Alcian Blue 8 G With Chlorantine Fast Red 5 B. A Technic for Selective Staining of Mucopolysaccharides

Lison, L

doi:10.3109/10520295409115457

Cited by 204 publications

(46 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This implies that acid mucopolysaccharides are present in the main body of the tunic but are absent from the cuticle and refringent granules, a result which is in agreement with Peres's description of the former as differing from the latter in staining metachromatically with toluidine blue (presumably gamma metachromasia). He also uses the term 'glycoprotein' in this context, but in current terminology a positive alcian blue response and gamma metachromasia are held to be an indication specifically of acid mucopolysaccharides (Lison, 1953;Pearse, 1954)· With the PAS test, which was not used by Peres, a positive reaction is-given by the cuticle and by the refringent granules; in the rest of the tunic the fibres are positive, and there is a good deal of fine granulation which reacts in the same way, while the general matrix gives a very faint coloration. It seems clear from this response of the cuticle that it cannot be composed of pure protein, as Peres supposed.…”

mentioning

confidence: 99%

On the organic binding of iodine in the tunic ofCiona intestinalisL.

Barrington

Barron

1960

J. Mar. Biol. Ass.

View full text Add to dashboard Cite

It is now known that organic binding of iodine takes place in the endostyle of Ciona (Barrington, 1957) and Amphioxus (Thomas, 1956; Barrington, 1958), and there isevidence that at least di-iodotyrosine and thyroxine are formed as a result of this processin Ciona (Barrington, 1959; Robertson, personal communication. Since this biosynthesis appears to be a specialized property of a restricted group of cells, it has been suggested (Barrington, 1959) that it is not a chance by-product of endostylar secretion but that the thyroidal products are a biochemical necessity for the animals concerned. Attention has been drawn also to the occurrence of iodine binding at the surface of the tunic (or test) of Ciona (Barrington, 1957), and it is the purpose of the present paper to present some analysis of this latter situation and to enquire as to its possible relationship with the endostylar activity.

show abstract

mentioning

confidence: 99%

On the organic binding of iodine in the tunic ofCiona intestinalisL.

Barrington

Barron

1960

J. Mar. Biol. Ass.

View full text Add to dashboard Cite

show abstract

“…Schultz's cholesterol stain, hematoxylin and eosin stains and Von Kossa calcium stain were conducted as outlined by Armed Forces Institute of Pathology (1968). Collagen and acid mucopolysaccharides (AMPS) were stained by the method of Lison (1954) as modified by Zugibe, Brown and Last (1959), i.e., alcian blue was mixed with hydrochloric acid rather than acetic acid.…”

Section: Methodsmentioning

confidence: 99%

Atherosclerosis in Ruminants

Wiggers

Jacobson

Getty

1971

Journal of Animal Science

View full text Add to dashboard Cite

“…The PAS reaction (counterstained with Harris's haematoxylin, followed either by metanil yellow or by tartrazine) has therefore been used (controlled by acetylation) to demonstrate the presence of 1:2-glycol groups, and toluidine blue metachromasia and the a1cian blue method of Steedman (1950) to show whether or not the reacting secretion contains acid mucopolysaccharides, the importance of the latter being that they appear to be the main components of epithelial mucins (Pearse, 1954). The selectivilj of the alcian blue was found to be much improved by the addition of an equal volume of 1% acetic acid to the 1% solution of the dye, as suggested by Lison (1954). The classical mucicarmine technique of Mayer, using Southgate's method of preparing the staining solution, has also been valuable, as has the Azan procedure.…”

Section: Methodsmentioning

confidence: 99%

The Localization of Organically Bound Iodine in the Endostyle of Amphioxus

Barrington

1958

J. Mar. Biol. Ass.

View full text Add to dashboard Cite

An account is given of some features of the organization of the endostyle of Amphioxus, with particular reference to the distribution of sites of mucus secretion as indicated by positive responses to tests for acid mucopolysaccharides. Autoradiography shows that the centre of distribution of organically-bound iodine is associated with the tips of the mucus-secreting cells of zone 5, and not with the glandular tracts. Mucus secretion also occurs elsewhere in the endostyle (although not in the dorsal glandular tracts), but as it is not associated with any accumulation of bound iodine it is concluded that the binding in zone 5 is a specialized property of the cells of that particular zone. The results are discussed in the light of recent work on the protochordates and the ammocoete larva, and it is concluded that in Amphioxus, as in the Tunicata, there is reason for supposing that the iodination process is a biochemical specialization and that its product must therefore be of some physiological significance to the organism. Attention is also drawn to the evolutionary interest of the association of iodine-binding with a mucin rather than with a glycoprotein.

show abstract

Alcian Blue 8 G With Chlorantine Fast Red 5 B. A Technic for Selective Staining of Mucopolysaccharides

Cited by 204 publications

References 6 publications

On the organic binding of iodine in the tunic ofCiona intestinalisL.

On the organic binding of iodine in the tunic ofCiona intestinalisL.

Atherosclerosis in Ruminants

The Localization of Organically Bound Iodine in the Endostyle of Amphioxus

Contact Info

Product

Resources

About