Regulation of insect P450s in response to phytochemicals

Li, Xianchun; Deng, Zhongyuan; Chen, Xuewei

doi:10.1016/j.cois.2020.12.003

Cited by 45 publications

(53 citation statements)

References 69 publications

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“…Many P450s that are involved in allelochemical detoxification are also responsible for insecticide resistance [57]. Expression of insect P450 genes is constitutively low and phytochemical-inducible [58]. More importantly, many insects can utilize these plant defensive compounds as inducers to enhance detoxification capacity [59][60][61].…”

Section: Discussionmentioning

confidence: 99%

Exposure of Helicoverpa armigera Larvae to Plant Volatile Organic Compounds Induces Cytochrome P450 Monooxygenases and Enhances Larval Tolerance to the Insecticide Methomyl

Ding

Shi

et al. 2021

Insects

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Plants release an array of volatile chemicals into the air to communicate with other organisms in the environment. Insect attack triggers emission of herbivore-induced plant volatiles (HIPVs). How insect herbivores use these odors to plan their detoxification systems is vital for insect adaptation to environmental xenobiotics. Here we show that the larvae of Helicoverpa armigera (Hübner), a broadly polyphagous lepidopteran herbivore, have the capacity to use plant volatiles as cues to upregulate multiple detoxification systems, including cytochrome P450 monooxygenases (P450s), for detoxification of insecticides. Olfactory exposure of the fifth instars to two terpene volatiles limonene and nerolidol, and two green-leaf volatiles 2-heptanone and cis-3-hexenyl acetate significantly reduced larval susceptibility to the insecticide methomyl. However, larval pretreatment with piperonyl butoxide (PBO), a known P450 inhibitor, neutralized the effects of volatile exposure. Furthermore, larval exposure to the four plant volatiles enhanced activities of P450 enzymes in midguts and fatbodies, and upregulated expression of CYP6B2, CYP6B6 and CYP6B7, P450s involved in detoxification of the insecticide. Larval exposure to 2-heptanone and limonene volatiles also enhanced activities of glutathione-s-transferase and carboxylesterase. Our findings suggest that olfactory exposure to HIPVs enhances larval insecticide tolerance via induction of detoxification P450s.

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Section: Discussionmentioning

confidence: 99%

Exposure of Helicoverpa armigera Larvae to Plant Volatile Organic Compounds Induces Cytochrome P450 Monooxygenases and Enhances Larval Tolerance to the Insecticide Methomyl

Ding

Shi

et al. 2021

Insects

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“…Although numerous studies demonstrate Cyp4p1 , Cyp6a8 , or Cyp6d5 induction in Drosophila , to our knowledge only one has directly demonstrated that the xenobiotic is modified or degraded by the induced CYP 25 . More generally, the signal transduction pathway by which xenobiotics induce CYP expression is poorly understood in insects 26 . As a result, it is possible that xenobiotic exposure leads to the activation of transcription factors that increase expression of a broad array of CYPs, not all of which target the xenobiotic.…”

Section: Discussionmentioning

confidence: 99%

“…In Drosophila as in other insects, the presence of xenobiotics leads to the transcriptional upregulation of selective CYPs, a process referred to as induction 22 – 26 . Although CYP induction is generally measured following xenobiotic feeding or physical contact, some recent studies in beetles, locusts, and moths have indicated that exposure to volatile odorants can induce CYP expression in the antenna 27 – 30 .…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of CYPs induced in the Drosophila antenna by exposure to a plant odorant

Baldwin

Mohapatra

Nagalla

et al. 2021

Sci Rep

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Members of the cytochrome p450 (CYP) enzyme family are abundantly expressed in insect olfactory tissues, where they are thought to act as Odorant Degrading Enzymes (ODEs). However, their contribution to olfactory signaling in vivo is poorly understood. This is due in part to the challenge of identifying which of the dozens of antennal-expressed CYPs might inactivate a given odorant. Here, we tested a high-throughput deorphanization strategy in Drosophila to identify CYPs that are transcriptionally induced by exposure to odorants. We discovered three CYPs selectively upregulated by geranyl acetate using transcriptional profiling. Although these CYPs are broadly expressed in the antenna in non-neuronal cells, electrophysiological recordings from CYP mutants did not reveal any changes in olfactory neuron responses to this odorant. Neurons were desensitized by pre-exposing flies to the odorant, but this effect was similar in CYP mutants. Together, our data suggest that the induction of a CYP gene by an odorant does not necessarily indicate a role for that CYP in neuronal responses to that odorant. We go on to show that some CYPs have highly restricted expression patterns in the antenna, and suggest that such CYPs may be useful candidates for further studies on olfactory CYP function.

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“…One the other hand, MAPK genes have been speculated to mediate the balanced rise of both juvenile hormone (JH) and molting hormone (20E), leading to reduction in the fitness cost incurred by Cry1Ac resistance in the diamondback moth [33]. Additionally, the generation of ROS and activation of MAPK members are both observed in insects under xenobiotic stress [75,76], suggesting that the ROS-scavenging system and MAPK-dependent pleiotropic hormone signaling network may work together to minimize the fitness costs of insecticide resistance. Further research is needed to reveal if the 2 modifier systems coexist and how they are orchestrated to reduce fitness cost when both modifier systems are present.…”

Section: Plos Biologymentioning

confidence: 99%

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

et al. 2021

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Chemical insecticides have been heavily employed as the most effective measure for control of agricultural and medical pests, but evolution of resistance by pests threatens the sustainability of this approach. Resistance-conferring mutations sometimes impose fitness costs, which may drive subsequent evolution of compensatory modifier mutations alleviating the costs of resistance. However, how modifier mutations evolve and function to overcome the fitness cost of resistance still remains unknown. Here we show that overexpression of P450s not only confers imidacloprid resistance in the brown planthopper, Nilaparvata lugens, the most voracious pest of rice, but also leads to elevated production of reactive oxygen species (ROS) through metabolism of imidacloprid and host plant compounds. The inevitable production of ROS incurs a fitness cost to the pest, which drives the increase or fixation of the compensatory modifier allele T65549 within the promoter region of N. lugens peroxiredoxin (NlPrx) in the pest populations. T65549 allele in turn upregulates the expression of NlPrx and thus increases resistant individuals’ ability to clear the cost-incurring ROS of any source. The frequent involvement of P450s in insecticide resistance and their capacity to produce ROS while metabolizing their substrates suggest that peroxiredoxin or other ROS-scavenging genes may be among the common modifier genes for alleviating the fitness cost of insecticide resistance.

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Regulation of insect P450s in response to phytochemicals

Cited by 45 publications

References 69 publications

Exposure of Helicoverpa armigera Larvae to Plant Volatile Organic Compounds Induces Cytochrome P450 Monooxygenases and Enhances Larval Tolerance to the Insecticide Methomyl

Exposure of Helicoverpa armigera Larvae to Plant Volatile Organic Compounds Induces Cytochrome P450 Monooxygenases and Enhances Larval Tolerance to the Insecticide Methomyl

Identification and characterization of CYPs induced in the Drosophila antenna by exposure to a plant odorant

Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

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