Alginate cell encapsulation: new advances in reproduction and cartilage regenerative medicine

Ghidoni, I.; Chlapanidas, Theodora; Bucco, Massimo; Crovato, F.; Marazzi, Mario; Vigo, D.; Torre, Maria Luisa; Faustini, M.

doi:10.1007/s10616-008-9161-0

Cited by 94 publications

(66 citation statements)

References 64 publications

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“…Further, stem cells need to be given appropriate cues, such as signaling molecules, mechanical stiffness, or material composition to induce proper cell differentiation and significant tissue regeneration in vivo. [3][4][5] A system that is both simple and reproducible, which can be used either in the laboratory to pass to the surgical room or directly during surgery, is also important.…”

Section: Introductionmentioning

confidence: 99%

“…Hydrogel biopolymers such as collagen, alginate, and chitosan have shown cellular compatibility and the capacity to load and carry cells to the target tissues. [5][6][7][8] Collagen can load cells and has good 1 biocompatibility, while maintaining cellular functions that mimic the native three-dimensional (3D) tissue environment. 9 Collagen also becomes a gel when pH, ionic concentration, and temperature are modulated to near biological conditions.…”

Section: Introductionmentioning

confidence: 99%

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Utilizing Core–Shell Fibrous Collagen-Alginate Hydrogel Cell Delivery System for Bone Tissue Engineering

Pérez

Kim

et al. 2014

Tissue Engineering Part A

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Three-dimensional matrices that encapsulate and deliver stem cells with defect-tuned formulations are promising for bone tissue engineering. In this study, we designed a novel stem cell delivery system composed of collagen and alginate as the core and shell, respectively. Mesenchymal stem cells (MSCs) were loaded into the collagen solution and then deposited directly into a fibrous structure while simultaneously sheathing with alginate using a newly designed core-shell nozzle. Alginate encapsulation was achieved by the crosslinking within an adjusted calcium-containing solution that effectively preserved the continuous fibrous structure of the inner cell-collagen part. The constructed hydrogel carriers showed a continuous fiber with a diameter of *700-1000 mm for the core and 200-500 mm for the shell area, which was largely dependent on the alginate concentration (2%-5%) as well as the injection rate (20-80 mL/h). The water uptake capacity of the core-shell carriers was as high as 98%, which could act as a pore channel to supply nutrients and oxygen to the cells. Degradation of the scaffolds showed a weight loss of *22% at 7 days and *43% at 14 days, suggesting a possible role as a degradable tissue-engineered construct. The MSCs encapsulated within the collagen core showed excellent viability, exhibiting significant cellular proliferation up to 21 days with levels comparable to those observed in the pure collagen gel matrix used as a control. A live/dead cell assay also confirmed similar percentages of live cells within the core-shell carrier compared to those in the pure collagen gel, suggesting the carrier was cell compatible and was effective for maintaining a cell population. Cells allowed to differentiate under osteogenic conditions expressed high levels of bone-related genes, including osteocalcin, bone sialoprotein, and osteopontin. Further, when the core-shell fibrous carriers were implanted in a rat calvarium defect, the bone healing was significantly improved when the MSCs were encapsulated, and even more so after an osteogenic induction of MSCs before implantation. Based on these results, the newly designed core-shell collagen-alginate fibrous carrier is considered promising to enable the encapsulation of tissue cells and their delivery into damaged target tissues, including bone with defect-tunability for bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Utilizing Core–Shell Fibrous Collagen-Alginate Hydrogel Cell Delivery System for Bone Tissue Engineering

Pérez

Kim

et al. 2014

Tissue Engineering Part A

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“…These seasonal changes were observed at least 20 years ago [18] promising [20], it remains to be seen whether this form of packaging will be useful for the AI industry.…”

Section: Sperm Selectionmentioning

confidence: 99%

Animal Reproduction Technologies—Future Perspectives

Morrell¹,

Humblot²

2016

JAST-A

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Abstract:Reproductive biotechnologies, such as artificial insemination, sperm selection and embryo technologies, offer possibilities for animal producers to increase reproductive efficiency. There have been many significant developments in reproductive biotechnologies over the last few decades, e.g., in sperm handling and preservation, in vitro embryo production and preservation, sexing and cloning. This review discusses some of the key changes that have occurred and explores their potential for increasing the reproductive efficiency of domestic animals in the future. As a consequence, they also offer opportunities to facilitate or accelerate genetic selection. If properly used, they may contribute to increase the sustainability of animal production. The role of epigenetics in influencing phenotype is also considered.

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“…As a result of the limitations of 2D cultures, three dimensional cell culturing (3D) in vitro models have already been used in cancer research. The 3D models provide a nearly physiological environment through their complex structure (Ghidoni et al 2008). Cells are able to interact with each other and with extracellular matrix (ECM), which is essential for proliferation, migration and apoptosis (Pampaloni et al 2007;Mountzios et al 2013).…”

Section: Introductionmentioning

confidence: 99%

3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads

et al. 2015

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Cancer diseases are a common problem of the population caused by age and increased harmful environmental influences. Herein, new therapeutic strategies and compound screenings are necessary. The regular 2D cultivation has to be replaced by three dimensional cell culturing (3D) for better simulation of in vivo conditions. The 3D cultivation with alginate matrix is an appropriate method for encapsulate cells to form cancer constructs. The automated manufacturing of alginate beads might be an ultimate method for large-scaled manufacturing constructs similar to cancer tissue. The aim of this study was the integration of full automated systems for the production, cultivation and screening of 3D cell cultures. We compared the automated methods with the regular manual processes. Furthermore, we investigated the influence of antibiotics on these 3D cell culture systems. The alginate beads were formed by automated and manual procedures. The automated steps were processes by the Biomek Ò Cell Workstation (celisca, Rostock, Germany). The proliferation and toxicity were manually and automatically evaluated at day 14 and 35 of cultivation. The results visualized an accumulation and expansion of cell aggregates over the period of incubation. However, the proliferation and toxicity were faintly and partly significantly decreased on day 35 compared to day 14. The comparison of the manual and automated methods displayed similar results. We conclude that the manual production process could be replaced by the automation. Using automation, 3D cell cultures can be produced in industrial scale and improve the drug development and screening to treat serious illnesses like cancer.

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Alginate cell encapsulation: new advances in reproduction and cartilage regenerative medicine

Cited by 94 publications

References 64 publications

Utilizing Core–Shell Fibrous Collagen-Alginate Hydrogel Cell Delivery System for Bone Tissue Engineering

Utilizing Core–Shell Fibrous Collagen-Alginate Hydrogel Cell Delivery System for Bone Tissue Engineering

Animal Reproduction Technologies—Future Perspectives

3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads

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