoresis Using Replaceable Linear Polyacrylamide Solutions

et al. 1996

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The read length for DNA sequencing using capillary electrophoresis and replaceable linear polyacrylamide (LPA) solutions has been extended to more than 1000 bases with a run time of 80 min. This result was successfully achieved through the combined use of cycle sequencing with dye-labeled primers, improved matrix and separation conditions, and enhanced base-calling software. The influences of LPA molecular weight and concentration on separation were investigated. Additionally, the separation buffer, column temperature, and electric field were adjusted to increase the number of resolvable DNA fragments per run while maintaining an enhanced separation speed. Using low concentrations [2% (w/v)] of high molecular weight LPA polymers (> 5.5 x 10(6) Da), elevated column temperature (50 degrees C) and moderately high field (150 V/cm), rapid sequencing analysis for more than 1000 bases on a model ssM13mp18 template was obtained with 96.8% accuracy.

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Section: Resultsmentioning

confidence: 99%

Rapid DNA Sequencing of More Than 1000 Bases per Run by Capillary Electrophoresis Using Replaceable Linear Polyacrylamide Solutions

et al. 1996

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“…The second was done by internal calibration based on sedimentation–diffusion analysis data, and the third one was made also with sedimentation–diffusion analysis data with implementation of procedures developed for optimized internal calibration with polydisperse standards (Table S1). , …”

Section: Methodsmentioning

confidence: 99%

Diels–Alder Hyperbranched Pyridylphenylene Polymer Fractions as Alternatives to Dendrimers

Gubarev¹,

Лезов²,

Senchukova³

et al. 2019

Macromolecules

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The results of the hydrodynamic study of hyperbranched pyridylphenylene polymers synthesized via Diels–Alder cycloaddition reaction of multifunctional monomers are reported. The synthesized polymers were fractionated into a much narrowly dispersed fractions. The in-depth analysis of molecular characteristics and conformation of the initial polymers and corresponding fractions was performed using the unique combination of hydrodynamic approaches. The intercorrelation and self-consistency of obtained data were ensured using the hydrodynamic invariant concept and classical Kuhn–Mark–Houwink–Sakurada scaling relations. The evaluated data suggest that hyperbranched pyridylphenylene demonstrates the hydrodynamic behavior typical for asymmetrical compact macromolecular species. Furthermore, hydrodynamic characteristics of herein studied hyperbranched pyridylphenylene polymer fractions were compared with known hydrodynamic data for pyridylphenylene dendrimers. Nearly identical hydrodynamic behavior was found for the compared macromolecules, especially for low molecular mass fractions, which may suggest that the fractionation of hyperbranched polymers results in dendrimer-like structures. The oblate ellipsoid model provides reasonable correlation between hydrodynamic properties of hyperbranched pyridylphenylene polymer fractions and pyridylphenylene dendrimers.

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“…In organischen L6sungsmitteln kann ein Zusammenhang zwischen Elutionsvolumen und der Molmasse tiber eine Eichung mit engverteilten Polymerstandards durchgeftihrt werden [17][18][19][20]. Dieses Verfahren bringt bei w~igrigen Systemen Probleme, da keine geeigneten Standards zur Verfiigung stehen.…”

Section: Introductionunclassified

Bestimmung der Molmassenverteilung sowie der Stabilit�tsgrenze von Polyacrylamiden unter Benutzung einer kombinierten Ausschlu�chromatographie/Kleinwinkel-Laser-Streulichtphotometer Anlage

Kulicke

Böse

1984

Colloid & Polymer Sci

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Verzeichnis derThe molecular weight distribution including the calculation of M n, M~ and M z of polyacrylamide in aqueous 0.1 M sodium sulphate-solution has been determined by simultaneously and continuously monitoring the effluent from a size-exclusion chromatographic (SEC) column filled with porous glasses with a low-angle-laser-light-scattering (LALLS) photometer. The recorder traces of the scattering intensity and the refractive index difference have been digitalized in about 400 steps corresponding to 200 #1 fractions. Each fraction has been assumed to be monodisperse. This method gives absolute data rather than relative data obtained from any calibration method. The investigations clearly show a flow-rate dependent degradation of samples with relatively low molar masses (M~ = 500 000 g/mol at MJM n = 2.5) at typical flow rates Q -> 1 ml/min. A discussion of some problems encountered using aqueous SEC and a way to determine the critical molar mass, below which no degradation could be observed, based on elongational flow theories is given.

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Algorithm for the determination of a linear GPC calibration curve using a polydisperse standard

Cited by 10 publications

References 8 publications

Rapid DNA Sequencing of More Than 1000 Bases per Run by Capillary Electrophoresis Using Replaceable Linear Polyacrylamide Solutions

Rapid DNA Sequencing of More Than 1000 Bases per Run by Capillary Electrophoresis Using Replaceable Linear Polyacrylamide Solutions

Diels–Alder Hyperbranched Pyridylphenylene Polymer Fractions as Alternatives to Dendrimers

Bestimmung der Molmassenverteilung sowie der Stabilit�tsgrenze von Polyacrylamiden unter Benutzung einer kombinierten Ausschlu�chromatographie/Kleinwinkel-Laser-Streulichtphotometer Anlage

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