Alginate-Based Cryogels for Combined Chemo/Photothermal Antibacterial Therapy and Rapid Hemostasis

Abstract

As novel wound dressings, cryogels with rapid hemostatic property and good sterilization effect are urgently desirable for wound healing. To reduce the use of antibiotics, antibacterial photothermal therapy with broad-spectrum bactericidal capacity and non-obvious bacterial resistance has been widely researched. However, photothermal agents usually suffer from poor hemostatic ability. In this research, sodium alginate (SA) and epigallocatechin gallate (EGCG) were non-covalently cross-linked in suit by ferric ions to obtain SA/EGCG/Fe (SEF) cryogels after lyophilization as an antibacterial wound dressing. Next, its photothermal performance was intensively assessed. Moreover, its hemostasis and bactericidal effect were evaluated. First, it displayed extraordinary photothermal ability owing to the formation of Fe3+/EGCG-based metal phenolic networks (MPNs) inside the SEF cryogel. Furthermore, in vitro and in vivo assays illustrated that it exhibits rapid hemostatic capacity owing to its high porosity and MPN-mediated cell adhesion capacity. In conclusion, the SEF cryogel manifests satisfactory hemostatic and bactericidal properties. Therefore, it is a promising wound-dressing candidate for clinical applications.

A Cysteine-Maleimide-Based Design for Hemostatic, Antibacterial, and Biodegradable Wound Dressing

“…Afterward, centrifugation at 116 g for 10 min was performed, followed by the collection of 200 μL of the resulting supernatant. The absorbance at 540 nm was measured by utilizing a spectrophotometer, with a higher absorbance value indicating decreased blood clotting function …”

“…The absorbance at 540 nm was measured by utilizing a spectrophotometer, with a higher absorbance value indicating decreased blood clotting function. 57 Antibacterial Activity Test in Vitro. We determined the antibacterial activity against Gram-negative E. coli (ATCC 25922) and Gram-positive S.aureus (ATCC 25923, generously supplied by Professor Weihui Wu from the College of Life Sciences, Nankai University) by the Kirby Bauer disk diffusion method.…”

Recent Advances in Topical Hemostatic Materials

“…SA/EGCG/Fe (SEF) possessed good hemostatic properties, which were attributed to the high porosity and MPN-mediated cell adhesion ability as well as excellent photothermal ability for photothermal antibacterial effects. 85…”

“…Lin et al used sodium alginate (SA) and epigallocatechin gallate (EGCG) by noncovalent cross-linking via ferric ions to obtain SA/EGCG/Fe (SEF). SA/EGCG/Fe (SEF) possessed good hemostatic properties, which were attributed to the high porosity and MPN-mediated cell adhesion ability as well as excellent photothermal ability for photothermal antibacterial effects …”

Dynamic Microenvironment-Adaptable Hydrogel with Photothermal Performance and ROS Scavenging for Management of Diabetic Ulcer

“…Photothermal therapy (PTT) is a nonantibiotic strategy that converts light energy into heat energy driven by an external light source to eliminate pathogens at the wound site. − PTT has been recognized as an alternative treatment for infectious diseases owing to its unique advantages, including improved selectivity, minimal invasiveness, and side effects . Owing to the high photothermal conversion ability, unique local surface plasmonic resonance (LSPR) effect, and good biocompatibility, Au nanorods (AuNRs) have been widely used in PTT .…”