Aktivitas Proporsi Berbagai Cairan Rumen dalam Mengatasi Tannin  dengan Tehnik In Vitro

Muslim, Gatot; Sihombing, Jobel; Fauziah, Syifa; Abrar, Arfan; Fariani, Armina

doi:10.33230/jps.3.1.2014.1727

Cited by 8 publications

(7 citation statements)

References 8 publications

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“…Rumen microbes reuse the N-NH 3 formed to build body cells (Wahyuni et al 2014). The greater the bacterial population is, the greater the digestibility of feed (Muslim et al 2014). a,b,c Means in the same column with different letters show significant differences (P<0.05).…”

Section: In Vitro Rumen Fermentation and Digestibility Assaymentioning

confidence: 99%

Ruminal fermentation characteristics and methane emissions with the addition of Cassia spp. extract to a total mixed ration based on corn stover

Wahyuni,

Sunarso,

Prasetiyono

et al. 2024

J. Anim. Behav. Biometeorol.

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This study evaluated the effects of the addition of four different Cassia species extracts to a total mixed ration (TMR) composed of corn stover on the characteristics and methane emissions in vitro. The design used was a randomized complete block design (RCBD), with the source of inoculum derived from rumen fluid taken from three sheep serving as groups in the treatment. The concentrations of Cassia siamea, C. fistula, C. surattensis, and C. spectabilis extracts added to the TMR based on corn stover were 2 mg/mL. Every treatment was repeated three times. The variables observed were in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD); microbial protein synthesis (MPS); volatile fatty acid (VFA) content; acetate, propionate, and butyrate levels; gas production; methane; and populations of methanogen bacteria and protozoa. The study showed that the addition of Cassia spp. extract to TMR at a dose of 2 mg/mL increased (P <0.05) the IVDMD, IVOMD, N-NH3, and total bacterial population but decreased the total and partial VFA, methanogenic bacteria, and the number of protozoa. The addition of C. surattensis extract reduced methane emissions by 13.11% compared with those of the controls based on the percentage of total gas or 14.9% based on mL/g DOM (P <0.05). In conclusion, Cassia spp. extract is a potential agent for reducing methanogenesis in the rumen.

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Section: In Vitro Rumen Fermentation and Digestibility Assaymentioning

confidence: 99%

Ruminal fermentation characteristics and methane emissions with the addition of Cassia spp. extract to a total mixed ration based on corn stover

Wahyuni,

Sunarso,

Prasetiyono

et al. 2024

J. Anim. Behav. Biometeorol.

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“…Kajian in vitro ransum percobaan pada penelitian ini menghasilkan gambaran umum, yaitu populasi bakteri, protozoa, asam lemak terbang dan ammonia dalam cairan rumen sapi perah, yaitu berturut-turut pada kisaran 17,30-25,20 10 10 sel/mL; 5,00-12,40 10 6 sel/mL; 130,00-148,80 mM, dan 5,35-7,54 mM. Populasi bakteri dan protozoa masih dalam kisaran normal karena menurut Muslim et al, (2014) populasi bakteri dan protozoa berkisar 10 10 sampai 10 11 sel/mL dan 10 5 -10 6 sel/mL. Kondisi ini juga sama dengan produk fermentasi, yaitu asam lemak terbang (130,00-148,80 mM) dan ammonia (5,35-7,54 mM) yang menunjukkan jumlah yang normal pada kisaran 70-150 mM (McDonald et al, 2011) dan 4,45-7,33 mM (Dhia et al, 2019.…”

Section: Hasil Dan Pembahasanunclassified

Fermentabilitas Ransum Sapi Perah Berbasis Jerami Padi dan Daun Kaliandra yang Disuplementasi Konsentrat Terfermentasi

