Albumin-Based Lipoprotein Nanoparticles for Improved Delivery and Anticancer Activity of Curcumin for Cancer Treatment

Abstract

Aim: To prepare curcumin (CUR)-loaded, dioleoyl phosphoethanolamine-conjugated human serum albumin nanoparticles (NPs) and to evaluate their effectiveness in breast cancer therapy. Materials & methods: The CUR-loaded NPs were physicochemically characterized and evaluated for their cytotoxicity in murine (4T1) and human breast cancer (MDA-MB-231) cell lines. The antitumor efficacy of the nanomedicine was evaluated in 4T1 tumor bearing mice. Results: The prepared NPs exhibited encapsulation and drug loading efficiencies of approximately 79 and 21%, respectively. The NPs were taken up efficiently and markedly hindered the proliferation of breast cancer cells compared with free drug. NPs exhibited greater suppression of tumor growth in 4T1 tumor bearing mice. Conclusion: CUR-human serum albumin-dioleoyl phosphoethanolamine NPs could be a potential treatment alternative for solid tumors, including breast cancer.

Molecular nanoinformatics approach assessing the biocompatibility of biogenic silver nanoparticles with channelized intrinsic steatosis and apoptosis

“…The synthesis of G-AgNPs was hypothesized to be mediated by the presence of biomolecules such as proteins, polysaccharides, and other molecules in aqueous plant extract. 13 Hence, to reveal the role of different biomolecules, GC-MS analysis was performed with pre and post-synthesis aqueous plant extracts. As shown in Table 2, the aqueous plant extract of silver grass indicated the presence of different biomolecules at different retention times.…”

“…Evaluation of biocompatibility of nanoparticles is an important criterion to determine their utility for biomedical and environmental purposes. 59 After being used, the nanoparticles can be released into the environment and come in contact with organisms, especially in the water. Hence, an aquatic model was chosen to determine the biocompatibility of G-AgNPs.…”

“…The exposed AgNPs (G-AgNPs and C-AgNPs) were hypothesized to exhibit biotoxicity through a channel of cellular processes initiated by the accumulation at the surface and subsequent uptake of nanoparticles. 13 The uptake of nanoparticles was estimated through flow cytometry analysis. The change in the granularity of cells has been described to be an important factor to assess the uptake of nanoparticles.…”

“…AgNPs have been reported to cause cytotoxicity by dysregulating the metabolic function through induction of oxidative stress. 13,64 Hence, it was postulated that internalized AgNPs were inducing oxidative stress by enhancing the production of ROS, which further causes imbalanced activities like lipid alteration and apoptosis. G-AgNPs and C-AgNPs were assessed for their potential to induce oxidative stress and anomaly in metabolic processes.…”

“…The exploration of toxicity in zebrafish embryos and adult zebrafish has revealed that AgNPs exhibits cytotoxicity at molecular and atomic levels by inducing oxidative stress and apoptosis as a consequence of their dose-dependent internalization that influences protein machinery through their molecular interaction and accumulation at the surface of cells. 13 Other metal and metal oxide nanoparticles have shown similar toxicity mechanisms. 5,14 However, knowledge about their initial interaction with membrane lipids leading to their internalization is limited.…”