Alginate-Based Hydrogel Containing Taurine-Loaded Chitosan Nanoparticles in Biomedical Application

Abstract

The hydrogel efficacy of taurine-loaded chitosan nanoparticle/alginate hydrogel was investigated for controlled release of the taurine substrate, which is known as an antioxidative drug. The composition of the fabricated hydrogels was explored by Fouriertransform infrared spectroscopy. The swelling ability and degradation rate of hydrogels were also analyzed in phosphate-buffered saline at the physiological condition and alginate lyase for a period of 21 days. Moreover, morphologies and structure of hydrogels and cells were determined using a scanning electron microscope. The possible cytotoxicity of the fabricated hydrogel was carried out by seeding endometrial stem cells on hydrogels. The results demonstrated that hydrogel of chitosan nanoparticle/alginate hydrogel successfully controlled the release of taurine. We observed that the chitosan nanoparticle/alginate hydrogel possessed adjust swelling ability and degradation rate as compared to neat alginate hydrogel. The results proved that the chitosan nanoparticle/alginate hydrogel is non-cytotoxic and could be utilized as a promising composition in tissue engineering and drug delivery systems.

Layered dermal reconstitution through epigallocatechin 3-gallate loaded chitosan nanoparticle within enzymatically crosslinked polyvinyl alcohol/collagen fibrous mat

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Hyaluronic acid/gelatin microcapsule functionalized with carbon nanotube through laccase‐catalyzed crosslinking for fabrication of cardiac microtissue

“…Water contact angle analysis was utilized to assess hydrogel's hydrophilic/hydrophobic properties, which regulate protein penetration and cellular adhesion 37–40 . In fact, for cell attachment, in addition to the possible availability of cell‐adhesive ligands, the cells tend to attach to surfaces with water contact angels in the interval of 60°–80° 22,28,40–42 .…”

Impact of exosome‐loaded chitosan hydrogel in wound repair and layered dermal reconstitution in mice animal model

“…The synergistic effect of EnSCs on tissue regeneration of various tissues such as nerve, bone, cartilage, and skin proved its potential applicability in tissue engineering and regenerative medicine (Bahrami et al, 2018;Ebrahimi-Barough et al, 2013;Goodarzi et al, 2019;Zahiri et al, 2019). The hEnSC has ability to secret large amount of bioactive molecules including cytokines, growth factors, as well as other paracrine factors which can be used as a promising approach to design efficient bioactive scaffolds for tissue engineering or tissue repair (Cooper et al, 2018;Lai et al, 2010;Roşca, Ţuţuianu, & Titorencu, 2018;Shabbir et al, 2015;Shafei et al, 2020).…”