Alginate as a Cell Culture Substrate for Growth and Differentiation of Human Retinal Pigment Epithelial Cells

Heidari, Razeih; Soheili, Zahra‐Soheila; Samiei, Shahram; Ahmadieh, Hamid; Davari, Maliheh; Nazemroaya, Fatemeh; Bagheri, Abouzar; Deezagi, Abdolkhalegh

doi:10.1007/s12010-014-1431-z

Cited by 27 publications

(13 citation statements)

References 30 publications

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“…In a study, a thin film of purified alginate was used to demonstrate its ability to support the growth of RPE cells and their high proliferative rates [84]. In another study, alginate beads were used to demonstrate RPE cell sustenance and proliferation [85]. An arginine-glycine-aspartic-alginate (RGD-alginate) scaffold demonstrated feasibility for cell derivation and transplantation of RPE and neural retina [86].…”

Section: Biomaterials and Scaffolds Used For Tissue Engineering 41 Natural Biomaterials Used As Scaffoldsmentioning

confidence: 99%

Tissue Engineering Strategies for Retina Regeneration

et al. 2021

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The retina is a complex and fragile photosensitive part of the central nervous system which is prone to degenerative diseases leading to permanent vision loss. No proven treatment strategies exist to treat or reverse the degenerative conditions. Recent investigations demonstrate that cell transplantation therapies to replace the dysfunctional retinal pigment epithelial (RPE) cells and or the degenerating photoreceptors (PRs) are viable options to restore vision. Pluripotent stem cells, retinal progenitor cells, and somatic stem cells are the main cell sources used for cell transplantation therapies. The success of retinal transplantation based on cell suspension injection is hindered by limited cell survival and lack of cellular integration. Recent advances in material science helped to develop strategies to grow cells as intact monolayers or as sheets on biomaterial scaffolds for transplantation into the eyes. Such implants are found to be more promising than the bolus injection approach. Tissue engineering techniques are specifically designed to construct biodegradable or non-degradable polymer scaffolds to grow cells as a monolayer and construct implantable grafts. The engineered cell construct along with the extracellular matrix formed, can hold the cells in place to enable easy survival, better integration, and improved visual function. This article reviews the advances in the use of scaffolds for transplantation studies in animal models and their application in current clinical trials.

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Section: Biomaterials and Scaffolds Used For Tissue Engineering 41 Natural Biomaterials Used As Scaffoldsmentioning

confidence: 99%

Tissue Engineering Strategies for Retina Regeneration

et al. 2021

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show abstract

“…In a study, thin film of purified alginate was used to demonstrate its ability to support the growth of RPE cells and their high proliferative rates [84]. In another study, alginate beads were used to demonstrate RPE cell sustenance and proliferation [85]. An arginine-glycine-aspartic-alginate (RGD-alginate) scaffold demonstrated feasibility for cell derivation and transplantation of RPE and neural retina [86].…”

Section: Natural Biomaterials Used As Scaffoldsmentioning

confidence: 99%

Tissue Engineering Strategies for Retinal Regeneration

Ds¹,

Seiler²,

Patel³

et al. 2021

Preprint

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The retina is a complex and fragile photosensitive part of the central nervous system which is prone to degenerative diseases leading to permanent vision loss. No proven treatment strategies exist to treat or reverse the degenerative conditions. Recent investigations demonstrate that cell transplantation therapies to replace the dysfunctional retinal pigment epithelial (RPE) cells and or the degenerating photoreceptors (PRs) are viable options to restore vision. Pluripotent stem cells, retinal progenitor cells and somatic stem cells are the main cell sources used for cell transplantation therapies. The success of retinal transplantation based on cell suspension injection is hindered by limited cell survival and lack of cellular integration. Recent advances in material science helped to develop strategies to grow cells as intact monolayers or as sheets on biomaterial scaffolds for transplantation into the eyes. Such implants are found to be more promising than the bolus injection approach. Tissue engineering techniques are specifically designed to construct biodegradable or non-degradable polymer scaffolds to grow cells as a monolayer and construct implantable grafts. The engineered cell construct along with the extracellular matrix formed, can hold the cells in place to enable easy survival, better integration and improved visual function. This article reviews the advances in the use of scaffolds for transplantation studies in animal models and its application in current clinical trials.

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“…Heidari et al evaluated the behavior of human RPE cells on alginate beads as a cell culture substrate and showed that RPE cells could grow and proliferate on alginate beads in vitro. However, consecutive passages of cells caused the cells to lose their pigments [73]. Hunt et al further demonstrated the potential of RGD-alginate scaffolds for the derivation, transport, and transplantation of neural retina and RPE [44].…”

Section: Scaffold Biocompatibilitymentioning

confidence: 99%

Retinal cell regeneration using tissue engineered polymeric scaffolds

Zadeh

Khoder

Al-Kinani

et al. 2019

Drug Discovery Today

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Highlights  There is no approved treatment for dry AMD or for the advanced GA of the retina.  Tissue engineering is promising to repair damaged human retina, and restore its functions.  Polymeric scaffolds allow cells to grow & proliferate to regenerate damaged retinal tissues.  Integration of tissue engineering with drug delivery to regenerate retinal cells is yet to be realized. Degenerative retinal diseases, such as age-related macular degeneration (AMD), can lead to permanent sight loss. Although intravitreal anti-vascular endothelial growth factor (VEGF) and steroid injections are effective for the management of early stages of wet and/or neovascular AMD (nAMD), no proven treatments currently exist for dry AMD or for the advanced geographic atrophy of the retina that follows. Tissue engineering (TE) has recently emerged as a promising alternative to repair retinal damaged and restore its functions. Here, we review recent advances in TE, with a particular emphasis on retinal regeneration. We provide an overview of retinal diseases, followed by a comprehensive review of TE techniques, cells, and polymers used in the fabrication of scaffolds for retinal cell regenerations, in particular the retinal pigment epithelium (RPE).

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Alginate as a Cell Culture Substrate for Growth and Differentiation of Human Retinal Pigment Epithelial Cells

Cited by 27 publications

References 30 publications

Tissue Engineering Strategies for Retina Regeneration

Tissue Engineering Strategies for Retina Regeneration

Tissue Engineering Strategies for Retinal Regeneration

Retinal cell regeneration using tissue engineered polymeric scaffolds

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