Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications

Lou, Ruyun; Xie, Hehu; Zheng, Huizhen; Ren, Ying; Gao, Meng; Guo, Xin; Song, Yukun; Wu, Yu; Liu, Xiudong; Ma, Xiaojun

doi:10.1016/j.ijbiomac.2016.09.078

Cited by 24 publications

(13 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover the calcium deposition was increased in the presence of nHA. These results are in compliance with other studies, which used these microcapsules on different stem cells (45,48,73). However, this study evaluated these modular microcapsules in osteogenic differentiation of hDPSCs for the rst time, based on authors' information.…”

Section: Discussionsupporting

confidence: 85%

The Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Dental Pulp in Modular Alginate-Gelatin/Nano-Hydroxyapatite Microcapsules

Alipour

Firouzi

Aghazadeh

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Microcapsule is considered as a promising 3D microenvironment for Bone Tissue Engineering (BTE) applications. Microencapsulation of cells in an appropriate scaffold not only protects cells against excess stress but also promotes cell proliferation and differentiation. Methods In the current study, human Dental Pulp Stem Cells (hDPSCs) were cultivated in Alginate/Gelatin (Alg/Gel) and Alginate/Gelatin/nano-Hydroxyapatite (Alg/Gel/nHA) microcapsules. The proliferation and osteogenic differentiation of these cells were evaluated by MTT assay, qRT-PCR, Alkaline phosphatase, and Alizarine Red S.Results The results revealed that microencapsulation by Alg/Gel/nHA could improve cell proliferation and induce osteogenic differentiation. The cells cultured in the Alg/Gel and Alg/Gel/nHA microcapsules after 28 days showed 2.5-fold and 4-fold more activity of BMP-2 gene expression in comparison with the cells cultured on the polystyrene surface as a control group. The nHA addition to hDPSCs-laden Alg/Gel microcapsule could also up-regulate the bone-related gene expressions of osteocalcin, osteonectin, and RUNX-2 during a 28-day culture period. Calcium deposition and ALP activities of the cells were obsereved in accordance with the proliferation results as well as the gene expression analysis. Conclusion The study demonstrated that microencapsulation of hDPSCs inside Alg/Gel/nHA hydrogel can be a potential approach for regenerative dentistry in the near future.

show abstract

Section: Discussionsupporting

confidence: 85%

The Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Dental Pulp in Modular Alginate-Gelatin/Nano-Hydroxyapatite Microcapsules

Alipour

Firouzi

Aghazadeh

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The most crucial advantage of hepatocyte encapsulation with alginate is biocompatibility and a high rate of cell viability, which is extremely important for cell functions. 36,174 Besides, this material shows high permeability due to its range of pores in the porous structure.…”

Section: Extracellular Matrixmentioning

confidence: 99%

“…HepG2 cell lines were encapsulated in alginate and chitosan polymer, and 78% of HepG2 cells were viable. 174 MSC, mesenchymal stem cell; PLL, poly-l-lysine.…”

Section: Extracellular Matrixmentioning

confidence: 99%

“…This demonstrates that chitosan can be used as an additive or a shell and allows encapsulated cells to have better functions when compared with alginate, but it is not promising as a core material for encapsulation. 174 Encapsulation of HepG2 cell lines shows extremely high secretion despite being a cell line (600 ng/24 h/10 6 cells) in silk-sericin. Other materials like PEG did not have significant secretion compared with alginate and silk-sericin.…”

Section: Liver Tissue Engineering 155mentioning

confidence: 99%

“…It shows that alginate itself is not the best environment for the secretion of this metabolite, but in other studies, the use of additives like chitosan and galactosylated chitosan showed a better synthesis of this metabolite (150 mg/ 10 6 cells). 174 As mentioned before, alginate is not a suitable substrate for the secretion of urea, although to overcome this challenge, adding other materials, such as chitosan, for enhancing the adherence can be useful. Encapsulating iPSCs that are differentiated to endothelial cells and hepatocytes is another way to increase the level of synthesis of this metabolite.…”

Section: Liver Tissue Engineering 155mentioning

confidence: 99%

See 2 more Smart Citations

Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment

Mirdamadi

Kalhori

Zakeri

et al. 2020

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

Chronic liver diseases affect thousands of lives throughout the world every year. The shortage of liver donors for transplantation has been the main driving force to employ alternative methods such as liver tissue engineering (LTE) in fabricating a three-dimensional transplantable liver tissue or enhancing cell delivery techniques alleviating the need for liver donors. LTE consists of three components, cells, ECM (extracellular matrix), and signaling molecules, which we discuss the first and second. The three most common cell sources used in LTE are human and animal primary hepatocytes, and stem cells for different applications. Two major categories of ECM are used to mimic the microenvironment of these cells, named scaffolds and microbeads. Scaffolds have been made by numerous methods with a wide range of synthetic and natural biomaterials. Cell encapsulation has also been utilized by many polymeric biomaterials. To investigate their functions, many properties have been discussed in the literature, such as biochemical, geometrical, and mechanical properties, in both of these categories. Overall, LTE shows excellent potential in assisting hepatic disorders. However, some challenges exist that prevent the practical use of it clinically, making LTE an ongoing research subject in the scientific society.

show abstract

Alginate‐Based Bio‐Nanohybrids with Unique Properties for Biomedical Applications

et al. 2022

View full text Add to dashboard Cite

Alginate is among the widely applied polysaccharide‐based biomaterial owing to its natural occurrence, chemical versatility, non‐toxicity, environmental friendliness, unique biocompatibility, biodegradability, and mucoadhesive attributes. Nevertheless, it is associated with some demerits, such as inadequate mechanical features and biological activity that hamper broad‐spectrum biomedical scope. Different biopolymeric and nanomaterials have been incorporated into the alginate framework to develop robust nanoplatforms with desired functionalities to overcome this inadequacy. Hybrids of alginate with biopolymers and nanostructured materials improve its mechanical and physicochemical characteristics and may also boost its biological activity. This review aimed to summarize the ongoing progress in alginate‐based nanoplatforms for potential applications in biomedical sectors. Combining alginate with biopolymers (chitosan, starch) and nanostructured materials (silica nanoparticles, graphene oxide nanoparticles, carbon nanotubes/nanorods, and metal oxide nanoparticles) are particularly discussed for drug delivery, wound dressing, tissue engineering, and 3D bioprinting applications. The ongoing challenging facets and futuristic directions on alginate blends are also spotlighted. The alginate‐based hybrids will likely demonstrate biocompatibility, enhanced cell adhesion, cell proliferation, desired mechanical strength, and excellent mineralization. Therefore, they are considered bio‐inspired materials for futuristic biomedical applications.

show abstract

Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications

Cited by 24 publications

References 42 publications

The Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Dental Pulp in Modular Alginate-Gelatin/Nano-Hydroxyapatite Microcapsules

The Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Dental Pulp in Modular Alginate-Gelatin/Nano-Hydroxyapatite Microcapsules

Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment

Alginate‐Based Bio‐Nanohybrids with Unique Properties for Biomedical Applications

Contact Info

Product

Resources

About