Hidayat

Sudana

Tanuwiria

et al. 2021

jupet

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ABSTRAK. Penelitian bertujuan untuk mengetahui fermentabilitas ransum berbasis jerami padi dan kaliandra yang mengandung konsentrat terfermentasi (in vitro). Penelitian dilakukan secara eksperimental dengan rancangan acak lengkap yang terdiri dari 4 perlakuan dan 5 ulangan. Data dilakukan sidik ragam dan uji Duncan. Ransum perlakuan adalah P0 = 39% jerami padi + 21% daun Kaliandra + 40% konsentrat, P1 = 39% jerami padi + 21% daun Kaliandra + 40% konsentrat fermentasi, P2 = 44% jerami padi + 21% daun Kaliandra + 35% konsentrat fermentasi, P3 = 49% jerami padi + 21% daun kaliandra + 30% konsentrat fermentasi. Hasil menunjukan bahwa bakteri dan protozoa rumen menghasilkan populasi yang tidak berbeda nyata, sedangkan konsentrasi asam lemak terbang dan amonia berbeda nyata (P<0,05). Ransum P1 menghasilkan konsentransi asam lemak terbang yang nyata (P<0,05) paling tinggi (148,80 mM) dengan amonia yang paling rendah (5,53 mM). Sementara itu, konsentrasi amonia tertinggi (P<0,05) diperoleh pada perlakuan P0 sebesar 7,54 mM, sebaliknya menghasilkan konsnetrasi asam lemak terbang paling rendah. yaitu 5,35 mM. Kesimpulan ransum dengan komposisi 39% jerami padi, 21% kaliandra, 40% menghasilkan fermentabilitas yang terbaik. Kata kunci : Fermentabilitas, jerami padi, Kaliandra, konsentrat terfermentasi, rumen ABSTRACT. This study aims to evaluate the fermentability of rice straw and Calliandra leaves-based rations supplemented fermented concentrates (in vitro). The research was conducted experimentally with a completely randomized design consisting of 4 treatments and 5 replications. The data were analyzed by variance and Duncan's test. The treatment rations were P0 = 39% rice straw + 21% Calliandra leaves + 40% concentrate, P1 = 39% rice straw + 21% Calliandra leaves + 40% fermented concentrate, P2 = 44% rice straw + 21% Calliandra leaves + 35% fermented concentrate, P3 = 49% rice straw + 21% Calliandra leaves + 30% fermented concentrate. The results showed that the treatment had no significant effect to rumen bacteria and rumen protozoa populations, while the concentrations of volatile fatty acids and ammonia were significantly different (P<0.05). Ration P1 produced the highest (P <0.05) concentration of volatile fatty acids (148.80 mM) with the lowest (P<0.05) ammonia (5.53 mM). Meanwhile, the highest ammonia concentration (P <0.05) was obtained in the P0 treatment of 7.54 mM, on the other hand it resulted in the lowest (P <0.05) concentration of volatile fatty acids, namely 5.35 mM. Conclusion, that rations with a composition of 39% rice straw, 21% Calliandra, 40% fermented concentrate yield the best fermentability.

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“…N-NH 3 mempengaruhi laju pertumbuhan mikroba yang ada didalam cairan rumen, karena N-NH 3 akan digunakan sebagai sumber N untuk memenuhi kebutuhan nutrisinya [6]. N-NH 3 merupakan hasil dari aktivitas fermentasi dalam rumen, yaitu dari degradasi protein yang berasal dari pakan dan sumber nitrogen yang cukup penting untuk sintesis mikroba rumen [13].…”

Section: Konsentrasi Amonia (N-nh 3 )unclassified

pH Inkubasi, Konsentrasi Amonia dan Kecernaan Bahan Organik (KcBO) Uji In Vitro Rumput Odot (Pennisetum purpureum cv. Mott) yang Ditanam pada Tanah Lokasi Bekas Tambang Nikel dengan Aplikasi Berbagai Level Biochar.

Ramadani¹,

Malesi²,

Kurniawan³

2021

JIPHO

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Penelitian ini bertujuan untuk mengevaluasi nilai kecernaan rumput odot(Pennisetum purpureum cv. Mott) yang ditanam di lokasi tanah bekas tambang nikeldengan aplikasi berbagai level biochar. Rancangan yang digunakan dalam penelitian iniadalah rancangan acak lengkap yang terdiri dari 4 perlakuan dan 4 kali ulangan untukmasing-masing perlakuan. Perlakuan yang digunakan dalam penelitian ini yaitu A0(kontrol, tanpa biochar), A1 (5-ton ha-1), A2 (10-ton ha-1) dan A3 (15-ton ha-1) biochar.Parameter yang diamati dalam penelitian ini terdiri atas pH inkubasi, konsentrasi amonia(N-NH3) dan kecernaan bahan organik. Data yang diperoleh dianalisis dengan analisisragam sesuai dengan rancangan acak lengkap (RAL) dan apabila berpengaruh nyata makadilakukan uji beda antar perlakuan dengan menggunakan uji Duncan Multiple Range Test(DMRT) menggunakan SPSS for window 16. Berdasarkan hasil penelitian dapatdisimpulkan bahwa perlakuan tidak berpengaruh terhadap parameter penelitian.

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Aktivitas Proporsi Berbagai Cairan Rumen dalam Mengatasi Tannin dengan Tehnik In Vitro

Cited by 8 publications

References 8 publications

Ruminal fermentation characteristics and methane emissions with the addition of Cassia spp. extract to a total mixed ration based on corn stover

Ruminal fermentation characteristics and methane emissions with the addition of Cassia spp. extract to a total mixed ration based on corn stover

Fermentabilitas Ransum Sapi Perah Berbasis Jerami Padi dan Daun Kaliandra yang Disuplementasi Konsentrat Terfermentasi

pH Inkubasi, Konsentrasi Amonia dan Kecernaan Bahan Organik (KcBO) Uji In Vitro Rumput Odot (Pennisetum purpureum cv. Mott) yang Ditanam pada Tanah Lokasi Bekas Tambang Nikel dengan Aplikasi Berbagai Level Biochar.

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