Alginate modified-PLGA nanoparticles entrapping amikacin and moxifloxacin as a novel host-directed therapy for multidrug-resistant tuberculosis

Abdelghany, Sharif; Parumasivam, Thaigarajan; Pang, Angel; Roediger, Ben; Tang, Patricia A.; Jahn, Kristina A.; Britton, Warwick J.; Chan, Hak‐Kim

doi:10.1016/j.jddst.2019.05.025

Cited by 64 publications

(30 citation statements)

References 50 publications

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“…No clear peak presented (Figure 5h–m) in the thermograms of the prepared uncoated and CS-coated PLGA NPs due to any possible decrease in the drug crystallinity and/or solvation of the drug in the melted carrier and/or heat-induced interaction between drug and polymer [47]. This disappearance of melting peaks of CLR from the NP formulation thermograms indicated that CLR was likely encapsulated in the amorphous state and molecularly dispersed in the polymeric structure [48,49]. The thermograms demonstrated that there was no interaction between the CLR and the polymers.…”

Section: Resultsmentioning

confidence: 99%

Clarithromycin-Loaded Poly (Lactic-co-glycolic Acid) (PLGA) Nanoparticles for Oral Administration: Effect of Polymer Molecular Weight and Surface Modification with Chitosan on Formulation, Nanoparticle Characterization and Antibacterial Effects

2019

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Clarithromycin (CLR) is a member of the macrolide antibiotic group. CLR has low systemic oral bioavailability and is a drug of class II of the Biopharmaceutical Classification System. In many studies, using nanoparticles (NPs) as a drug delivery system has been shown to increase the effectiveness and bioavailability of active drug substances. This study describes the development and evaluation of poly (lactic-co-glycolic acid) (PLGA) NPs and chitosan (CS)-coated PLGA NPs for oral delivery of CLR. NPs were obtained by nanoprecipitation technique and characterized in detail, and the effect of three molecular weights (Mw1: 7.000–17.000, Mw2: 38.000–54.000, Mw3: 50.000–190.000) of PLGA and CS coating on particle size (PS), zeta potential (ZP), entrapment efficiency (EE%), and release properties etc. were elucidated. Gastrointestinal stability and cryoprotectant effect tests were performed on the NPs. The PS of the prepared NPs were in the range of 178 to 578 nm and they were affected by the Mw and CS coating. In surface-modified formulations with CS, the ZP of the NPs increased significantly to positive values. EE% varied from 62% to 85%, depending upon the Mw and CS coating. In vitro release studies of CLR-loaded NPs showed an extended release up to 144 h. Peppas–Sahlin and Weibull kinetic model was found to fit best for CLR release from NPs. By the broth microdilution test method, the antibacterial activity of the formulations was determined on Staphylococcus aureus (ATCC 25923), Listeria monocytogenes (ATCC 1911), and Klebsiella pneumoniae (ATCC 700603). The structures of the formulations were clarified by thermal (DSC), FT-IR, and 1H-NMR analysis. The results showed that PS, ZP, EE%, and dissolution rates of NPs were directly related to the Mw of PLGA and CS coating.

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Section: Resultsmentioning

confidence: 99%

Clarithromycin-Loaded Poly (Lactic-co-glycolic Acid) (PLGA) Nanoparticles for Oral Administration: Effect of Polymer Molecular Weight and Surface Modification with Chitosan on Formulation, Nanoparticle Characterization and Antibacterial Effects

2019

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“…They are also able to improve cellular uptake [12]. Polymeric nanoparticles also provide a sustained release system and improve the stability of labile drugs from in vivo enzymatic degradation [13]. Presently, a variety of natural and synthetic biodegradable polymer-based nanoparticles can be used to enhance immunogenicity for vaccine delivery.…”

