Alginate immobilization as a strategy for improving succinate production during autofermentation using cyanobacteria Synechocystis sp. PCC 6803

Alfaro-Sayes, Daniel A; Amoah, Jerome; Aikawa, Shimpei; Matsuda, Mami; Hasunuma, Tomohisa; Kondo, Akihiko; Ogino, Chiaki

doi:10.1016/j.bej.2022.108681

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…It was demonstrated in other works that the immobilization of Synechocystis is an effective way to increase the production yield of the cells. Immobilization in Ca 2+ -alginate beads was reported to increase succinate [ 26 ] and β -phellandrene [ 27 ] production, while immobilization in Ca 2+ -alginate thin film was shown to be effective for the increase of ethylene production [ 28 ]. The immobilization was previously reported to be effective to increase sucrose production as well, with engineered Synechococcus elongatus sp.…”

Section: Resultsmentioning

confidence: 99%

Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli

Tóth

Siitonen

Nikkanen

et al. 2022

Biotechnol Biofuels

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Background Whole-cell biotransformation is a promising emerging technology for the production of chemicals. When using heterotrophic organisms such as E. coli and yeast as biocatalysts, the dependence on organic carbon source impairs the sustainability and economic viability of the process. As a promising alternative, photosynthetic cyanobacteria with low nutrient requirements and versatile metabolism, could offer a sustainable platform for the heterologous production of organic compounds directly from sunlight and CO2. This strategy has been applied for the photoautotrophic production of sucrose by a genetically engineered cyanobacterium, Synechocystis sp. PCC 6803 strain S02. As the key concept in the current work, this can be further used to generate organic carbon compounds for different heterotrophic applications, including for the whole-cell biotransformation by yeast and bacteria. Results Entrapment of Synechocystis S02 cells in Ca2+-cross-linked alginate hydrogel beads improves the specific sucrose productivity by 86% compared to suspension cultures during 7 days of cultivation under salt stress. The process was further prolonged by periodically changing the medium in the vials for up to 17 days of efficient production, giving the final sucrose yield slightly above 3000 mg l−1. We successfully demonstrated that the medium enriched with photosynthetically produced sucrose by immobilized Synechocystis S02 cells supports the biotransformation of cyclohexanone to ε-caprolactone by the E. coli WΔcscR Inv:Parvi strain engineered to (i) utilize low concentrations of sucrose and (ii) perform biotransformation of cyclohexanone to ε-caprolactone. Conclusion We conclude that cell entrapment in Ca2+-alginate beads is an effective method to prolong sucrose production by the engineered cyanobacteria, while allowing efficient separation of the cells from the medium. This advantage opens up novel possibilities to create advanced autotroph–heterotroph coupled cultivation systems for solar-driven production of chemicals via biotransformation, as demonstrated in this work by utilizing the photosynthetically produced sucrose to drive the conversion of cyclohexanone to ε-caprolactone by engineered E. coli.

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Section: Resultsmentioning

confidence: 99%

Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli

Tóth

Siitonen

Nikkanen

et al. 2022

Biotechnol Biofuels

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“…This enhanced growth effect was utilized for the continuous production of succinate, a versatile biochemical compound with numerous applications. 22 While the cultivation methods involving hydrogel beads and, more recently, liquid marbles have demonstrated their efficacy, both platforms come with their respective drawbacks. For liquid marbles, due to the rapid evaporation rate of the liquid through the porous shell, a specialised setup is required to maintain the humidity and counteract excessive evaporation.…”

Section: Introductionmentioning

confidence: 99%

“…This enhanced growth effect was utilized for the continuous production of succinate, a versatile biochemical compound with numerous applications. 22 …”

Section: Introductionmentioning

confidence: 99%

Calcium alginate elastic capsules for microalgal cultivation

Tran,

Nguyen,

Yadav

et al. 2024

RSC Adv.

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“…It is biodegradable, ensuring minimal environmental impact and promoting sustainability. It can form gels with calcium ions, providing a solid matrix for trapping microorganisms and contaminants, and immobilised microorganisms in alginate matrices can be recovered and reused, increasing the efficiency and cost-effectiveness of treatment processes [20,21].…”

Section: Introductionmentioning

confidence: 99%

Immobilised Inulinase from Aspergillus niger for Fructose Syrup Production: An Optimisation Model

Lara-Fiallos,

Ayala Chamorro,

Espín-Valladares

et al. 2024

Foods

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Fructose is a carbohydrate with essential applications in the food industry, mainly due to its high sweetness and low cost. The present investigation focused on optimising fructose production from commercial inulin using the enzymatic immobilisation method and applying the response surface methodology in a 12-run central composite design. The independent variables evaluated were the pH (−) and temperature (°C). The substrate consisted of a commercial inulin solution at a concentration of 1 g/L, while the catalyst consisted of the enzyme inulinase from Aspergillus niger (EC 232-802-3), immobilised in 2% m/v sodium alginate. A stirred vessel reactor was used for 90 min at 120 rpm, and quantification of reducing sugars was determined using DNS colorimetric and UV–Vis spectrophotometric methods at a 540 nm wavelength. After applying the response surface methodology, it was determined that the catalytic activity using the immobilisation method allows for a maximum total productivity of 16.4 mg/h under pH and temperature of 3.9 and 37 °C, respectively, with an efficiency of 96.4%. The immobilised enzymes’ reusability and stability compared to free enzymes were evaluated, obtaining activity up to the fifth reuse cycle and showing significant advantages over the free catalyst.

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Alginate immobilization as a strategy for improving succinate production during autofermentation using cyanobacteria Synechocystis sp. PCC 6803

Cited by 4 publications

References 31 publications

Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli

Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli

Calcium alginate elastic capsules for microalgal cultivation

Immobilised Inulinase from Aspergillus niger for Fructose Syrup Production: An Optimisation Model

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