Aktivitas Antioksidan dan Penghambatan Xantin Oksidase Kulit Batang Songi (Dillenia serrata Thunb.)

Abstract

Songi (Dillenia serrata) is a tree endemic to Southeast Sulawesi and its stem bark been used in folk medicine. Nonetheless, only a handful scientific knowledge regarding chemistry and biological activities has been investigated on the plant. The present study aimed to investigate the antioxidant and xanthine oxidase (XO) inhibitory activity of the stem bark of the plant. Methanol extract and organic fractions (petroleum ether, ethyl acetate, and methanol) of the dried powdered stem bark of songi were evaluated for phytochemical screening, total phenolic (TPC), total flavonoid contents (TFC), DPPH, FRAP, and XO in vitro assays. Flavonoids, tannins, terpenoids, steroids, and saponins present in the extract. TPC and TFC in extract were 59.2 mg GAE/g and 23.4 mg QE/g. The contents in organic fractions were in solvent-dependent manner (methanol>ethyl acetate>petroleum ether). Extract and fractions scavenged DPPH radicals (48.2–59.7%) at 100 μg/mL compared to ascorbic acid, trolox, and gallic acid (90.3–93.8%). FRAP values varied from 0.8–3.4 μg/μg equivalent trolox amount (quercetin and gallic acid were 25.7 dan 32.4 μg/μg, respectively). They also inhibited xanthine oxidase (15.3–50.3%) at 100 μg/mL (allopurinol, 98.2%). The study concluded the potential of methanol extract and organic fraction of the stem bark of songi, thus highlighted the prospect of songi to be used in herbal and drugs development from nature.

Extraction of The Chemical Components of Dengen Leaves (Dillenia serrata Thunb) by MAE Method and Activity Test as Antioxidant and Toxicity

“…Table 2 shows that the ethanol extract of Dengen leaves contains secondary metabolites of alkaloids, flavonoids, saponins, polyphenols, terpenoids, and steroids. Secondary metabolites of this group have also been found in Dengen leaves (Purnawati et al, 2020), Dengen fruit (Bandara et al, 2015); (Gandhi & Mehta, 2013);Suaib, 2021;Illing et al, 2017;Illing et al, 2018;Illing et al, 2019;), and Dengen bark (Sabandar et al, 2020).…”

Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits

“…The phytochemicals contained in ethanol extract and organic fractions of M. scabra were screened according to our previous method 5 . The screening was carried out to detect the presence of alkaloids, tannins, flavonoids, terpenoids, steroids, and saponins.…”

“…According to our previous study with modifications 5,7 , total phenolics in the ethanol extract and organic fractions of M. scabra fruits were evaluated spectrophotometrically using the Folin-Ciocalteu (FC) method. A linear gallic acid standard curve was first established within a concentration range of 6.25 to 100 µg/mL versus absorbance of molybdenum-tungsten blue complex at 635 nm.…”

“…Total flavonoids content in the ethanol extract and organic fractions of M. scabra fruits was evaluated spectrophotometrically using the aluminum chloride method as previously reported with modifications 5,7 . A linear quercetin standard curve was first established within a concentration range of 1.56 to 100 µg/mL versus the absorbance of the yellow complex at 425 nm.…”

“…DPPH assay was carried out to determine the radical scavenging activity of ethanol extract and organic fractions of M. scabra fruits towards free radicals 5,7 . The assay was performed qualitatively using dot-blot staining on TLC plate and quantitatively using the spectrophotometric method.…”