Alginate Films as Macromolecular Imprinted Matrices

Abstract

Macromolecularly imprinted polymers have been developed to mimic the non-covalent interactions driving molecular recognition in nature. The creation of an engineered antibody mimic would allow for the development of customizable films for biomolecular sensing. To demonstrate this principle, a cross-linked alginate film has been imprinted with bovine serum albumin (BSA) using aqueous biocompatible gelation methods. The imprinting efficiency of the synthesized films imprinted with BSA was determined and compared to the non-specific uptake of complementary proteins which were not imprinted in the alginate matrix. It was found that the recognition of the BSA using an alginate film was 6.4 mg/g polymer, which compares favorably to previously reported macromolecularly imprinted networks. The absorption of non-imprinted cationic proteins by the alginate matrix demonstrates that overcoming non-specific binding needs to be a focus of future work in order to successfully employ these materials towards biomolecular sensing within a physiological environment.

Protein molecularly imprinted polysiloxane prepared using calcium silicate/calcium alginate composite membrane as the matrix

“…Zhao等人 [13] 以CaCl 2 交联海藻酸钠和磷酸氢二铵, 制备了蛋白质印迹磷酸/ 海藻酸钙凝胶微球. Peppas等人 [14] 用延流法制备了 BSA印迹海藻酸钙膜. 宋欢语等人 [15] 硅氧烷的研究已有一些报道.…”

Molecularly Imprinted Intelligent Scaffolds for Tissue Engineering Applications

“…However, the development of more biocompatible systems for biomedical and food industry applications is the subject of continuing research. Peppas and co-workers 75,76 have developed molecular imprinting methodologies for biomedical purposes using alginate and calcium chloride. Herrero et al 76 developed a biocompatible system with the ability to achieve 3.0 mg of BSA per gram of capsules, compared to 0.7 mg achieved via inverse suspension by Zhao et al, 69 without using any chemicals besides sodium alginate and calcium chloride.…”

The challenge to improve the response of biomaterials to the physiological environment

“…Despite these challenges, protein MIPs are an active area of biomedical research because of the ultimate novelty of these ‘plastic antibodies’ [ 10 ]. Recognitive networks for cytochrome c, lysozyme [ 11 ], angiotensin II [ 12 ] and albumin [ 8 ] have been synthesized in synthetic and natural [ 13 ] hydrogel platforms. Recognition of cytochrome c is shown in Figure 4 from the recent studies of these authors.…”