Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand

Abstract

A yellow, rod-shaped, Gram-negative, facultatively aerobic, gliding bacterium, designed strain P7-3-5T, was isolated from intertidal sand of the Yellow Sea, China. Analysis of 16S rRNA gene sequences revealed that strain P7-3-5T formed a distinct lineage within the family Flavobacteriaceae , sharing 94.2–96.9 % sequence similarity with type strains of species of the most closely related genera, including Hyunsoonleella , Jejuia , Marinivirga and Algibacter . The strain grew at 4–40 °C and with 0.5–5.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed gelatin and DNA. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0 and the major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine (PE), three unidentified aminolipids (AL1–3) and four unidentified lipids (L1–4). The genomic DNA G+C content of strain P7-3-5T was 32.1 mol%. Data from this polyphasic study suggest that strain P7-3-5T represents a novel species in a new genus in the family Flavobacteriaceae , for which the name Arenitalea lutea gen. nov., sp. nov. is proposed. The type strain of Arenitalea lutea is P7-3-5T ( = CGMCC 1.12213T = KACC 16457T).

Planktosalinus lacus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a salt lake

“…Glycolipids and sphingolipids were not detected (data not shown). The presence of PE and an uncharacterized lipid as the major lipids was in agreement with the results reported for members of the genera Aequorivita (Liu et al, 2013) and Salinimicrobium Subhash et al, 2014). However, strain X14M-14 T could still be distinguished from all members of the genus Salinimicrobium by the presence of a second unknown lipid as a major polar lipid (Table 1) and from some members of the genus Aequorivita by the absence of some minor polar lipids.…”

“…The detailed fatty acid profiles of strain X14M-14 T and the type strains of species of the most closely related genera are shown in Table 2. It is noted that the fatty acid profiles obtained in this study for the reference strains were similar to those given in the original descriptions (Chen et al, 2008;Liu et al, 2013;Ying et al, 2007) in terms of the major fatty acids, despite some differences in their proportions. The major fatty acids of strain X14M-14 T were iso-C 15 : 0 (23.6 %), iso-C 15 : 1 G (21.8 %) and iso-C 17 : 0 3-OH (20.1 %).…”

“…The data obtained in this study for reference strains Aequorivita viscosa CGMCC 1.11023 T and S. catena CGMCC 1.6101 T are included in Table 1 and corresponded to the original descriptions (Liu et al, 2013;Ying et al, 2007).…”

“…The major fatty acids of strain X14M-14 T were iso-C 15 : 0 (23.6 %), iso-C 15 : 1 G (21.8 %) and iso-C 17 : 0 3-OH (20.1 %). The presence of iso-C 15 : 1 G as a major fatty acid for strain X14M-14 T was different from all members of the most closely related genera: Aequorivita (Bowman & Nichols, 2002;Liu et al, 2013;Park et al, 2009) and Salinimicrobium (Chen et al, 2008;Lee et al, 2012;Lim et al, 2008;Nedashkovskaya et al, 2010;Subhash et al, 2014;Ying et al, 2007). The lack of summed features 3 (C 16 : 1 v7c and/or C 16 : 1 v6c) and 9 (iso-C 17 : 1 v9c and/or C 16 : 0 10-methyl) in strain X14M-14 T could differentiate strain X14M-14 T clearly from the type strains of species of the genera Aequorivita and Salinimicrobium (Table 2).…”

“…At the time of writing, this family consisted of about 120 genera with validly published names, most members of which are characterized as aerobic, having rod-shaped cells and containing MK-6 as the major respiratory quinone (McBride, 2014). Members of the family Flavobacteriaceae are distributed globally in various environments, such as freshwater (Zhang et al, 2014), seawater (Park et al, 2013), sediment , sand (Zhang et al, 2013), plant tissue (Liu et al, 2013), fish (Shakeela et al, 2015) and so on. During a survey on the bacterial diversity of a salt lake (Lake Xiaochaidan), a novel Flavobacteriaceae-like strain, designated X14M-14 T , was obtained.…”