Aldosterone and renin measurements

Cartledge, Susan; Lawson, Nigel

doi:10.1258/0004563001899401

Cited by 63 publications

(39 citation statements)

References 88 publications

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“…Plasma renin activity is determined by adding exogenous AGT or TDP to a plasma sample and measuring Ang I formation by radioimmunoassay. 27,28 Sensitivity is a requirement of the renin assay because normal plasma renin activity concentrations are low and subnormal concentrations have diagnostic relevance. 27 Because of volume requirements of the classical methods and because of increasing use of mice in experimental studies, there was a need for more sensitive and specific ACE and renin assays.…”

Section: Discussionmentioning

confidence: 99%

Novel Mass Spectrometric Methods for Evaluation of Plasma Angiotensin Converting Enzyme 1 and Renin Activity

2005

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Abstract-This article demonstrates the applicability of quantitative proteomics to assays of proteolytic enzyme activity.A novel assay was developed for measurement of renin and angiotensin-converting enzyme (ACE) activity in plasma.The method was validated in animal models associated with alterations of the renin angiotensin system (RAS). Using surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) with a ProteinChip Array technology, plasma renin and ACE1 could be measured in Ͻ0.5 L of plasma. Plasma is incubated with peptide substrates for renin and ACE, tetradecapeptide (TDP), and angiotensin I (Ang I), respectively. The reactions mixtures are spotted onto the ProteinChip WCX2 and detected using SELDI-TOF-MS. Peak height or area under curve for TDP, Ang I, and angiotensin II (Ang II) peaks are measured. There was a linear relationship between disappearance of substrate and appearance of products for both renin and ACE (R 2 ϭ0.95 to 0.98). ACE1 activity was blocked with chelating agents (EDTA and 1,10 phenanthrolene), indicating action of a metalloprotease. The ACE1 inhibitor, captopril, selectively blocked ACE1. Renin activity was specifically blocked with renin inhibitor and was not affected by phenanthrolene or captopril. Animal models tested were Ang AT1a receptor-deficient and streptozotocin (STZ) diabetic mice. Plasma renin activity was increased Ͼ2-fold in AT1aϪ/Ϫ as compared with AT1a ϩ/ϩ . In STZ diabetic mice, ACE1 was increased 2-fold as compared with controls. The advantage of the method is that it is tagless, does not require additional purification steps, and is extremely sensitive. The approach can be multiplexed and used for identification of novel substrates/inhibitors of the RAS.

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Section: Discussionmentioning

confidence: 99%

Novel Mass Spectrometric Methods for Evaluation of Plasma Angiotensin Converting Enzyme 1 and Renin Activity

2005

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“…Measurement of PRC instead of PRA may reduce some preanalytical and analytical problems and, therefore, improve intra-and interlaboratory reproducibility (23,24 ). Major advantages of the new PAC and PRC assays evaluated in our study include the ease and performance of the test (no cooling, a single plasma sample, and good interlaboratory reproducibility because the assays are automated) and the rapid availability of results (automated immunochemiluminometric assays), which is a prerequisite for extensive screening of hypertensive patients for PHA.…”

Section: Discussionmentioning

confidence: 99%

Rapid Screening Test for Primary Hyperaldosteronism: Ratio of Plasma Aldosterone to Renin Concentration Determined by Fully Automated Chemiluminescence Immunoassays

Perschel¹,

Schemer

Seiler

et al. 2004

Clinical Chemistry

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Background:The ratio of plasma aldosterone concentration to plasma renin activity (PAC/PRA) is the most common screening test for primary hyperaldosteronism (PHA), but it is not standardized among laboratories. We evaluated new automated assays for the simultaneous measurement of PAC and plasma renin concentration (PRC). Methods: We studied 76 healthy normotensive volunteers and 28 patients with confirmed PHA. PAC and PRC were measured immunochemically in EDTA plasma on the Nichols Advantage ® chemiluminescence analyzer, and PRA was determined by an activity assay. Results: In volunteers, PAC varied from 33.3 to 1930 pmol/L, PRA from 1.13 to 19.7 ng ⅐ mL

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“…The stability of steroids including aldosterone in sera stored at Ϫ20°C or lower has been previously established. 27 …”

Section: Measurement Of Serum Aldosteronementioning

confidence: 99%

Relations of Serum Aldosterone to Cardiac Structure

et al. 2004

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Abstract-Aldosterone is associated with myocardial fibrosis in experimental studies and with left ventricular remodeling in heart failure patients. We hypothesized that aldosterone influences ventricular remodeling in people without congestive heart failure in the community. We examined the relations between serum aldosterone and echocardiographic left ventricular measurements in 2820 Framingham Study subjects (mean age 57 years, 58% women, 88% white) free of myocardial infarction and overt heart failure. Serum aldosterone levels were higher in women compared with men.In linear regression models (adjusted for age, systolic blood pressure, weight, height, diabetes, heart rate, hypertension treatment, and ethnicity), left ventricular wall thickness and relative wall thickness were positively related, and left ventricular diastolic dimensions were inversely related to serum aldosterone in women (PϽ0.05 for all), but not in men (PϾ0.20 for all). There was no effect modification of the relations observed in women by menopausal status. The gender-related differences in relations of serum aldosterone to relative wall thickness were consistent across subgroups defined on the basis of sex-specific median values of systolic blood pressure and body mass index. Fractional shortening, left ventricular mass, and left atrial dimensions were not related to serum aldosterone in either sex. In conclusion, in our community-based sample of individuals free of myocardial infarction and heart failure, serum aldosterone was positively associated with a left ventricular geometric pattern suggestive of concentric remodeling (increased left ventricular wall thickness and relative wall thickness but decreased internal dimensions) in women but not in men. Additional investigations are warranted to confirm these findings. Aldosterone has been demonstrated to influence LV remodeling independent of its impact on systemic blood pressure (BP). 5 These experimental studies are paralleled by clinical reports demonstrating that serum aldosterone levels are elevated in patients with heart failure and denote an adverse prognosis. 6 Furthermore, treatment with aldosterone antagonists results in reduced mortality in heart failure patients 7 and favorable changes in LV remodeling. 8,9 The aforementioned observations have stimulated interest in the putative role of aldosterone in LV remodeling in the absence of heart failure. 10 -15 Primary hyperaldosteronism and systemic hypertension are associated with LV hypertrophy that is reversed by adrenal surgery and treatment with aldosterone antagonists, respectively. 10 -17 However, other reports examining the association of serum aldosterone with LV remodeling in individuals without heart failure have yielded inconsistent results. 18 -22 For instance, one report underscored that serum aldosterone was associated with LV mass (LVM) in obese blacks, but not in nonobese blacks or in whites (irrespective of body mass). 21 Another report emphasized an association of serum aldosterone with LV geometric pattern, bu...

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Aldosterone and renin measurements

Abstract: This article was prepared at the invitation of the Analytical Investigations Standing Committee of the Association of Clinical Biochemists.

Cited by 63 publications

References 88 publications

Novel Mass Spectrometric Methods for Evaluation of Plasma Angiotensin Converting Enzyme 1 and Renin Activity

Novel Mass Spectrometric Methods for Evaluation of Plasma Angiotensin Converting Enzyme 1 and Renin Activity

Rapid Screening Test for Primary Hyperaldosteronism: Ratio of Plasma Aldosterone to Renin Concentration Determined by Fully Automated Chemiluminescence Immunoassays

Relations of Serum Aldosterone to Cardiac Structure

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