3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads

Lehmann, R.; Gallert, C.; Roddelkopf, Thomas; Junginger, Steffen; Wree, Andreas; Thurow, Kerstin

doi:10.1007/s10616-015-9861-1

Cited by 9 publications

(4 citation statements)

References 34 publications

(55 reference statements)

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“…The composite skin model was constructed by transferring 400 µl of dermal equivalent collagen solution containing 3x10 5 broblast cells into culture inserts of 24-well format. After polymerization of the gel, 3x10 5 Human epidermal keratinocytes were overlaid on the dermal equivalent and incubate for 2 hr.…”

Section: Generation Of Strati Ed Epidermis In 3d Culturementioning

confidence: 99%

“…However, 2D cultures often lacked the essential components of the natural environment, prompting a shift towards 3D cell culture systems, which are gaining popularity due to their ability to provide a more physiologically relevant environment compared to conventional 2D cultures (3,4). Recent advances in cytotechnology within tissue engineering have been greatly in uenced by the adoption of 3D cell culture systems (5,6).…”

Section: Introductionmentioning

confidence: 99%

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Comparative functional aspects of 3T3 fibroblasts in 2D and 3D cell- culture environment

Chaturvedi,

Chugh,

Raisuddin

et al. 2023

Preprint

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Tissue engineering is a multipurpose and valuable tool extensively employed for tissue architecture, and the evaluation of drug efficacy, with other applications. Its particular focus on techniques aimed at restoring or replacing part or entire tissues of replaceable organs. The development of in-vitro bioengineered skin models holds significant potential for clinical utilization. Most existing skin bioengineering methods primarily rely on two-dimensional (2D) cell cultures, which, however, possess notable limitations, particularly in the context of assessing the safety and effectiveness of topical pharmaceutical agents’ due absence of dermal-epidermal interaction. In contrast, three-dimensional (3D) skin bioengineering model enabling the epidermal keratinocytes culture in combination with fibroblasts embedded within 3D matrices. In this study, we have designed a comprehensive experimental approach to investigate the role of Swiss 3T3 cells and their variants pre-exposed to a single pulse of varying doses of Mitomycin C collagen-embedded in supporting the growth of keratinocytes. We examined the proliferation and viability of 3T3 cells to assess their ability to provide growth of epidermal cells in an embedded niche. Additionally, we analysed the levels of Keratinocyte Growth Factor, paracrine secretion by fibroblasts in both 2D and 3D cell culture condition. Furthermore, we conducted a histological comparison of the epidermal layers constructed under 2D and 3D cell culture conditions, evaluating the expression of various epidermal markers to gain insights into their structural and functional differences. This study sheds light on the pivotal role of 2D and 3D skin bioengineering models and their potential applications in dermatological research and pharmaceutical development.

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Section: Generation Of Strati Ed Epidermis In 3d Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative functional aspects of 3T3 fibroblasts in 2D and 3D cell- culture environment

Chaturvedi,

Chugh,

Raisuddin

et al. 2023

Preprint

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“… 5 – 8 Robotic arm-assisted systems that utilize articulated robots for autonomous operation are another example. 9 – 15 One drawback of specialized systems is that the addition or modification of experimental instruments and procedures poses certain challenges. Because of this lack of flexibility, specialized automated culture systems are generally ill-suited for basic research as the addition of new experiments, the modification of protocols, and the addition of new laboratory equipment are frequently required.…”

Section: Introductionmentioning

confidence: 99%

A Variable Scheduling Maintenance Culture Platform for Mammalian Cells

et al. 2021

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Cell culturing is a basic experimental technique in cell biology and medical science. However, culturing high-quality cells with a high degree of reproducibility relies heavily on expert skills and tacit knowledge, and it is not straightforward to scale the production process due to the education bottleneck. Although many automated culture systems have been developed and a few have succeeded in mass production environments, very few robots are permissive of frequent protocol changes, which are often required in basic research environments. LabDroid is a general-purpose humanoid robot with two arms that performs experiments using the same tools as humans. Combining our newly developed AI software with LabDroid, we developed a variable scheduling system that continuously produces subcultures of cell lines without human intervention. The system periodically observes the cells on plates with a microscope, predicts the cell growth curve by processing cell images, and decides the best times for passage. We have succeeded in developing a system that maintains the cultures of two HEK293A cell plates with no human intervention for 192 h.

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“…For complete details on the use and execution of this protocol, please refer to Lhuissier et al. (2017) , Lehmann et al. (2016) , Liu et al.…”

mentioning

confidence: 99%

Alginate-based 3D cancer cell culture for therapeutic response modeling

et al. 2021

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Summary Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients. Here, we describe a protocol for a facile three-dimensional (3D) culture format for cancer cells. This 3D platform helps overcome the 2D culture limitations. In addition, it allows for longitudinal modeling of responses to cancer therapeutics. For complete details on the use and execution of this protocol, please refer to Lhuissier et al. (2017) , Lehmann et al. (2016) , Liu et al. (2016) , and Duval et al. (2011) .

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3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads

Cited by 9 publications

References 34 publications

Comparative functional aspects of 3T3 fibroblasts in 2D and 3D cell- culture environment

Comparative functional aspects of 3T3 fibroblasts in 2D and 3D cell- culture environment

A Variable Scheduling Maintenance Culture Platform for Mammalian Cells

Alginate-based 3D cancer cell culture for therapeutic response modeling

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