Alginate-controlled formation of nanoscale calcium carbonate and hydroxyapatite mineral phase within hydrogel networks

Xie, Minli; Olderøy, Magnus Ø.; Andreassen, Jens‐Petter; Selbach, Sverre M.; Strand, Berit Løkensgard; Sikorski, Pawel

doi:10.1016/j.actbio.2010.03.034

Cited by 70 publications

(79 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently we have demonstrated a technique to form alginate microbeads that are mineralised with calcium phosphate or calcium carbonate for application in bone tissue engineering [31]. In the present study we apply the same principle to form alginate microbeads mineralised with copper containing mineral phases, in order to achieve tunable copper releasing properties.…”

Section: Introductionmentioning

confidence: 99%

Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels

Bassett¹,

Madzovska²,

Beckwith³

et al. 2014

Biomed. Mater.

Self Cite

View full text Add to dashboard Cite

Here we investigate the dissolution behaviour of copper minerals contained within biocompatible alginate hydrogels. Copper has a number of biological effects and has most recently been evaluated as an alternative to expensive and controversial growth factors for applications in tissue engineering.Precise control and sustained release of copper ions are important due to a narrow therapeutic window of this potentially toxic ion, and alginate would appear to be a good material of choice for this purpose. We found that aqueously insoluble copper minerals could be precipitated during gelling within or mixed into alginate hydrogels in the form of microbeads prior to gelling to serve as depots of copper. These minerals were found to be soluble in a variety of biological fluids relevant to in vitro and in vivo investigations, and the alginate carrier served as a barrier to diffusion of these ions and therefore offered control over the rate and duration of release (Cu 2+ release rates observed between 10 -750 µMol g -1 h -1 and duration for up to 32 days). Copper mineral and copper mineralized alginate microbeads were characterized using powder x-ray diffraction, FTIR, thermogravimetric analysis and scanning electron microscopy. Dissolution kinetics were studied to determine copper ion concentrations using colourimetric methods. In addition we characterised the complexes formed between released copper ions and biological fluids by electron paramagnetic spectroscopy which offers an insight into the behaviour of these materials in the body.

show abstract

Section: Introductionmentioning

confidence: 99%

Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels

Bassett¹,

Madzovska²,

Beckwith³

et al. 2014

Biomed. Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is well known that acidic polysaccharides are prone to forming spheroidal architectures in aqueous solution by means of calcium-mediated gelation. A typical instance is the Ca 2þ -alginate nanoparticles formed via self-assembly in aqueous environments [42][43][44][45]. In light of this, given that divalent cations, i.e.…”

Section: Nanoparticles Observed In Sundew Adhesivementioning

confidence: 99%

Sundew adhesive: a naturally occurring hydrogel

Huang

Wang

Sun

et al. 2015

J. R. Soc. Interface.

View full text Add to dashboard Cite

Bioadhesives have drawn increasing interest in recent years, owing to their ecofriendly, biocompatible and biodegradable nature. As a typical bioadhesive, sticky exudate observed on the stalked glands of sundew plants aids in the capture of insects and this viscoelastic adhesive has triggered extensive interests in revealing the implied adhesion mechanisms. Despite the significant progress that has been made, the structural traits of the sundew adhesive, especially the morphological characteristics in nanoscale, which may give rise to the viscous and elastic properties of this mucilage, remain unclear. Here, we show that the sundew adhesive is a naturally occurring hydrogel, consisting of nanonetwork architectures assembled with polysaccharides. The assembly process of the polysaccharides in this hydrogel is proposed to be driven by electrostatic interactions mediated with divalent cations. Negatively charged nanoparticles, with an average diameter of 231.9 + 14.8 nm, are also obtained from this hydrogel and these nanoparticles are presumed to exert vital roles in the assembly of the nano-networks. Further characterization via atomic force microscopy indicates that the stretching deformation of the sundew adhesive is associated with the flexibility of its fibrous architectures. It is also observed that the adhesion strength of the sundew adhesive is susceptible to low temperatures. Both elasticity and adhesion strength of the sundew adhesive reduce in response to lowering the ambient temperature. The feasibility of applying sundew adhesive for tissue engineering is subsequently explored in this study. Results show that the fibrous scaffolds obtained from sundew adhesive are capable of increasing the adhesion of multiple types of cells, including fibroblast cells and smooth muscle cells, a property that results from the enhanced adsorption of serum proteins. In addition, in light of the weak cytotoxic activity exhibited by these scaffolds towards a variety of mammal cells, evidence is sufficient to propose that sundew adhesive is a promising nanomaterial worth further exploitation in the field of tissue engineering.

show abstract

“…The presence 18 , but there may also be an interaction between strontium ions and the COO -groups of alginate. Figure 3 shows the morphological features of the ALG/ β-TCP/Sr microspheres.…”

Section: Resultsmentioning

confidence: 99%

Strontium Incorporation on Microspheres of Alginate/β-tricalcium Phosphate as Delivery Matrices

et al. 2014

View full text Add to dashboard Cite

Strontium (Sr) is known to positively affect the mechanism of bone remodelling. Consequently, calcium phosphate bioceramics associated with alginate matrices containing strontium could improve bone regeneration due to gradual strontium release. This work aims to incorporate Sr on microspheres of alginate (ALG)/β-tricalcium phosphate (β-TCP) and evaluates the in vitro release of strontium ions into a buffer solution at pH 4.0 and 7.4. In this study, strontium chloride was employed as a cross-linking agent and Sr source. Energy dispersive spectroscopy (EDS) showed that strontium was incorporated mainly at the surface of the microspheres produced. The in vitro experiments revealed that there is a rapid strontium release up to 24 h at pH 7.4 due to Sr location on microspheres' surface. At pH 4.0 both calcium and strontium were released due to the β-TCP dissolution.

show abstract

Alginate-controlled formation of nanoscale calcium carbonate and hydroxyapatite mineral phase within hydrogel networks

Cited by 70 publications

References 48 publications

Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels

Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels

Sundew adhesive: a naturally occurring hydrogel

Strontium Incorporation on Microspheres of Alginate/β-tricalcium Phosphate as Delivery Matrices

Contact Info

Product

Resources

About