AKTIVITAS INHIBISI α-GLUKOSIDASE DARI EKSTRAK AIR DAN ETANOL DAUN SIMPUR AIR (Dillenia suffruticosa (Griff.) Martelli)

Yuningtyas, Sitaresmi; Roswiem, Anna P.; Erfina, Erfina

doi:10.47219/ath.v3i1.23

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…The sample was identified in the Research Center for Plant Conservation and Botanic Gardens, Indonesian Institute of Sciences (LIPI), Bogor. The fresh leaves of akar kaik-kaik were cleaned with fresh water from the tap and dried for 14 days with airdried method [9][10][11]. The dried leaves then powdered using blender and homogenized by sieving using mesh 60.…”

Section: Preparation and Extraction Of Akar Kaik-kaik Leavesmentioning

confidence: 99%

Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans

Syafriana¹,

Setyaningsih²,

Rachmawani³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Akar kaik-kaik (Uncaria cordata) or known as hook vine is one of the medicinal plants whose leaves are used in traditional medicine as an antidiabetic by the people of Riau. Other than that, this plant also has potential as an antimicrobial agent due to the dominant of alkaloid content possessed by members of the genus Uncaria. This research aims to study the antimicrobial activity from the ethanol extract of akar kaik-kaik leaves against Staphylococcus aureus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria), and Candida albicans (yeast). The extraction was carried out using the maceration method and a solvent of 70% ethanol. The extract then tested for phytochemical screening to find out the secondary metabolite. After that, the extract was tested for antimicrobial activity. The test was done by Kirby-Bauer Method at Nutrient Agar (NA) for bacteria, and Sabouraud Dextrose Agar (SDA) for yeast. The extract contained alkaloid, saponin, and tannin according to the phytochemical analysis. The antimicrobial activity showed that the extract can inhibit the growth of S. aureus at a concentration of 10%, 20%, and 40% respectively about 6.91 ± 0.04 mm, 8.51 ± 0.14 mm, and 10.89 ± 1.09 mm. Meanwhile, the extract cannot inhibit the growth of E. coli and C. albicans.

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Section: Preparation and Extraction Of Akar Kaik-kaik Leavesmentioning

confidence: 99%

Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans

Syafriana¹,

Setyaningsih²,

Rachmawani³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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“…Local societies in Brunei and Malaysia are used D. suffruticosa leaves to promote wound healing, treat fever, and relieve rheumatism 8, 9 . The people in Bangka-Belitung, Sumatra, usually used the boiled water of D. suffruticosa leaves to treat diabetes mellitus 10 . Besides that, the local community also used boiled water of D. suffruticosa leaves as an anti-diarrhea.…”

mentioning

confidence: 99%

Antimicrobial Activity of Ethanolic Extract of Sempur (Dillenia suffruticosa (Griff.) Martelli) Leaves against Pathogenic Microorganisms

et al. 2021

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Sempur (Dillenia suffruticosa) leaves are known as a traditional medicine for the people of Bangka-Belitung Island. The local people empirically utilize the boiled water of D. suffruticosa leaves as anti-diarrhea. However, the antimicrobial activity of the ethanol extract of D. suffruticosa leaves has not been reported. This study aims to determine the antimicrobial activity of the ethanol extract of D. suffruticosa leaves against several microorganisms: Staphylococcus aureus as Gram-positive bacteria, Escherichia coli as Gram-negative bacteria, and Candida albicans as fungi. Extraction was carried out by maceration method with 70% ethanol, then screened for phytochemical constituents. The antimicrobial test was carried out by the disc diffusion method using Nutrient Agar (NA) for bacteria, and Sabouraud Dextrose Agar (SDA) for fungi. The results of phytochemical screening showed that the ethanol extract of D. suffruticosa leaves contained alkaloids, flavonoids, tannins, and saponins. The antimicrobial test showed that the extract of D. suffruticosa leaves could inhibit the growth of S. aureus at concentrations of 10%, 20%, and 40% were 8.35±0.05; 9.34±0.32; and 10.52±0.22, respectively. The ethanol extract of D. suffruticosa leaves could inhibit the growth of S. aureus, whereas E. coli and C. albicans did not show any activity.

