Alginate and chitosan polyion complex hybrid fibers for scaffolds in ligament and tendon tissue engineering

Majima, Tokifumi; Funakosi, Tadanao; Iwasaki, Norimasa; Yamane, Shintarou; Harada, Kaoru; Nonaka, Sachiko; Minami, Akio; Nishimura, Shin‐Ichiro

doi:10.1007/s00776-005-0891-y

Cited by 185 publications

(87 citation statements)

References 24 publications

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“…Some approaches involve materials currently used as suture material, including processed silk fibers [1,12] and bioabsorbable polymers [7,34]. Biologic substrates, including collagen gels [2,6,27], hyaluronan [17], and alginate/chitosan polymers [36] have also been used.…”

Section: Discussionmentioning

confidence: 99%

The Effects of GDF-5 and Uniaxial Strain on Mesenchymal Stem Cells in 3-D Culture

Farng

Urdaneta²,

Barba³

et al. 2008

Clin Orthop Relat Res

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Recent endeavors in tissue engineering have attempted to identify the optimal parameters to create an artificial ligament. Both mechanical and biochemical stimulation have been used by others to independently modulate growth and differentiation, although few studies have explored their interactions. We applied previously described fabrication techniques to create a highly porous (90%-95% porosity, 212-300 lm), 3-D, bioabsorbable polymer scaffold (polycaprolactone). Scaffolds were coated with bovine collagen, and growth and differentiation factor 5 (GDF-5) was added to half of the scaffolds. Scaffolds were seeded with mesenchymal stem cells and cultured in a custom bioreactor under static or cyclic strain (10% strain, 0.33 Hz) conditions. After 48 hours, both mechanical stimulation and GDF-5 increased mRNA production of collagen I, II, and scleraxis compared to control; tenascin C production was not increased. Combining stimuli did not change gene expression; however, cellular metabolism was 1.7 times higher in scaffolds treated with both stimuli. We successfully grew a line of mesenchymal stem cells in 3-D culture, and our initial data indicate mechanical stimulation and GDF-5 influenced cellular activity and mRNA production; we did not, however, observe additive synergism with the mechanical and biological stimuli.

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Section: Discussionmentioning

confidence: 99%

The Effects of GDF-5 and Uniaxial Strain on Mesenchymal Stem Cells in 3-D Culture

Farng

Urdaneta²,

Barba³

et al. 2008

Clin Orthop Relat Res

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show abstract

“…However, complete replacement based on silk did not yield a functional therapy [382], which, in addition to the concerns of allergic reaction, have restricted its use in regenerative medicine [373]. To a smaller extent, chitosan / alginate composites have been assessed for tendon repair, with in vitro data demonstrating adherence of rabbit tendon fibroblast and increased collagen type I expression and mechanical properties [383]. Preclinical data of chitosan and chitosan / HA scaffolds have demonstrated enhanced tissue-specific ECM production and improved mechanical strength, as compared to the control treatments [384][385][386][387].…”

Section: Bottom-up Approached For Tendon Repair Based On Natural In Omentioning

confidence: 99%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

172

188

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“…The resultant fibers were found to have high absorbency as well as sustained antimicrobial properties Miraftab et al [11]. Majima et al [12] found that the poly blend fibers made from alginate and chitosan have better biocompatibility and cell affinity, which are suitable for the preparation of scaffolds in tissue engineering.…”

Section: Fiber Productionmentioning

confidence: 99%

Marine Bioactive Fibers: Alginate and Chitosan Fibers-A Critical Review

Qin¹,

Deng²,

Hao³

et al. 2017

JTEFT

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Alginate and chitosan are two marine natural polymers with unique structures and properties. Alginate is a polymeric acid, whilst chitosan is a polymeric amine. Both polymers can be made into fibers through the wet-spinning process. This article reviews the novel properties of alginate and chitosan, and compares the functional performances of the two fibers as novel textile materials. The principal applications of alginate and chitosan fibers are also reviewed.

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Alginate and chitosan polyion complex hybrid fibers for scaffolds in ligament and tendon tissue engineering

Cited by 185 publications

References 24 publications

The Effects of GDF-5 and Uniaxial Strain on Mesenchymal Stem Cells in 3-D Culture

The Effects of GDF-5 and Uniaxial Strain on Mesenchymal Stem Cells in 3-D Culture

The past, present and future in scaffold-based tendon treatments

Marine Bioactive Fibers: Alginate and Chitosan Fibers-A Critical Review

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