Throughput of dynamic frame slotted ALOHA (DFSA) in radio frequency identification (RFID) systems depends on the tags quantity estimate. This paper shows how to apply the slot-by-slot (SbS) estimate approach, along with the policy for the early frame-break. Simulation results show noticeable throughput improvements.Note to Practitioners-RFID supply chain application relies on the fast tag identification. As already shown in the literature, maximizing the number of read tags in the unit of time is a challenging task. In this paper, we propose a method which can assure near optimal tag identification time while maintaining low computational burden at the implementation point of view.Index Terms-Dynamic frame slotted ALOHA (DFSA), early frame break, efficient tag estimate method (TEM), slot-by-slot (SbS) estimation.