Aktivitas Superoksida Dismutase Tikus Diabetes Yang Diberi Ekstrak Batang Kapulaga Dan Glibenklamid

Rahmawati, Gisti; Rachmawati, Farida Nur; Winarsi, Hery

doi:10.20884/1.sb.2014.1.3.42

Cited by 8 publications

(8 citation statements)

References 14 publications

(15 reference statements)

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“…Cu 2+ and Zn 2+ are required as enzyme stabilizers. SOD function to accelerate O2 dismutation and maintain balance between the amount of O2 and H2O2 formation (Rahmawati et al 2014). The result of SOD activity measurement showed that SNEDDS ginger extract with eel fish oil at the same dose with pure ginger extract has higher SOD activity.…”

Section: Discussionmentioning

confidence: 99%

Total phenolic content and antioxidant activity of ginger extract and SNEDDS with eel fish bone oil (Anguilla spp.)

et al. 2018

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Wijayanti II, Budiharjo A, Pangastuti A, Prihapsara F, Artanti AN. 2018. Total phenolic content and antioxidant activity of ginger extract and SNEDDS with eel fish bone oil (Anguilla spp.). Nusantara Bioscience 10: 164-169. Aims of this study were to measure phenolic content of ginger (Zingiber officinale) and to observe the comparison of antioxidant activity between pure ginger extract and ginger extract in SNEDDS (Self Nanoemulsifying Drug Delivery System) with bone of eel fish oil (Anguilla spp.) as a conductor. Analysis was done based on IC50 value using DPPH (2,2 diphenyl-1-picrylhydrazyl) method and activity of Superoxide Dismutase (SOD) in vivo using Adrenochrome Assay. Determination of total phenolic content of ginger extract was measured by UV/Vis Spectrophotometric using Folin-Ciocalteau reagents then the absorbances were measured at wavelength 756 nm. Calculation of IC50 DPPH of the ginger extract and SNEDDS were measured using spectrophotometer UV/Vis at 517 nm wavelength. Measurements of SOD was based on the ability of SOD inhibit spontaneous auto reduction of epinephrine using Adrenochrome Assay at 12 male Wistar rats that divided into 4 treatment groups with 3 replicates for each group. Total phenolic content of ginger extract from 3 replications were 4,8519 ± 0,037 µg/mL equivalent with gallic acid standard solutions (GAE) or 485,1847 ± 3,7045 mgGAE/100 g dry weight. Antioxidant scavenging activity by DPPH assay of ginger was higher than SNEDDS. IC50 of ginger was 385.4 mg/mL, and IC50 of SNEDDS was 428.4 mg/mL. The result of descriptive analysis of Kruskal Wallis of SOD activity assay indicated significant differences between treatment groups (P = 0.001 (P <0.05)). SOD activity test performed in vivo showed that ginger extract made into SNEDDS system with eel fish oil used as the conductor has a significant influence compared with ginger extract group.

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Section: Discussionmentioning

confidence: 99%

Total phenolic content and antioxidant activity of ginger extract and SNEDDS with eel fish bone oil (Anguilla spp.)

et al. 2018

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“…A preliminary phytochemical analysis of the EESL was shown flavonoids, steroids/triterpenoids, tannins, glycosides and saponins. Flavonoids, their glycosides and saponins are responsible for blood glucose decreasing activity through increased insulin secretion, as shown in an experiment by NA-STZ induced diabetic rats, which is capable of stimulating pancreatic secretion [10], [11].…”

Section: Discussionmentioning

confidence: 99%

Effect of Saurauia vulcani Korth. Leaves on Superoxide Dismutase, HbA1c Levels and Insulin Expression in Hyperglycemic Rats

Surbakti¹,

Sitorus²,

Rosidah³

et al. 2019

Open Access Maced J Med Sci

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AIM: This study aimed to investigate Saurauia vulcani Korth. leaves. the activity of ethanol extract in hypoglycemic, superoxide dismutase (SOD), glycosylated haemoglobin (HbA1c) and detection of insulin expression by immunochemistry METHODS: Saurauia vulcani Korth. Leaves powder was extracted by maceration method with ethanol 96%. The extract was administrated orally in doses of 100 mg/kg BW for 27 days. Diabetes was induced in rats by administered of Nicotinamide (NA) 230 mg/kg BW and streptozotocin (STZ) 65 mg/kg BW. Level of blood glucose, SOD, HbA1c were measured, and histopathology pancreas was observed to determine insulin expression with immunochemistry RESULTS: Ethanol extract of Saurauia vulcani Korth. Leaves (EESL) shown a significantly (*p < 0.05) reduced in blood glucose levels at 104.25 ± 2.562 mg/dL and HbA1c level at 32.53 ± 0.188 ng/mL, but increased SOD level at 60.64 ± 0.740 pg/mL and histopathology study shown secretion insulin as seen number of expressions of insulin / slice 31.00 ± 0.315. CONCLUSION: The result of this study showed EESL possess the hypoglycemic activity and increase the level of SOD but decrease the level of HbA1c in diabetic rat condition. The mechanism of the activity is suggested by stimulating the insulin secretion of pancreas β-cells which were damaged.

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“…According to Rahmawati et al (2014), vitamin C helps in reducing the amount of superoxide anion radicals and increasing SOD activity by inhibiting the protein glycation process. In addition, vitamin C can act as an exogenous antioxidant which can substitute the role of endogenous antioxidants if they are low in the body.…”

Section: Resultsmentioning

confidence: 99%

Antidiabetic Potentials of Button Mushroom (Agaricus bisporus) on Alloxan-Induced Diabetic Rats

et al. 2018

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Button mushrooms (Agaricus bisporus) is an edible mushroom that is most widely cultivated in the world. It contains bioactive compounds that might provide beneficial effects on diabetes mellitus patient. The study aimed to determine the effects of A. bisporus administration on the blood glucose, and malondyaldehyd (MDA) levels as well as superoxide dismutase (SOD) activity of alloxan-induced diabetic rats. This study was also conducted to determine the secondary metabolites produced by A. bisporus. The method used was experimental methods with Completely Randomized Design. A. bisporus extract at the doses of 250, 500 and 750 mg/kg body weight (BW) per day were orally applied to alloxan-induced diabetic rats for a period of 14 days after the rats became diabetes. The results showed that the extract of A. bisporus could decrease blood glucose, and MDA levels as well as increase SOD activity (p < 0.05). A. bisporus extract 500 mg/kg BW is the most effective dose to be used. Based on Thin Layer Chromatography (TLC) test, it was known that secondary metabolites produced by A. bisporus are flavonoids, alkaloids, terpenoids and saponins. A. bisporus has potential as an antidiabetic through the ability to decrease blood glucose, and MDA levels, as well as increase SOD activity in diabetic rats. This research is able to provide information about the antidiabetic potential of A. bisporus extract so that it can be used as an alternative natural antidiabetic agent and can be applied in the community with ease and in a more controlled industrial scale.

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Aktivitas Superoksida Dismutase Tikus Diabetes Yang Diberi Ekstrak Batang Kapulaga Dan Glibenklamid

Cited by 8 publications

References 14 publications

Total phenolic content and antioxidant activity of ginger extract and SNEDDS with eel fish bone oil (Anguilla spp.)

Total phenolic content and antioxidant activity of ginger extract and SNEDDS with eel fish bone oil (Anguilla spp.)

Effect of Saurauia vulcani Korth. Leaves on Superoxide Dismutase, HbA1c Levels and Insulin Expression in Hyperglycemic Rats

Antidiabetic Potentials of Button Mushroom (Agaricus bisporus) on Alloxan-Induced Diabetic Rats

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