Alcoholic Extract of Wrightia tinctoria leaves and Isolates there of afford DNA Protection in Post Irradiated Sprague Dawley Rats

Abstract

Objective: The current study investigates the potential of alcoholic extract of Wrightia tinctoria leaves and its isolates in repairing the DNA damage induced by gamma radiation. Methodology: The ethanolic extract of Wrightia tinctoria (EEWT) the leaves was subjected to in vitro anti oxidant assay by DPPH radical scavenging and hydrogen peroxide scavenging methods. He EEWT was investigated to the Micronucleus assay, spleen colony assay and comet assay in Sprague Dawley rats wherein DNA damage had already been induced by irradiation. Results: The ethanolic extract of Wrightia tinctoria leaves having good antioxidant activity. In the micro nucleus assay, a 2 Gy quantum of wholebody gamma radiation significantly enhanced the levels of micronucleated reticulocytes and this induction was substantially reversed in the extract treatment group. Results of the spleen colony assay pointed to the extract's ability to enhance the recovery of the hematopoietic system, in a dosedependent manner, following 6 Gy gamma irradiation. The decrease in cellular repair index due to enhanced DNA repair was evident from the comet assay parameters. Moreover, the isolates from ethanolic extract of Wrightia tinctoria, when imperiled to DNA protection by in vitro gamma radiation method in plasmid pBR322 DNA, abetted DNA repair at higher dose. Conclusion: The DNA protection afforded is likely attributable to free radical scavenging properties along with a potential for regenerating the immune system through repair of post irradiated DNA.

Effects of Gamma Irradiation on Microbiological, Phytochemical Content, Antioxidant Activity and Inhibition of Angiotensin Converting Enzyme (ACE) Activity of Peperomia pellucida (L.) Kunth

“…Irradiation treatment damaged microbial cells by break down the DNA, photons induced the death of the organism and caused some organisms can not reproduce. 4,15 There are several reports on effect of gamma irradiation on total phenolic content (TPC) and total flavonoid content (TFC). The gamma irradiation increased TPC in clove, nutmeg and skin of almond after irradiation.…”

Utility of Isatin Semicarbazones in Mammary Carcinoma Cells - A Proof of Concept Study

“…Antioxidant activity by DPPH method A 0.1 mM solution of DPPH in methanol 15,16 was prepared and 1 ml of this solution was added to 3 ml of sample solution in water at different concentrations. 17 This mixture was vortexed and kept in the dark for equilibration at 25°C.…”