Algibacter aquimarinus sp. nov., isolated from a marine environment, and reclassification of Pontirhabdus pectinivorans as Algibacter pectinivorans comb. nov.

Abstract

Algibacter aquimarinus sp. nov., isolated from a marine environment, and reclassification of Pontirhabdus pectinivorans as Algibacter pectinivorans comb. nov. T , was isolated from seawater collected from Gwangyang Bay, Republic of Korea. The isolate required sea salts for growth. Gliding motility was observed. Flexirubin-type pigments were absent. Major cellular fatty acids (.10 % of the total) were iso-C 15 : 0 , iso-C 17 : 0 3-OH, iso-C 15 : 1 G, C 16 : 0 , iso-C 15 : 0 3-OH and C 18 : 0 . The main polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The predominant isoprenoid quinone was MK-6. The DNA G+C content was 38.6±0.7 mol% (mean±SD of three determinations). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KYW589T belongs to the family Flavobacteriaceae, and was related to the genus Algibacter. Based on data from a study using a polyphasic taxonomic approach, it is proposed that strain KYW589 T represents a novel species belonging to the genus Algibacter, for which the name Algibacter aquimarinus sp. nov. is proposed. (2011) and encompasses only one species with a validly published name, Pontirhabdus pectinivorans, which is nonagarolytic. During a study of the microbial diversity of the seawater of Gwangyang Bay, an orange-pigmented flavobacterial strain, designated KYW589 T , was isolated and was studied according to the minimal standards for the description of new taxa of the family Flavobacteriaceae (Bernardet, 2010;Bernardet et al., 2002). Further, the intermediate phylogenetic position of the new isolate between members of the genera Algibacter and Pontirhabdus and the chemotaxonomic similarity of the two genera necessitated a re-evaluation of the taxonomic status of the genera Algibacter and Pontirhabdus. Strain KYW589T was isolated from a seawater sample obtained at 34 u 539 N 127 u 469 E by using a standard dilution-plating method on marine agar 2216 (MA; Becton Dickinson). The isolate was routinely cultured on MA and maintained as glycerol suspensions (20 %, w/v) at 280 u C. P. pectinivorans KACC 14153 T , A. lectus KCTC 12103 T and A. mikhailovii KCTC 12710T were selected as reference strains and evaluated together under experimental conditions identical to those used for strain KYW589T .Bacterial DNA preparation and PCR amplification and sequencing of the 16S rRNA gene were carried out as described previously (Chun & Goodfellow, 1995). Identification of phylogenetic neighbours and calculation of pairwise 16S rRNA gene sequence similarities were 3Present address:

Algibacter undariae sp. nov., isolated from a brown algae reservoir

“…The fatty acid profiles of the three strains grown and analysed under identical conditions in this study were similar, but there were also differences in the proportions of some fatty acids. A. mikhailovii LMG 23988 T was characterized by having a higher amount of summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c), which was also shown in the study of Park et al (2013). Strain WS-MY9 T contained phosphatidylethanolamine (PE) and two Naphthol-AS-BI-phosphohydrolase…”

“…The second species of the genus Algibacter, Algibacter mikhailovii, was subsequently described following isolation from a sea urchin (Nedashkovskaya et al, 2007). Recently, Algibacter aquimarinus has been described and Algibacter pectinivorans reclassified as a member of the genus Algibacter (Park et al, 2013). In this study, a novel bacterial strain, designated WS-MY9 T and isolated from brown algae collected from a reservoir located on Wando, an island of South Korea, is described.…”

Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand

“…Aquimarina addita JC2680 T (HM475137) with the one formed by the genera Hyunsoonleella (Yoon et al, 2010) and Jejuia (Lee et al, 2009), within a cluster encompassing the genera Hyunsoonleella, Jejuia and Marinivirga and two species of the genus Algibacter (Park et al, 2013b). Topologies of the maximum-parsimony and maximum-likelihood trees were nearly identical to the one shown by the neighbour-joining tree (Figs S1 and S2 available in IJSEM Online).…”

Algibacter agarivorans sp. nov. and Algibacter agarilyticus sp. nov., isolated from seawater, reclassification of Marinivirga aestuarii as Algibacter aestuarii comb. nov. and emended description of the genus Algibacter

“…The type species Algibacter lectus and the species Algibacter mikhailovii (Nedashkovskaya et al 2007) included strains from seaweed or strains that showed agarolytic activity. Recently, we reclassified a non-agarolytic marine bacterium Pontirhabdus pectinivorans (Yi et al, 2011) in this genus as Algibacter pectinivorans and described a novel species of the genus, Algibacter aquimarinus (Park et al, 2013b). During a study on the microbial diversity of the seawater of Gwangyang Bay, two yellow-pigmented flavobacterial strains, designated KYW560…”