The present study covers the myxomycetes obtained from the natural environment with the Humidity Chamber Technique at Eşmişek Plateau in 2018 and 2019. From the field and laboratory studies, 35 species were identified belonging to16 genera within the 9 families. Only two species were collected in natural environment, while 33 species were obtained with the Humidity Chamber Technique. In the present study, it was determined that Didymium difforme (Pers.) Gray, Arcyria cinerea (Bull.) Pers, Comatricha nigra (Pers.) J. Schröt, C. ellae Härk. were the most prevalent species.