Alaska Pollack Protein Prevents the Accumulation of Visceral Fat in Rats Fed a High Fat Diet

Oishi, Yoshie; Dohmoto, Nobuhiko

doi:10.3177/jnsv.55.156

Cited by 21 publications

(19 citation statements)

References 15 publications

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“…Multiple studies have assessed the health benefits of seafood EPA and DHA. Moreover, dietary proteins and hydrolyzates from fish and shellfish have been reported to improve blood lipid contents (Hosomi and others ; Vikoren and others ), hepatic fat accumulation (Maeda and others ), and body weight gain (Oishi and Dohmoto ) in humans and experimental animals. However, the potential health functions of fish tissues other than muscle have attracted little attention.…”

Section: Introductionmentioning

confidence: 99%

Salmon Protamine Decreases Serum and Liver Lipid Contents by Inhibiting Lipid Absorption in an In Vitro Gastrointestinal Digestion Model and in Rats

Hosomi

Miyauchi

Yamamoto

et al. 2015

Journal of Food Science

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Protamine has been used as an antiheparin drug and a natural preservative in various food products. However, limited studies have evaluated the physicochemical and functional properties of protamine. Hence, we assessed the mechanisms underlying the inhibition of lipid absorption following salmon protamine intake in in vitro and in vivo studies. In initial experiments, a salmon protamine hydrolyzate (PH) was prepared using in vitro simulated gastrointestinal digestion suppressed pancreatic lipase activity and micellar cholesterol solubility. This PH had higher bile acid-binding capacity and emulsion breakdown activity than casein hydrolyzate and l-arginine. However, the hypolipidemic functions of protamine were dramatically reduced by pancreatin digestion. In further experiments, groups of male Wistar rats were fed an AIN-93G diet containing 5% (wt/wt) salmon protamine or a protamine amino acid mixture. After 4 wk of feeding with experimental diets, reductions in serum and liver triacylglycerol (TAG) and cholesterol contents were observed in the presence of protamine, reflecting inhibition of TAG, cholesterol, and bile acid absorption. These data suggest that the formation of insoluble PH-bile acid complexes is critical before the bile acid-binding capacity is reduced. Therefore, dietary salmon protamine may ameliorate lifestyle-related diseases such as hyperlipidemia and obesity.

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Section: Introductionmentioning

confidence: 99%

Salmon Protamine Decreases Serum and Liver Lipid Contents by Inhibiting Lipid Absorption in an In Vitro Gastrointestinal Digestion Model and in Rats

Hosomi

Miyauchi

Yamamoto

et al. 2015

Journal of Food Science

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“…Previous studies have shown that, in contrast to casein, dietary fish protein decreases blood cholesterol in laboratory animals (Zhang and Beynen 1993; Wergedahl and others 2004; Shukla and others 2006; Hosomi and others 2009; Kato and others 2009). In addition, dietary fish protein has other beneficial effects such as anti‐hypertensive and anti‐obesity properties (Boukortt and others 2004; Oishi and Dohmoto 2009). …”

Section: Introductionmentioning

confidence: 99%

Fish Protein Decreases Serum Cholesterol in Rats by Inhibition of Cholesterol and Bile Acid Absorption

Hosomi¹,

Fukunaga²,

Arai³

et al. 2011

Journal of Food Science

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Fish protein has been shown to decrease serum cholesterol content by inhibiting absorption of cholesterol and bile acid in laboratory animals, though the mechanism underlying this effect is not yet fully understood. The purpose of this study was to elucidate the mechanism underlying the inhibition of cholesterol and bile acid absorption following fish protein intake. Male Wistar rats were divided into 2 dietary groups of 7 rats each, 1 group receiving a diet consisting of 20% casein and the other receiving a diet consisting of 10% casein and 10% fish protein. Both experimental diets also contained 0.5% cholesterol and 0.1% sodium cholate. After the rats had been on their respective diets for 4 wk, their serum and liver cholesterol contents and fecal cholesterol, bile acid, and nitrogen excretion contents were measured. Fish protein consumption decreased serum and liver cholesterol content and increased fecal cholesterol and bile acid excretion and simultaneously increased fecal nitrogen excretion. In addition, fish protein hydrolyzate prepared by in vitro digestion had lower micellar solubility of cholesterol and higher binding capacity for bile acids compared with casein hydrolyzate. These results suggest that the hypocholesterolemic effect of fish protein is mediated by increased fecal cholesterol and bile acid excretion, which is due to the digestion products of fish protein having reduced micellar solubility of cholesterol and increased bile acid binding capacity.

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“…Compared to casein, APP reportedly inhibits visceral fat accumulation, hypocholesterolaemic effects, and hypotriglyceridemic effects (7,13,15). Although it has been reported that APP's hypocholesterolaemic effect may be induced by increasing the excretion of bile acids (7,8), and/or by increasing cholesterol synthesis in the liver (15), the mecha-analyses of casein and APP sources were performed by Japan Food Research Laboratories (Tokyo), as in a previous report (13). Non-digestive protein and ash amount were determined by using commercial diagnostic kits (Dietary Fiber Assay Kit; Wako Pure Chemical Industries, Osaka, Japan) at Ehime University.…”

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confidence: 99%

Fast-twitch muscle hypertrophy partly induces lipid accumulation inhibition with Alaska pollack protein intake in rats

et al. 2010

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Fish protein is a source of animal protein that is consumed worldwide. Although it has been reported that the intake of Alaska pollack protein (APP) reduces serum triglyceride and body fat accumulation in rats, the mechanisms underlying these effects are poorly understood. In the present study, we fed 5-week-old male Sprague-Dawley rats a high-fat diet with APP or casein for 4 weeks. We reconfirmed that the intake of APP decreases serum triglycerides and inhibits visceral body fat accumulation in rats. We found that APP had a higher non-digestive protein content than casein, and the amount of protein in feces was higher in the APP group than in the casein group. However, the amount of total lipids in feces did not differ significantly between the groups. We also found that the gastrocnemius muscle, a fast-twitch muscle, tended to increase in weight, and that the epididymal fat weight correlated negatively with gastrocnemius muscle weight in the APP group. These results imply that the enhancement of basal energy expenditure by fast-twitch muscle hypertrophy, rather than the enhancement of lipid excretion via feces, partly causes APP-induced inhibition of lipid accumulation in rats.

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Alaska Pollack Protein Prevents the Accumulation of Visceral Fat in Rats Fed a High Fat Diet

Cited by 21 publications

References 15 publications

Salmon Protamine Decreases Serum and Liver Lipid Contents by Inhibiting Lipid Absorption in an In Vitro Gastrointestinal Digestion Model and in Rats

Salmon Protamine Decreases Serum and Liver Lipid Contents by Inhibiting Lipid Absorption in an In Vitro Gastrointestinal Digestion Model and in Rats

Fish Protein Decreases Serum Cholesterol in Rats by Inhibition of Cholesterol and Bile Acid Absorption

Fast-twitch muscle hypertrophy partly induces lipid accumulation inhibition with Alaska pollack protein intake in rats

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