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“…Suku Dayak di Kabupaten Bengkayang, Kalimantan Barat biasanya menggunakan rebusan daun muda simpur air untuk mengobati batuk atau berak berdarah (Gunadi et al, 2017). Masyarakat di pulau Bangka Belitung juga sering memanfaatkan air rebusan daun simpur air untuk mengobati diabetes melitus (Yuningtyas et al, 2018) dan sebagai antidiare (Syafriana et al, 2021). Beberapa penelitian terdahulu telah membuktikan bahwa simpur air dapat menjadi bahan antikanker yang potensial.…”

Section: Pendahuluanunclassified

Sitotoksisitas dan Selektivitas Fraksi Kayu Batang Simpur Air (Dillenia suffruticosa (Griff.) Martelli) Terhadap Sel Kanker Payudara

Nurdiani,

Masriani,

Rasmawan

et al. 2023

Al-Kauniyah J. Biol.

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AbstrakKanker payudara didiagnosis menjadi penyebab utama kematian pada wanita di seluruh dunia. Beberapa penelitian terdahulu telah membuktikan bahwa tanaman simpur air (Dillenia suffruticosa (Griff.) Martelli) dapat menjadi agen antikanker yang potensial. Penelitian ini bertujuan untuk menentukan sitotoksisitas dan selektivitas dari fraksi kayu batang simpur air terhadap sel kanker payudara T47D, MCF-7, dan 4T1. Metode penelitian adalah eksperimental laboratorium dan uji aktivitas antikanker terhadap sel kanker payudara dilakukan secara in vitro menggunakan sel kanker payudara T47D, MCF-7, dan 4T1. Proses ekstraksi kayu batang simpur air menggunakan metode maserasi dengan pelarut metanol, selanjutnya difraksinasi dengan pelarut n-heksana dan etil asetat secara berturut-turut dan diuji aktivitas sitotoksiknya dengan metode MTT [3-(4,5-dimetiltiazol-2-il)-2,5 difeniltetrazolium bromida] assay. Hasil yang diperoleh, yaitu fraksi n-heksana menunjukkan sifat sitotoksik sedang pada sel kanker payudara T47D, MCF-7, dan 4T1 dengan nilai IC50 berturut-turut sebesar 65,27 ± 26,17; 66,98 ± 0,97; dan 69,23 ± 6,25 µl/mL dengan IS>2. Dengan demikian, fraksi n-heksana kayu batang simpur berpotensi dikembangkan sebagai antikanker baru.AbstractBreast cancer is diagnosed as the leading cause of death in women worldwide. Several previous studies have proven that the simpur air plant (Dillenia suffruticosa (Griff.) Martelli) can be a potential anticancer agent. This study aims to determine the cytotoxicity and selectivity of the simpur air stem wood fraction against T47D, MCF-7, and 4T1 breast cancer cells. This research is laboratory experimental and the anticancer activity test against breast cancer cells was carried out in vitro. The extraction process for Simpur Air wood stems uses the maceration method with methanol solvent, then fractionated with n-hexane and ethyl acetate solvents successively and tested for cytotoxic activity using the MTT method [3-(4,5-dimethylthiazol-2-yl)-2 .5 diphenyltetrazolium bromide] assay. The results obtained, namely the n-hexane fraction showed moderate cytotoxic properties on T47D, MCF-7, and 4T1 breast cancer cells with IC50 values of 65.27 ± 26.17 respectively; 66.98 ± 0.97; and 69.23 ± 6.25 µl/mL with IS>2. Thus, the n-hexane fraction of simpur stem wood has the potential to be developed as a new anticancer.

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AKTIVITAS INHIBISI α-GLUKOSIDASE DARI EKSTRAK AIR DAN ETANOL DAUN SIMPUR AIR (Dillenia suffruticosa (Griff.) Martelli)

Cited by 3 publications

References 9 publications

Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans

Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans

Antimicrobial Activity of Ethanolic Extract of Sempur (Dillenia suffruticosa (Griff.) Martelli) Leaves against Pathogenic Microorganisms

Sitotoksisitas dan Selektivitas Fraksi Kayu Batang Simpur Air (Dillenia suffruticosa (Griff.) Martelli) Terhadap Sel Kanker Payudara

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