Aktivitas antibiofilm ekstrak etanol kulit bawang merah (Allium cepa L.) dalam menghambat pembentukan biofilm Staphylococcus aureus ATCC 25923

Rosyada, Alya Ghina; Prihastuti, Christiana Cahyani; Sari, Dwi Nur Indah; Setiawati, Setiawati; Ichsyani, Meylida; Laksitasari, Anindita; Andini, Restian Febi; Kurniawan, Aris Aji

doi:10.24198/jkg.v35i1.42451

J Ked Gi Unpad

2023

DOI: 10.24198/jkg.v35i1.42451

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Aktivitas antibiofilm ekstrak etanol kulit bawang merah (Allium cepa L.) dalam menghambat pembentukan biofilm Staphylococcus aureus ATCC 25923

Alya Ghina Rosyada

Christiana Cahyani Prihastuti

Dwi Nur Indah Sari

et al.

Abstract: Permasalahan gigi banyak disebabkan oleh biofilm bakteri di dalam rongga mulut. Salah satu bakteri penyusun biofilm yaitu Staphylococcus aureus merupakan jenis bakteri Gram positif yang bersifat patogen oportunistik serta memiliki mekanisme pembentukan biofilm yang kompleks. Kulit bawang merah mengandung senyawa fitokimia seperti flavonoid, saponin, fenol, tanin, alkaloid, dan steroid yang dilaporkan bersifat antibiofilm sehingga berpotensi sebagai alternatif obat kumur. Penelitian ini bertujuan untuk menganal… Show more

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2024

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Cited by 3 publications

References 34 publications

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Assessment of the potential susceptibility of planktonic cells and bacterial biofilms by diosgenin

Costa,

Pereira da Silva,

Santos Araújo

et al. 2024

Food Bioscience

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Assessment of the potential susceptibility of planktonic cells and bacterial biofilms by diosgenin

Costa,

Pereira da Silva,

Santos Araújo

et al. 2024

Food Bioscience

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Antibacterial and Antibiofilm Activity of Ethanol Extract of Batak Onion Bulbs (Allium chinense G.Don.) against Streptococcus mutans and Enterococcus faecalis.

Octiara,

Sipangkar,

Prist

2024

Biomed. Pharmacol. J.

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Background: S.mutans is the main pathogen causing caries, while E.faecalis is the dominant microorganism in dental root canals. Batak onion is one of Indonesia's biological resources frequently used as condiments by Batak tribes and possesses antibacterial compounds such as alkaloids, saponins, and flavonoids. Aims: To analyze antibacterial activity test based on Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and antibiofilm from 70% ethanol extract of Batak onion bulbs against S.mutans and E.faecalis. Material and Methods: This type of research is a laboratory experiment with a post-test only control group design. Determination of MIC by Kirby Baurer disc diffusion method, determination of MBC by streaking method, and colony tests are calculated using colony counters, determination of antibiofilm by Static Microtiter Plate Assays method, and checking the optical density at around 600nm. Data analysis of MIC, MBC, and antibiofilm using Oneway Anova and Post Hoc LSD tests. Results: MIC on S.mutans and E.faecalis was at a concentration of 0.78% with an average of one of inhibition of 9.00 ± 0.43 mm and 8.06 ± 0.20 mm; no MBC was found because no group was able to reduce bacteria 98-98%, Batak onion bulb extract has antibiofilm ability starting at a concentration of 0.78% with the ability to reduce S.mutans bacteria by 84.55% and 85.73% on E.faecalis. Conclusion: 70% ethanol extract of Batak onion bulbs can inhibit bacterial growth and biofilm of S.mutans and E.faecalis. The recommended dose for antibacterial and antibiofilm is a 6.25-50% concentration.

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Effectiveness of Batak Leeks Extract Against S. Mutans and E. Faecalis as Antibacterial and Antibiofilm Agent

OCTIARA,

ADINDA,

SEMBIRING

2024

Int J App Pharm

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Objective: S. mutans biofilms have become the main cause of dental caries. Meanwhile, E. faecalis biofilms cause endodontic infection. Batak leeks are native North Sumatra herb that contains antibacterial properties. This study aims to determine Batak leeks extract's antibacterial and antibiofilm effectiveness towards S. mutans and E. faecalis. Methods: This study was a laboratory experimental research with a posttest-only control group design. The sample size was 27, consisting of 3 samples each with concentrations of 0.78%; 1.56%; 3.25%; 6.25%; 12.5%; 25% and 50% Batak leek extract and 3 negative control samples and 3 positive. The Kirby-Bauer disc diffusion method was used to determine MIC, the streaking method to determine MBC and the Static Microtiter Plate Assays method for antibiofilm determination. Data analysis used Oneway ANOVA and Kruskall Wallis tests followed by post-hoc LSD and Mann-Whitney tests. Results: The results showed that Batak leeks extract contained MIC values at 0,78% concentration levels with an average inhibitory zone of 6,80±0,10 mm in S. mutans and E. faecalis. MBC values were undetected because there were no group capable of reducing bacteria at 98-98% and the highest antibiofilm reduction was found in 50% concentration extract with values of 85.58% for S. mutans and 90.29% for E. faecalis. Conclusion: It concluded that 0.78% concentration of Batak leeks extract has an antibacterial effect, but at 50% concentration was the most influential in inhibiting S. mutans and E. faecalis, and antibiofilm ability starts at the concentration level of 1.56%.

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Aktivitas antibiofilm ekstrak etanol kulit bawang merah (Allium cepa L.) dalam menghambat pembentukan biofilm Staphylococcus aureus ATCC 25923

Cited by 3 publications

References 34 publications

Assessment of the potential susceptibility of planktonic cells and bacterial biofilms by diosgenin

Assessment of the potential susceptibility of planktonic cells and bacterial biofilms by diosgenin

Antibacterial and Antibiofilm Activity of Ethanol Extract of Batak Onion Bulbs (Allium chinense G.Don.) against Streptococcus mutans and Enterococcus faecalis.

Effectiveness of Batak Leeks Extract Against S. Mutans and E. Faecalis as Antibacterial and Antibiofilm Agent

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