Alginate- and gelatin-based bioactive photocross-linkable hybrid materials for bone tissue engineering

Abstract

The paper presents the synthesis, the physico-chemical and the biological properties of novel hybrid materials prepared from photo-crosslinked gelatin/alginate-based hydrogels and silica particles exhibiting potential for the regeneration of bone tissue. Both alginate and gelatin were functionalized with methacrylate and methacrylamide moieties, respectively to render them photo-crosslinkable. Submicron silica particles of two sizes were dispersed within three types of polymeric sols including alginate, gelatin, and gelatin/alginate blends, which were subsequently photo-crosslinked. The swelling ratio, the gel fraction and the mechanical properties of the hybrid materials developed were examined and compared to these determined for reference hydrogel matrices. The in vitro cell culture studies have shown that the prepared materials exhibited biocompatibility as they supported both MEFs and MG-63 mitochondrial activity. Finally, the in vitro experiments performed under simulated body fluid conditions have revealed that due to inclusion of silica particles into the biopolymeric hydrogel matrices the mineralization was successfully induced.

Development prospects of curable osteoplastic materials in dentistry and maxillofacial surgery

“…Hydrogen bonds between the components form a hydrogel based on alginate and gelatin with the addition of silica particles. In the study by Lewandowska-Lancucka et al (2017), the material was cured by UV radiation [ 86 ]. The gelation reaction lasted for 90 min.…”

Polysaccharide-Based Systems for Targeted Stem Cell Differentiation and Bone Regeneration

“…SiO 2 -nanoparticles are incorporated in a hydrogel fabricated by photocrosslinking of methacrylateand methacrylamide-functionalized alginate and gelatin. Prepared hybrid materials exhibited cytocompatibility with MG-63 osteoblast like cells as well as mouse embryonic fibroblasts [143]. In a following study, the authors used genipin-cross-linked chitosan and collagen as matrix material for the incorporation of SiO 2 -NPs of 240 or 450 nm in diameter.…”

An approach for mechanical property optimization of cell-laden alginate–gelatin composite bioink with bioactive glass nanoparticles

“…However, alginate lacks cell adhesion motifs, which is a crucial feature toward cell adhesion and proliferation [7]. For this reason, alginate has always been combined with proteins such as gelatin that contain cell adhesion motifs in order to create a novel hydrogel composite [8][9][10]. Gelatin is a biodegradable protein derived from natural polymer collagen protein, which has unique features including excellent biocompatibilities and nonimmunogenicities [11].…”