Albumin adsorption from aqueous solutions and human plasma in a packed-bed column with Cibacron Blue F3GA-Zn(II) attached poly(EGDMA-HEMA) microbeads

Novel antifouling oligo(ethylene glycol) methacrylate particles via surfactant-free emulsion polymerization

“…6 presents the equilibrium BSA adsorption capacities of cross-linked PMMA and PEGEEM particles at pH 5.0 and 7.0. These pH values were chosen considering the isoelectric point of BSA (pI = 5.0) [6,43,44] and the physiological pH [45]. The equilibrium BSA adsorption capacities of PMMA particles are higher at pH 5.0 compared with pH 7.0.…”

“…The equilibrium BSA adsorption capacities of PMMA particles are higher at pH 5.0 compared with pH 7.0. At pH 5.0, BSA does not have a net electrical charge which causes hydrophobic interactions to become effective, resulting in maximum adsorption of protein molecules [43,44,[46][47][48]. On the other hand, at both pH values, cross-linked PEGEEM particles have no significant nonspecific BSA adsorption.…”

Overview of Albumin and Its Purification Methods

“…The biomimetic monocholoro or dicholorotriazinyl dyes are able to bind most type of proteins, especially enzymes. 87,88 It has so many advantages over other methods. 89 This method is economically and widely available and its immobilization and scale up is easy.…”

Bilirubin removal from human plasma by dye affinity microporous hollow fibers

“…It is reported that some sorbents like activated carbon can remove bilirubin only from the free or soluble phase, and the removal efficiency is limited by the tight binding of bilirubin to albumin (38). Starting from the same point, we selected Cibacron Blue F3GA as the affinity ligand, which was shown as a good ligand for affinity separation of albumin in our previous studies (39,40). Starting from the same point, we selected Cibacron Blue F3GA as the affinity ligand, which was shown as a good ligand for affinity separation of albumin in our previous studies (39,40).…”