“…Currently there are over fifty companies that produce some types of aquatic biomass either for use as supplement, additives, colorants, food or bioenergy (Klöck, 2010 In the production of bioenergy from aquatic biomass, as in any types of biorefineries, a very important step is the pretreatment of the raw materials, where the objective is to make available the intracellular compounds such as carbohydrates, proteins, lipids, and added-value compounds such as short chain oligosaccharides, antioxidants, pigments, etc. (Harun et al, 2014;Demuez et al, 2015). The two main objectives for which pretreatment of microalgae biomass is necessary are the breakdown of the cell wall and the modification of the structure of intracellular carbohydrates.…”