Aktivitas Promoter â-aktin Ikan Medaka Jepang (Oryzias latipes) pada Ikan Mas (Cyprinus carpio)

Abstract

This study was conducted to examine activity of medaka (Oryzias latipes) â-actin promoter (mBP) in common carp (Cyprinus carpio) as the first step towards development of common carp transgenic in country. Gene construct pmBP-hrGFP that consists of mBA promoter and humanized Renilla reniformis green fluorescent protein gene (hrGFP) was injected into cytoplasm of one cell stage of common carp by using microinjector. PmBP-hrGFP concentration used for microinjection was 50 µg/mL aquabides. Parameters observed were survival rate of embryo (SRe), hatching rate (HR) and expression of hrGFP gene. SRe was calculated before eggs hacthed, while hatching rate (HR) was after all of eggs hatched. The activity of mBA promoter was analyzed by observation of hrGFP gene transient expression using a fluorescence microscope. The results of experiment showed that SRe (87,5%) and HR (79.2%) of control was respectevily higher than that of injected treatment (75.0% & 61.7%). Expression of hrGFP was observed firstly at blastula (12 hours after fertilization) to 1-day-old larval stages (24 hours after hatching) with higher gene expression at blastula to late gastrula stages. Percentage of micronjected larvae expressing hrGFP at 6 hours after hatching reached 71.6 ± 6.7%. Conclusion was that mBA promoter could drove hrGFP expression in common carp, hence it can be used to produce common carp transgenic by changing hrGFP with genes correlated with important traits in aquaculture.