Alcohol oxidase protein mediated in-situ synthesized and stabilized gold nanoparticles for developing amperometric alcohol biosensor

Chinnadayyala, Somasekhar R.; Santhosh, Mallesh; Singh, Naveen K.; Goswami, Pranab

doi:10.1016/j.bios.2015.02.015

Cited by 53 publications

(31 citation statements)

References 39 publications

(35 reference statements)

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“…For example, the spectrum of gPdNPs has a peak at 390 nm (Figure 1), which is specific for PdNPs [18,61]. A peak of gAuNPs in the range 525-590 nm is specific for chemically-synthesized AuNPs of different sizes, according to data reported in the literature [8,12,13,19,38,42,43,48] as well as our results [56,62]. The color, size and shape of AuNPs and gAuNPs are dependent on the NPs' synthesis conditions: culture liquid of a 1-day culture incubated for 3 days with HAuCl 4 produces a dark blue solution of small gAuNPs with a peak at 525 nm.…”

Section: Characterization Of the Green-synthesized Nanoparticlessupporting

confidence: 63%

“…Many plants [11,12,21,[24][25][26], algae [4], microorganisms [1,3,[7][8][9][10]14,18,23,[27][28][29][30], as well as redox-imbalanced mammalian cells and systems [31] are known to produce nanostructured mineral crystals and metallic NPs with properties similar to chemically-synthesized materials. Biological agents, including polysaccharides, polypeptides, DNA, enzymes secreted by cells [24,[32][33][34][35][36][37][38][39][40][41] and purified enzymes [42,43] are able to reduce noble metal ions to gain metallic NPs. Besides the practical aim of gNPs production, investigations on the green synthesis processes will be useful for elucidating the mechanisms of cells' responses to stress caused by metal ions.…”

Section: Introductionmentioning

confidence: 99%

“…The exact mechanism of gNP synthesis using biological agents has not been elucidated to date. However, it has been suggested that various biomolecules are involved in this process [24,[38][39][40][41][42][43][44][45][46][47][48]. Capping and stabilizing agents (polysaccharides, polypeptides and other bioorganic compounds) prevent the gNPs from further growth and agglomeration in resulted colloidal solution [45][46][47][48][49][50][51].…”

Section: Introductionmentioning

confidence: 99%

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Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

et al. 2019

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Novel nanomaterials, including metallic nanoparticles obtained via green synthesis (gNPs), have a great potential for application in biotechnology, industry and medicine. The special role of gNPs is related to antibacterial agents, fluorescent markers and carriers for drug delivery. However, application of gNPs for construction of amperometric biosensors (ABSs) is not well documented. The aim of the current research was to study potential advantages of using gNPs in biosensorics. The extracellular metabolites of the yeast Ogataea polymorpha were used as reducing agents for obtaining gNPs from the corresponding inorganic ions. Several gNPs were synthesized, characterized and tested as enzyme carriers on the surface of graphite electrodes (GEs). The most effective were Pd-based gNPs (gPdNPs), and these were studied further and applied for construction of laccase- and alcohol oxidase (AO)-based ABSs. AO/GE, AO-gPdNPs/GE, laccase/GE and laccase-gPdNPs/GE were obtained, and their analytical characteristics were studied. Both gPdNPs-modified ABSs were found to have broader linear ranges and higher storage stabilities than control electrodes, although they are less sensitive toward corresponding substrates. We thus conclude that gPdNPs may be promising for construction of ABSs for enzymes with very high affinities to their substrates.

