Alcohol dehydrogenase 5 of Helicoverpa armigera interacts with the CYP6B6 promoter in response to 2‐tridecanone

Abstract

Alcohol dehydrogenase 5 (ADH5) is a member of medium‐chain dehydrogenase/reductase family and takes part in cellular formaldehyde and S‐nitrosoglutathione metabolic network. 2‐tridecanone (2‐TD) is a toxic compound in many Solanaceae crops to defend against a variety of herbivory insects. In the broader context of insect development and pest control strategies, this study investigates how a new ADH5 from Helicoverpa armigera (HaADH5) regulates the expression of CYP6B6, a gene involved in molting and metamorphosis, in response to 2‐TD treatment. Cloning of the HaADH5 complementary DNA sequence revealed that its 1002 bp open reading frame encodes 334 amino acids with a predicted molecular weight of 36.5 kD. HaADH5 protein was purified in the Escherichia coli Transetta (pET32a‐HaADH5) strain using a prokaryotic expression system. The ability of HaADH5 protein to interact with the 2‐TD responsive region within the promoter of CYP6B6 was confirmed by an in vitro electrophoretic mobility shift assay and transcription activity validation in yeast. Finally, the expression levels of both HaADH5 and CYP6B6 were found to be significantly decreased in the midgut of 6th instar larvae after 48 h of treatment with 10 mg/g 2‐TD artificial diet. These results indicate that upon 2‐TD treatment of cotton bollworm, HaADH5 regulates the expression of CYP6B6 by interacting with its promoter. As HaADH5 regulation of CYP6B6 expression may contribute to the larval xenobiotic detoxification, molting and metamorphosis, HaADH5 is a candidate target for controlling the growth and development of cotton bollworm.

Adaptation by copy number variation increases insecticide resistance in the fall armyworm

“…CYP9A12 and CYP9A14 were overexpressed in a pyrethroid-resistant strain of Helicoverpa armigera 36 , and functional expression of these two CYP genes demonstrated their ability to metabolize pyrethroids 37 . In H. armigera, ADH5 binds a promoter of CYP6B6 in the response of xenobiotic 2-tridecanone 38 . Deciphering the functions of CYP and ADH genes from scaffold 17 will contribute to understanding insecticide resistance in PR.…”

Analysis of Genes Associated with Feeding Preference and Detoxification in Various Developmental Stages of Aglais urticae

“…In addition to the genes analyzed through GO, Alcohol dehydrogenase 5 (ADH5), CYP3s, cathepsin B (cat-B), SUMF1, and Heat shock protein (HSP) genes were also found to be involved in the detoxification process in the signaling pathway of "Drug metabolism cytochrome P450" and "Insect hormone biosynthesis". CYP3, HSP, cat-B, and ADH5 genes are essential in detoxifying exogenous toxic substances [36].…”