Alginate based hydrogel inks for 3D bioprinting of engineered orthopedic tissues

Murab, Sumit; Gupta, Aastha; Włodarczyk‐Biegun, Małgorzata K.; Kumar, Anuj; Rijn, Patrick van; Whitlock, Patrick W.; Han, Sung Soo; Agrawal, Garima

doi:10.1016/j.carbpol.2022.119964

Cited by 53 publications

(29 citation statements)

References 107 publications

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“…Scaffolds applied for bone TE and tissue regeneration should have good biocompatibility, osteoconductivity, and biodegradability/bioactivity, along with the promotion of osseointegration as well as stimulation of ingrowth and differentiation of bone [ 70 , 71 , 72 ]. Different alginate-based composites have been introduced for bone TE applications, including alginate-polymers (e.g., chitosan or PEG), alginate-biosilica, alginate-ceramics, alginate-bioglasses, alginate-bone morphogenetic protein-2, and alginate-proteins (e.g., collagen or gelatin).…”

Section: Te-rm Applicationsmentioning

confidence: 99%

Alginate-Based Biomaterials in Tissue Engineering and Regenerative Medicine

2023

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Today, with the salient advancements of modern and smart technologies related to tissue engineering and regenerative medicine (TE-RM), the use of sustainable and biodegradable materials with biocompatibility and cost-effective advantages have been investigated more than before. Alginate as a naturally occurring anionic polymer can be obtained from brown seaweed to develop a wide variety of composites for TE, drug delivery, wound healing, and cancer therapy. This sustainable and renewable biomaterial displays several fascinating properties such as high biocompatibility, low toxicity, cost-effectiveness, and mild gelation by inserting divalent cations (e.g., Ca2+). In this context, challenges still exist in relation to the low solubility and high viscosity of high-molecular weight alginate, high density of intra- and inter-molecular hydrogen bonding, polyelectrolyte nature of the aqueous solution, and a lack of suitable organic solvents. Herein, TE-RM applications of alginate-based materials are deliberated, focusing on current trends, important challenges, and future prospects.

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Section: Te-rm Applicationsmentioning

confidence: 99%

Alginate-Based Biomaterials in Tissue Engineering and Regenerative Medicine

2023

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“…A flexible transparent electrode with excellent photoelectric performance, mechanical stability, and environmental adaptability are prepared on the adjustable transparency base. The transparent electrode with transparent light transparency is excellent ( Murab et al, 2022 ). Although these standard 3D printing processes can achieve higher-precision pattern electrodes and microstructure printing, this type of conductive material based on the point-to-line processing method is not only deficient in efficiency.…”

Section: Polymer Materials For 3d Bio Printing Of Bone and Cartilagementioning

confidence: 99%

“…The processing accuracy is related to the diameter of the nozzle of the extrusion material, so the higher the printing accuracy, the slower the printing speed. In contrast, digital light treatment (DLP) printing shows the advantages of high printing accuracy, fast relative velocity, and superior surface quality, which provides high-performance conductive material and devices for manufacturing negative perpopy pine ratio, complicated geometric shapes, and micro-surface structures ( Murab et al, 2022 ).…”

Section: Polymer Materials For 3d Bio Printing Of Bone and Cartilagementioning

confidence: 99%

3D printing of bone and cartilage with polymer materials

Fan

Liu²,

Wang³

et al. 2022

Front. Pharmacol.

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Damage and degeneration to bone and articular cartilage are the leading causes of musculoskeletal disability. Commonly used clinical and surgical methods include autologous/allogeneic bone and cartilage transplantation, vascularized bone transplantation, autologous chondrocyte implantation, mosaicplasty, and joint replacement. 3D bio printing technology to construct implants by layer-by-layer printing of biological materials, living cells, and other biologically active substances in vitro, which is expected to replace the repair mentioned above methods. Researchers use cells and biomedical materials as discrete materials. 3D bio printing has largely solved the problem of insufficient organ donors with the ability to prepare different organs and tissue structures. This paper mainly discusses the application of polymer materials, bio printing cell selection, and its application in bone and cartilage repair.

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“…Alginate shows good printability by rapid gelling in presence of calcium ion which makes it attractive biomaterial ink for 3D printing. 33 Chitosan (CH) is a cationic natural biopolymer obtained from partially deacetylation of chitin and it contains amino (ÀNH 2 ) /acetamide (e.g. ÀNHCOCH 3 ) groups, and primary and secondary hydroxyl groups.…”

Section: Introductionmentioning

confidence: 99%

“…In 3D printing, the printability of biomaterial is a basic challenge. Alginate shows good printability by rapid gelling in presence of calcium ion which makes it attractive biomaterial ink for 3D printing 33 …”

Section: Introductionmentioning

confidence: 99%

Fabrication and investigation of physicochemical and biological properties of3Dprinted sodium alginate‐chitosan blend polyelectrolyte complex scaffold for bone tissue engineering application

Singh

Pramanik

2023

J of Applied Polymer Sci

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In tissue engineering technique, a biological scaffold with appropriate composition and structure for promoting growth and differentiation of cells thereby regenerating damaged tissue, is a prime necessity. In this paper, 3D‐printed scaffolds comprising sodium alginate (SA) and chitosan (CH) biopolymers of natural origin are reported. Bioinks with varying ratios of SA and CH were prepared and scaffolds were fabricated by 3D printing. The fabricated scaffolds possess many desired properties that are vital for tissue regeneration purposes. The scaffolds possess open pore microstructures with interconnected pores and desired pore size as revealed by scanning electron microscopic image analysis. The polyelectrolyte complex formation (PEC) between SA and CH as revealed by Fourier‐transform‐infrared spectroscopic analysis is favorable as it offers a better surface for cell attachment and proliferation, and an ideal microenvironment for bone regeneration. Among the scaffolds, SA/CH with 60:40 showed controlled swelling and degradation behavior, with higher tensile strength of 0.387 ± 0.015 MPa. In vitro‐biomineralization showed superior apatite layer deposition ability over the SA/CH: 60/40 scaffold surface. The fabricated SA/CH scaffolds are hydrophilic and biocompatible as evident from the contact angle, protein adsorption, MTT assay, and cell attachment studies. However, SA/CH: 60/40 is shown to have superior biological properties compared with the other SA/CH compositions. Thus, it is concluded that 3Dv printed SA/CH: 60/40 scaffold having some superior properties and bioactivity can be used as a suitable matrix for future bone tissue regeneration applications.

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Alginate based hydrogel inks for 3D bioprinting of engineered orthopedic tissues

Cited by 53 publications

References 107 publications

Alginate-Based Biomaterials in Tissue Engineering and Regenerative Medicine

Alginate-Based Biomaterials in Tissue Engineering and Regenerative Medicine

3D printing of bone and cartilage with polymer materials

Fabrication and investigation of physicochemical and biological properties of3Dprinted sodium alginate‐chitosan blend polyelectrolyte complex scaffold for bone tissue engineering application

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