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Section: Characterization Of the Green-synthesized Nanoparticlessupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

et al. 2019

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“…of aggregation [8][9][10][11][12][13][14][15][16][17][18]. Several evidences have shown the formulation of nanoparticles using different small biomolecules like monosaccharides (glucose or galactose), amino acids, short peptides isolated from plant [8][9][10][11][12][13][14][15][16][17][18]. Complex biomolecules like long chain peptides and microorganisms have also been used efficiently for reduction of metal ions to its nano form and this nano-conjugates have also been found to have significant potential in therapeutics [14][15][16][17].…”

Section: Green Peptide-nanomaterials; a Friendly Healing Touch For Skmentioning

confidence: 99%

Green Peptide–nanomaterials; A Friendly Healing Touch for Skin Wound Regeneration

et al. 2019

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The complex phenomenon by which the body responds to any injury of skin or tissue is known as wound healing. A number of phases like exudative, proliferative, and extracellular matrix remodeling are orchestrated events to be occurred involving blood cells, parenchymal cells, and different soluble mediators. Different internal, as well as external factors, regulate the speed and quality of healing. The delay in wound healing process causes the chronic wound or scar formation. At the present moment, the upscale research for identification of agents causing accelerated healing is important. Moreover, the biocompatibility of the accelerators needs to be investigated. Recent biomedical researches for wound care target to provide antimicrobial protection as well as matrix scaffolding for quick repairing of the skin tissue. In recent studies with natural peptides have shown that they are important components in developing the nano-medicines for their usefulness and therapeutic efficiency. New therapeutic formulations can be developed using these natural peptides utilizing different nanoparticle delivery system. This review deals with the developmental study on efficient wound care system where the possible use of natural peptides in combination with nanomaterials has been explored. A trial has also been made on the findings made over the past few years on the use of peptides as tissue regenerating agents through effective wound healing pathway.

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“…[11], as a bio-recognition element for ethanol sensing [1][2][3][7][8][9][10][11] and development of biofuel cells [4][5][6], has been the focal point of many studies in the last years. It is oligomeric enzyme consisting of eight identical subunits, each containing a tightly bound flavin adenine dinucleotide (FAD) redox center [12].…”

Section: Introductionmentioning

confidence: 99%

Bioelectrocatalytic Activity of Immobilized Alcohol Oxidase on 4‐(pyrrole‐1‐yl) Benzoic Acid Modified Carbon Nanotubes

Kowalewska

Jakubow

2016

Electroanalysis

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A novel bioelectrocatalytic system was prepared by immobilizing alcohol oxidase (AOx) onto multiwalled carbon nanotubes (MWCNT) modified with 4‐(pyrrole‐1‐yl) benzoic acid (PyBA). Functional carboxylic groups from PyBA create covalent amide linkages with amine groups from the enzyme molecule and provide an anchor for the effective immobilization of AOx improving the stability of the whole system. The immobilized enzyme displayed a pair of reversible redox peaks of flavin adenine dinucleotide (FAD) cofactor with the formal potential E0’=−0.451 V. The response showed a surface‐controlled electrode process with the heterogeneous electron transfer rate constant ks=2.7 s−1. Under aerobic conditions AOx(FADH2) can be oxidized to AOx(FAD) by oxygen, which then reacts with ethanol decreasing the cathodic response, which could be used for ethanol detection with a high sensitivity 13.1 μA mM−1 cm−2. The lack of bioactivity towards ethanol in anaerobic conditions suggests the presence of two types of AOx molecules in the system: active with oxygen maintaining the direct electron transfer feature and not active without a redox mediator, due to the deeply embedded FAD cofactor. The polarization curve showed that the electrooxidation current of ethanol appears at −410 mV and reaches 2.0 µA cm−1 at −300 mV. In this case, the bioactivity of AOx to ethanol can be observed offering promising solution for the development of mediatorless systems for application to biosensors and biofuel cells.

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Alcohol oxidase protein mediated in-situ synthesized and stabilized gold nanoparticles for developing amperometric alcohol biosensor

Cited by 53 publications

References 39 publications

Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

Green Peptide–nanomaterials; A Friendly Healing Touch for Skin Wound Regeneration

Bioelectrocatalytic Activity of Immobilized Alcohol Oxidase on 4‐(pyrrole‐1‐yl) Benzoic Acid Modified Carbon Nanotubes

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