Section: Polymer-based Nanoparticlesmentioning

confidence: 99%

Applications of polymer-based nanoparticles in vaccine field

Guo

Utupova

et al. 2019

Nanotechnology Reviews

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Polymer-based nanoparticles have good solubility, stability, safety, and sustained release,which increases the absorption of loaded drugs, protects the drugs from degradation, and prolongs their circulation time and targeted delivery. Generally, we believe that prevention and control of infectious diseases through inoculation is the most efficient measure. However, these vaccines including live attenuated vaccines, inactivated vaccines, protein subunit vaccines, recombinant subunit vaccines, synthetic peptide vaccines and DNA vaccines have several defects, such as immune tolerance, poor immunogenicity, low expression level and induction of respiration pathological changes. All kinds of biodegradable natural and synthetic polymers play major roles in the vaccine delivery system to control the release of antigens for an extended period of time. In addition, these polymers also serve as adjuvants to enhance the immunogenicity of vaccine. This review mainly introduces natural and synthetic polymer-based nanoparticles and their formulation and properties. Moreover, polymer-based nanoparticles as adjuvants and delivery carriers in the applications of vaccine are also discussed. This review provides the basis for further operation of nano vaccines by utilizing the polymer-based nanoparticles as vaccine adjuvants and delivery systems. Polymer-based nanoparticles have exhibited great potential in improving the immunogenicity of antigens and the development of nano vaccines in future.

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“…In consequence, controlled release systems based on pectin or alginate often exhibit higher swelling degree and water penetration, with the consequent enhancement of drug release 12 . The combination of pectin or alginate with hydrophobic polymers has been studied in order to obtain nanoparticles, 13 microparticles 1,14,15 and films 16,17 by different methods. Particularly, biomaterials have been added to the W1 phase or suspended in O phase of the emulsion technique during PLGA microparticles preparation for the entrapment of antibiotics 13 and proteins 18–21 .…”

Section: Introductionmentioning

confidence: 99%

“…The combination of pectin or alginate with hydrophobic polymers has been studied in order to obtain nanoparticles, 13 microparticles 1,14,15 and films 16,17 by different methods. Particularly, biomaterials have been added to the W1 phase or suspended in O phase of the emulsion technique during PLGA microparticles preparation for the entrapment of antibiotics 13 and proteins 18–21 . Thus, the combination of PLGA with pH‐responsive biopolymers offers new opportunities for the controlled release of active principles.…”

Section: Introductionmentioning

confidence: 99%

Modulating drug release from poly(lactic‐co‐glycolic) acid microparticles by the addition of alginate and pectin

Karp

Satler

Busatto

et al. 2020

J of Applied Polymer Sci

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The aim of the present work is the characterization of PLGA microparticles including biopolymers for the controlled release of tilmicosin, a broad‐spectrum antibiotic. Microparticles were prepared using the double‐emulsion solvent evaporation technique. The effect of alginate and pectin incorporation over particle size and porosity, encapsulation efficiency (EE) and pH‐responsive drug release was evaluated. Formulations presented a mean particle size of 5.5 μm approximately and a drug EE ranged from 22%–57%. PLGA‐Alginate particles showed an increased porosity. Tilmicosin release profiles from PLGA and PLGA‐biopolymer microparticles were affected by the particular combination of polymers and the pH of the release medium. The experimental data was simulated using a mathematical model, which takes into account the autocatalytic polymer degradation and the different mechanisms of drug transport. The combination of PLGA and biopolymers strongly influenced the morphology of the particles, offering the possibility of controlling the drug release profiles according to the therapy.

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Alginate modified-PLGA nanoparticles entrapping amikacin and moxifloxacin as a novel host-directed therapy for multidrug-resistant tuberculosis

Cited by 64 publications

References 50 publications

Clarithromycin-Loaded Poly (Lactic-co-glycolic Acid) (PLGA) Nanoparticles for Oral Administration: Effect of Polymer Molecular Weight and Surface Modification with Chitosan on Formulation, Nanoparticle Characterization and Antibacterial Effects

Clarithromycin-Loaded Poly (Lactic-co-glycolic Acid) (PLGA) Nanoparticles for Oral Administration: Effect of Polymer Molecular Weight and Surface Modification with Chitosan on Formulation, Nanoparticle Characterization and Antibacterial Effects

Applications of polymer-based nanoparticles in vaccine field

Modulating drug release from poly(lactic‐co‐glycolic) acid microparticles by the addition of alginate and pectin

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